Protective properties of recombinant IgA1 protease from meningococcus

Kotel’nikova, O. V.; Alliluev, A. P.; Drozhzhina, E. Yu.; Королева, И. С.; Ситникова, Е. А.; Zinchenko, Anatoly; Гордеева, Е. А.; Melikhova, T. D.; Nokel, E. A.; Zhigis, L. S.; Zueva, V. S.; Razgulyaeva, O. A.; Serova, O. V.; Yagudaeva, E. Yu.; Румш, Л. Д.

doi:10.1134/s1990750813040033

Cited by 3 publications

(1 citation statement)

References 7 publications

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“…Our team has shown [ 11 , 12 ] that mature IgA1 protease from a live culture of N. meningitidis serogroup A, recombinant variants of the enzyme from meningococcus serogroup B strain H44/76 as well as its mutants of the serine residue of the active center protected mice from infection with virulent culture of meningococci of serogroups A, B and C.…”

Section: Introductionmentioning

confidence: 99%

Highly Similar Sequences of Mature IgA1 Proteases from Neisseria meningitidis, Neisseria gonorrhoeae and Haemophilus influenzae

Karlinsky¹,

Prokopenko²,

Zinchenko³

et al. 2022

Pathogens

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The mature serine-type IgA1 protease from Neisseria meningitidis serogroup B strain H44/76 (IgA1pr1_28–1004) is considered here as the basis for creating a candidate vaccine against meningococcal meningitis. In this work, we examine the primary structure similarity of IgA1 proteases from various strains of a number of Gram-negative bacteria (N. meningitidis, Neisseria gonorrhoeae, Haemophilus influenzae) in order to find a structural groundwork for creating a broad-spectrum vaccine based on fragments of this enzyme. BLAST has shown high similarity between the primary structure of IgA1pr1_28–1004 and hypothetical sequences of mature IgA1 proteases from N. meningitidis (in 1060 out of 1061 examined strains), N. gonorrhoeae (in all 602 examined strains) and H. influenzae (in no less than 137 out of 521 examined strains). For these enzymes, common regions of sequence correspond to IgA1pr1_28–1004 fragments 28–84, 146–193, 253–539, 567–628, 639–795 and 811–1004, with identity of at least 85%. We believe that these fragments can be used in the development of a vaccine to prevent diseases caused by pathogenic strains of N. meningitidis and N. gonorrhoeae as well as a significant number of strains of H. influenzae.

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Section: Introductionmentioning

confidence: 99%

Highly Similar Sequences of Mature IgA1 Proteases from Neisseria meningitidis, Neisseria gonorrhoeae and Haemophilus influenzae

Karlinsky¹,

Prokopenko²,

Zinchenko³

et al. 2022

Pathogens

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Protective potency of recombinant meningococcal IgA1 protease and its structural derivatives upon animal invasion with meningococcal and pneumococcal infections

Kotel’nikova

Alliluev

Zinchenko

et al. 2019

Microbes and Infection

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Potential Polyvaccine Based on Microbial IgA1 Protease for Prophylaxis of Bacterial Meningitis

Alliluev¹,

Kotel’nikova²,

Zinchenko³

et al. 2016

Epidemiology and Vaccine Prevention

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The immunogenic and protective activities of recombinant IgA1 serine protease obtained on the base of the genome DNA of N. meningitidis serogroup B strain H44/76 were studied. A several recombinant proteins of different molecular weights that are based on the full-length primary structure of the enzyme, taking into account the distribution of B- and T-epitopes, also were studied. In experiments on laboratory animals it was shown that a number of tested preparations demonstrate the immunogenic and protective activity to protect mice from lethal challenge with virulent strains of meningococcus serogroups A, B and C, thereby exhibiting polyvaccine properties. The protective role of antibodies against the IgA1 protease was shown when mice were infected by meningococccus serogroup B. The increase in antibodies to the meningococcal IgA1 protease into the blood of rabbits infected with different serotypes of pneumococci has been detected, indicating potential ability of the meningococcal IgA1 protease to generate protection against microbes the virulence of which is caused by IgA1protease.

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Protective properties of recombinant IgA1 protease from meningococcus

Cited by 3 publications

References 7 publications

Highly Similar Sequences of Mature IgA1 Proteases from Neisseria meningitidis, Neisseria gonorrhoeae and Haemophilus influenzae

Highly Similar Sequences of Mature IgA1 Proteases from Neisseria meningitidis, Neisseria gonorrhoeae and Haemophilus influenzae

Protective potency of recombinant meningococcal IgA1 protease and its structural derivatives upon animal invasion with meningococcal and pneumococcal infections

Potential Polyvaccine Based on Microbial IgA1 Protease for Prophylaxis of Bacterial Meningitis

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