Protective proteins are differentially expressed in tomato genotypes differing for their tolerance to low-temperature storage

Abstract

When stored at low temperature, tomato fruits exhibit chilling injury symptoms, such as rubbery texture and irregular ripening. To identify proteins related to chilling tolerance, we compared two tomato near isogenic lines differing for their texture phenotype at harvest in a fruit-storage trial including two temperatures (4 and 20 degrees C) along several days of conservation. Fruit evolution was followed by assessing fruit color, ethylene emission and texture parameters. The most contrasted samples were submitted to proteomic analysis including two-dimensional electrophoresis and mass spectrometry of protein spots to identify the proteins, whose expression varied according to the genotype or the storage conditions. Unexpectedly, the most firm genotype at harvest was the most sensitive to cold storage. The other genotype exhibited a delay in fruit firmness loss leading to the texture differences observed after 20 days of 4 degrees C storage. The proteome analysis of these contrasted fruits identified 85 proteins whose quantities varied with temperature or genotype. As expected, cold storage decreased the expression of proteins related to maturation process, such as acidic invertase, possibly controlled post-translational regulation of polygalacturonase and up-regulated proteins related to freezing tolerance. However, the study point out proteins involved in the differential resistance to chilling conditions of the two lines. This includes specific isoforms among the large family of small heat shocked proteins, and a set of proteins involved in the defense against of the reticulum endoplasmic stress.

The ZmASR1 Protein Influences Branched-Chain Amino Acid Biosynthesis and Maintains Kernel Yield in Maize under Water-Limited Conditions

“…Two-dimensional gel spot digestion and LC-MS/MS analysis were performed as described (Page et al, 2010). A database search was performed with X!Tandem (version 2010.01.01.4; http://www.thegpm.org/tandem/).…”

Morpho-histological analysis of tomato (Solanum lycopersicum L.) plants after treatment with juglone

“…This vegetable species is widely used as an experimental plant in many areas of research and has significantly contributed to our understanding of the mechanisms underlying the influence of the environment on plant growth, development, and response to various external factors such as temperature [9], drought and salinity [10,11], or light conditions [12].…”

Heat stress tolerance responses in developing tomato anthers

“…The results obtained in the present study are similar to those obtained by , where the heat-tolerant genotype is shown to modulate fewer genes in response to heat, but also similar to results on other abiotic stresses (Page et al, 2010;Swindell et al, 2007;Zhou et al, 2007). The most marked differences between the two genotypes can be seen in the heat shock, metabolism and oxido-reduction groups: 32% (HS1) respectively 20% (FR) of the modulated genes belong to the heat shock group, 12% (HS1) respectively 20% (FR) belong to metabolic genes group and 8% (HS1) respectively 10% (FR) belong to the oxido-reduction group.…”