Protective role of biogenic selenium nanoparticles in immunological and oxidative stress generated by enrofloxacin in broiler chicken

Abstract

Presently most bacteria are becoming antibiotic resistant. Due to this there is a deficiency of potent antibiotics, therefore we have to preserve and improve the efficiency of existing antibiotics by mitigating the side effects. Enrofloxacin (EFX) is an important antimicrobial used in veterinary practice but it is known to exert immune suppression antioxidant stress. In the present study, we report on: (a) the biosynthesis of selenium nanoparticles (Se NPs), and (b) their protective effect in reducing adverse effects of EFX on broiler chicken. A potent bacterial strain, isolated from farm soil, has been identified as Pantoea agglomerans (GenBank: KU500622). It tolerates a high concentration of selenium dioxide (9 mM) and produces Se NPs under aerobic conditions. The obtained Se NPs are amorphous in structure and spherical in shape with sizes of less than 100 nm. The activity of cellular, humoral immune response and enzymatic and non-enzymatic antioxidants, has significantly been decreased as a result of EFX treatment. We investigated that Se NP supplementation greatly restores these values towards the control, and to even higher than those of the control. Adverse effects of EFX are prevented by simultaneous exposure to Se NPs (0.6 mg per kg of feed) in the diet of poultry chicken.

<p>Green Synthesized BSA-Coated Selenium Nanoparticles Inhibit Bacterial Growth While Promoting Mammalian Cell Growth</p>

“…3 One promising area of research is the application of nanotechnology towards selenium fabrication and use. [6][7][8][9][10] Nanoparticles (NPs) are particularly suitable as they possess high surface area to volume ratios, increased surface area, and increased interactions with biological targets, leading to higher availability and absorption by the host. [11][12][13] In addition, selenium NPs (SeNPs) possess lower toxicity than their elemental form; in mice, the LD 50 of selenomethionine was 25.6 mg Se/kg compared with 92.1 mg Se/kg for SeNPs.…”

Effect of different sources of selenium on performance and characteristics of immune system of broiler chickens

“…Although organic selenium has a higher potential for uptake, there are some reports on more favorable performance of mineral selenium with vitamin E on the performance of poultry (Perez et al, 2010). While some researchers believe that applying mineral or organic selenium has no effect on the immune system of chickens (Biswas et al, 2006), numerous studies have shown that a new source called nano-selenium has a more favorable performance in poultry diets (Shirsat et al, 2016).…”

Biogenic selenium and its hepatoprotective activity

“…However, elemental Se particles produced by some bacterial strains have a very low bioavailability over extended periods of time 24 , 25 . BioSeNPs demonstrated antioxidant and anticancer activities in recent studies without mentioning its bioavailability in mice 5 , 26 , 27 .…”