Protective monoclonal antibodies define maturational and pH-dependent antigenic changes in sindbis virus E1 glycoprotein

Monoclonal Antibodies to the E1 and E2 Glycoproteins of Sindbis Virus: Definition of Epitopes and Efficiency of Protection from Fatal Encephalitis

“…These authors identified three non-overlapping sites (abc, d and e). Based on competitive binding of their MAb 33 with our MAb 106 it can be determined that MAb 106 identifies epitope El-c within a larger complex site encompassing a, b and c (Schmaljohn et al, 1983). Our four MAbs to this site each had distinct patterns of competition, suggesting that the site may be even more complex than previously described.…”

“…Four to eight epitopes have been identified which elicit MAbs with a mixture of neutralizing and haemagglutination-inhibiting functions. The SV E2 MAbs reported originally by Schmaljohn et al (1983) did not identify distinct epitopes, although functional analysis of anti-E2 MAbs by other investigators suggested that distinct epitopes were likely to be present (Roehrig et al, 1980;Stanley et al, 1985Stanley et al, , 1986. Further studies of reactivity of MAbs with different strains of SV (Olmsted et al, 1986;David et al, 1987) and analysis of neutralization escape mutants (Stec et al, 1986) has led to identification of three different regions of antibody reactivity on E2.…”

“…Site El-d was apparently not represented in our panel. MAb K-42 from Schmaljohn et al (1983) defining the El-e epitope competes specifically with MAb 104. El-e elicits antibodies with primarily haemagglutination inhibition activity.…”

“…1) are consistent with this pattern. Selected MAbs were tested for competition using representative MAbs from the anti-E1 MAbs previously described by Schmaljohn et al (1983). These authors identified three non-overlapping sites (abc, d and e).…”

“…A MAb was considered to compete if it consistently inhibited binding of the biotinylated MAb by > 50~. MAbs to SV developed and characterized in the laboratories of Drs Alan Schmaljohn (Schmaljohn et al, 1983) and Robert Johnston (Davis et al, 1987) were kindly provided by these investigators for comparative purposes.…”