Protective role of antioxidants capacity of Hyrtios aff. Erectus sponge extract against mixture of persistent organic pollutants (POPs)-induced hepatic toxicity in mice liver: biomarkers and ultrastructural study

Abstract

The current study investigates the hepatoprotective effect of Hyrtios aff. Erectus sponge extract against POPs intoxication on endogenous antioxidant enzymes and lipid peroxidation in mice liver tissue. In the present study, the mice BALB/C were assigned into four groups: group I: received saline subcutaneously for 7 days and served as negative control; group II: received subcutaneously for 7 days, 130.6 mg/100 g/b. w/day POPs mixture(mixture of PCB 28, PCB 52,, PCB 101, PCB 118, PCB 153, PCB 138 and PCB 180, alpha-Hexachlorocyclohexane, beta-Hexachloro-cyclohexane, gamma-hexachlorocyclohexane, Aldrin, O,P'-DDE, Dieldrin, P,p DDE, O,P DDD, Endrin, P,p DDD and P,pDDT were extracted from sediments collected from Lake Mariout), and served as induced group; group III: pretreated with Hyrtios aff. Erectus sponge extract for 7 days, as a protection dose and then treated with POPs as group II and served as protective group; and group IV: received i.p Hyrtios aff. Erectus sponge extract of dose 0.7 mg/100 g b.wt/day for 7 days and served as positive control. After 7 days (experimental period), mice were scarified and the liver was harvested for biochemical estimation. Significant reduction in lipid peroxidation (p < 0.002) was noticed compared to POPs-protected group. The antioxidant biomarkers levels were significantly increase as the hepatic GSH and GST increased by 69.9 and 89.9%, respectively. Such increase was accompanied by a decrease in tyrosine kinase activity by 59.82%, additionally remarkable histopathological changes in liver tissue indicate the protective effect of Hyrtios aff. Erectus sponge extract. The results of this study revealed that the Hyrtios aff. Erectus sponge extract has the potential to diminish the destructive effect of POPs intoxication through enhancement of the endogenous antioxidant status. The hepatoprotective activity of Hyrtios aff. Erectus sponge extract is mediated, by the antioxidant effect of its active constituents. The active constituents of Hyrtios aff. Erectus sponge extract were identified by LC-MS/MS.

Phytochemical and pharmacological screening of Sargassium vulgare from Suez Canal, Egypt

“…5(A). The use of marine natural products as therapeutic and cure tools has been started since 1997 with many discoveries [1][2][3][4][5][6][7].The results of the present study revealed that marine derived sargassium from the Suez Canal, especially those belong to genus vulgare, are rich source of structurally novel and biologically potent natural products. This is in agreement with recent studies which have shown that many seaweeds extracts from the Egyptian marine environment along the Mediterranean Sea displayed significant cytotoxic and apoptotic activity [5,6].…”

“…The marine environments are rich in pharmaceutically potent chemicals basically related to polypshenols and sulfated polysaccharides. Recently, significant increase in the number of marine natural products studies have been carried out to extract, isolate and identify new marine and marine-derived natural products [1][2][3][4]. Seaweeds are rich sources of protein, iodine, vitamins, and minerals so; their metabolites have shown promising activities as anti-cancer.…”

Mammalian CST averts replication failure by preventing G-quadruplex accumulation

“…While the lagging strand template is a prime site for G4 accumulation at telomeres and other G-rich sequences, G4 structures also form during transcription [28,57]. They tend to occur on the displaced G-rich strands of R-loops and once formed they stabilize the R-loop [58]. The resulting G4/R-loop combination can then block passage of the replication fork.…”

Chemical and Biochemical Evaluation of Marine Pollution by Polychlorinated Biphenyls and Pesticides in Two Regions Along the Egyptian Mediterranean Sea

“…On the same manner, PCBs represent an important group of POPs, which are believed to be possible carcinogens or mutagens as well as endocrine disruptors [47,48]. PCBs are used in many industrial processes, for example, in dielectric fluids for transformers and capacitors.…”

“…River run off is thought to be one of the major sources of PCBs to the marine environment. Due to their hydrophobic nature, PCBs have strong affinity for particulate material in aquatic ecosystem [48]. The present results for the concentration levels of the investigated PCBs congeners in "Siganus rivulatus" samples collected from Matrouh coast and El-Mex Bay revealed that the concentration range of total PCBs in "Siganus rivulatus" muscles collected from Matrouh coast is 2.19-6.43 ng/g, dry weight, while its range in "Siganus rivulatus" muscles collected from El-Mex Bay is 38.33-57.52 ng/g, dry weight, which is 11.5 fold more than those collected from Matrouh coast.…”