Protective oral immunization of chickens against Eimeria tenella with sporozoite surface antigens

Rhalem, Abdelkbir; Sahibi, Hamid; Dakkak, A.; Laurent, Fabrice; Kazanji, Mirdad; Yvore, P.; Pery, P.

doi:10.1016/0165-2427(93)90091-h

Cited by 9 publications

(8 citation statements)

References 27 publications

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“…The considerable protection against infection, evident here in the susceptible strains of orally vaccinated mice, supports earlier work carried out with liposome-entrapped antigens of E. falciformis in mice (10) and of E. tenella in a commercial strain of chickens (11). These results lend credence to the concept of developing an easily mass-administered noninfective vaccine for coccidiosis for domestic animals.…”

Section: Discussionsupporting

confidence: 86%

Oral vaccination against coccidiosis: responses in strains of mice that differ in susceptibility to infection with Eimeria vermiformis

Rose¹,

Hesketh²,

Wakelin³

1997

Infect Immun

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Four strains of mice with different susceptibilities to Eimeria vermiformis were orally dosed with a crude antigen prepared from sporulated oocysts of the parasite, with or without cholera toxin as adjuvant. The effect on subsequent challenge infections depended on the resistance and susceptibility phenotypes of the host: oocyst production was reduced in susceptible C57BL/6 and NIH mice but increased in resistant BALB/c and C3H mice. Despite this contrast, no fundamental differences were detected between the immune responses of BALB/c and C57BL/6 mice, either to vaccination or after superimposed infection, but the suppressing and enhancing effects of vaccination were transmissible to naive recipients via suspensions of mesenteric lymph node cells. The results obtained are compared with those previously reported for parenterally immunized BALB/c and C57BL/6 mice.

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Section: Discussionsupporting

confidence: 86%

Oral vaccination against coccidiosis: responses in strains of mice that differ in susceptibility to infection with Eimeria vermiformis

Rose¹,

Hesketh²,

Wakelin³

1997

Infect Immun

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“…Cell-surface antigens are logical components of vaccines because of their direct role in host-parasite interactions, and promising vaccination results have been observed using both native and recombinant parasite surface antigens. Rhalem et al (1993) obtained 70-88% immune protection against E. tenella challenge by oral vaccination with an extract of the native surface antigen of sporozoites. A cDNA encoding an immunogenic region of a 22 kDa surface protein of E. acervulina sporozoites was cloned and expressed (Jenkins et al, 1989).…”

Section: Surface Antigens As Vaccines Against Eimeriamentioning

confidence: 99%

Vaccines against the avian enteropathogensEimeria,CryptosporidiumandSalmonella

Lillehoj

Yun

Lillehoj

2000

Anim. Health. Res. Rev.

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The worldwide poultry industry provides a substantial proportion of the nutritional requirement of the human population. To keep pace with the increasing demand for the high-quality, low-cost protein source that poultry provides, intensive rearing practices have been developed within the past few decades. For example, chickens are housed routinely in crowded environments under adverse conditions, and genetic strains have been selected for rapid growth, high protein-to-fat content and superior egg-laying characteristics. A major negative consequence of these practices has been an increase in the incidence of diseases. Enteric diseases in particular have emerged as a major problem threatening the future viability of the poultry industry. A variety of methods have been used to combat avian diseases in the commercial setting, including improved farm management practices, the use of antibiotic drugs, the selection of disease-resistant strains of chickens, and the manipulation of the chicken's immune system. In the latter category, the development of vaccines against the major avian diseases has become a priority in the poultry industry. This review will highlight recent progress in vaccine development against three major avian enteric pathogens: Eimeria, Cryptosporidium and Salmonella.

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“…Vaccines with fractioned proteins of the E. tenella sporozoite have been used to test immunoprotection in challenge trials. Almost all of them have been shown to decrease the oocyst shedding of birds significantly [ 6 , 7 , 8 , 9 , 10 , 11 , 12 ]. The sporozoite stage plays a critical role in invasion and likely represents the most amenable target for the host immune response, since in immune birds, they undergo a very restricted development or even fail to penetrate cells in the intestinal tract [ 8 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…Almost all of them have been shown to decrease the oocyst shedding of birds significantly [ 6 , 7 , 8 , 9 , 10 , 11 , 12 ]. The sporozoite stage plays a critical role in invasion and likely represents the most amenable target for the host immune response, since in immune birds, they undergo a very restricted development or even fail to penetrate cells in the intestinal tract [ 8 , 13 , 14 ]. The surface antigens are present on the sporozoite and some of these molecules have roles in host–parasite interactions, probably because these are naturally exposed during parasite recognition and invasion of the intestinal cells [ 8 , 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…The sporozoite stage plays a critical role in invasion and likely represents the most amenable target for the host immune response, since in immune birds, they undergo a very restricted development or even fail to penetrate cells in the intestinal tract [ 8 , 13 , 14 ]. The surface antigens are present on the sporozoite and some of these molecules have roles in host–parasite interactions, probably because these are naturally exposed during parasite recognition and invasion of the intestinal cells [ 8 , 14 , 15 , 16 ]. Until now, it has been difficult to find a single target antigen that can elicit sterilizing immunity against coccidiosis [ 3 , 4 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Protective Immunity Induced by an Eimeria tenella Whole Sporozoite Vaccine Elicits Specific B-Cell Antigens

Juárez-Estrada

Gayosso-Vázquez

Téllez-Isaías

et al. 2021

Animals

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This study investigated protection against Eimeria tenella following the vaccination of chicks with 5.3 × 106 E. tenella whole-sporozoites emulsified in the nanoparticle adjuvant IMS 1313 N VG Montanide™ (EtSz-IMS1313). One-day-old specific pathogen-free (SPF) chicks were subcutaneously injected in the neck with EtSz-IMS1313 on the 1st and 10th days of age. Acquired immunity was assayed through a challenge with 3 × 104 homologous sporulated oocysts at 21 days of age. The anticoccidial index (ACI) calculated for every group showed the effectiveness of EtSz-IMS1313 as a vaccine with an ACI of 186; the mock-injected control showed an ACI of 18 and the unimmunized, challenged control showed an ACI of −28. In a comparison assay, antibodies from rabbits and SPF birds immunized with EtSz-IMS1313 recognized almost the same polypeptides in the blotting of E. tenella sporozoites and merozoites. However, rabbit antisera showed the clearest recognition pattern. Polypeptides of 120, 105, 94, 70, 38, and 19 kDa from both E. tenella life cycle stages were the most strongly recognized by both animal species. The E. tenella zoite-specific IgG antibodies from the rabbits demonstrated the feasibility for successful B cell antigen identification.

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Protective oral immunization of chickens against Eimeria tenella with sporozoite surface antigens

Cited by 9 publications

References 27 publications

Oral vaccination against coccidiosis: responses in strains of mice that differ in susceptibility to infection with Eimeria vermiformis

Oral vaccination against coccidiosis: responses in strains of mice that differ in susceptibility to infection with Eimeria vermiformis

Vaccines against the avian enteropathogensEimeria,CryptosporidiumandSalmonella

Protective Immunity Induced by an Eimeria tenella Whole Sporozoite Vaccine Elicits Specific B-Cell Antigens

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