Protective mechanism of <i>Astragalus</i> Polysaccharides against Cantharidin‐induced liver injury determined in vivo by liquid chromatography/mass spectrometry metabolomics

Huang, Xiaoduo; Tang, Wenchao; Chang, Lin; Sa, Zongge; Xu, Mengdan; Liu, Jieying; Wang, Lina; Wen, Li; Chen, Yunzhi; Yang, Chunfu

doi:10.1111/bcpt.13585

Cited by 26 publications

(13 citation statements)

References 42 publications

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“…Astragalus is a commonly used traditional Chinese medicine and has been applied clinically ever since a long time ago. With advocating the modernization of traditional Chinese medicine nowadays, as the main active constituent of Astragalus, APS with anti-virus, anti-tumor, anti-aging, anti-radiation, anti-stress and anti-oxidation effects has been paid more attention [10,11] . APS-assisted chemotherapy, as reported, can enhance tumor suppression, reduce toxic and side effects, reinforce the body's cellular immune function and improve the quality of life of patients with tumors [12] .…”

Section: Resultsmentioning

confidence: 99%

Inhibitory Effects of Astragalus Polysaccharide on Proliferation of Human Lung Adenocarcinoma H1299 Cells via the Toll-Like Receptor 4/Myeloid Differentiation Factor 88/Nuclear Factor-Kappa B Signaling Pathway

Chen¹,

Tang²,

Yang³

et al. 2022

IJPS

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To assess the inhibitory effects of Astragalus polysaccharide on the proliferation of human lung adenocarcinoma H1299 cells via the toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-kappa B signaling pathway. H1299 cells were treated with different concentrations of Astragalus polysaccharide. After 24 h and 48 h, cell counting kit-8 assay was employed to measure the cell proliferation. The effects of Astragalus polysaccharide (25 μg/ml) on the invasion ability, migration ability and apoptosis of H1299 cells were determined by transwell assay, wound healing and apoptosis assays, respectively. The changes in expressions of toll-like receptor 4/myeloid differentiation factor 88/nuclear factor-kappa B signaling pathway-related proteins in H1299 cells after 48 h of treatment with 25 μg/ml Astragalus polysaccharide were detected by western blotting. Astragalus polysaccharide inhibited the proliferation of H1299 cells in a dose-dependent manner (p<0.05). The migration rate of H1299 cells treated with 25 μg/ml Astragalus polysaccharide was significantly lower than that of blank control group. After treatment with 25 μg/ml Astragalus polysaccharide, the number of H1299 cells invading through Matrigel into the lower chamber significantly reduced. The apoptosis rate of H1299 cells treated with 25 μg/ml Astragalus polysaccharide was significantly higher than that of blank control group. Toll-like receptor 4, myeloid differentiation factor 88 and nuclear factor-kappa B protein expression levels in H1299 cells treated with Astragalus polysaccharide (25 μg/ml) for 48 h were significantly lower than those of blank control group. Astragalus polysaccharide can inhibit the proliferation, migration and invasion of H1299 cells and induce apoptosis, possibly being related to the reduction of toll-like receptor 4, myeloid differentiation factor 88 and nuclear factor-kappa B protein expressions in cells.

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Section: Resultsmentioning

confidence: 99%

Inhibitory Effects of Astragalus Polysaccharide on Proliferation of Human Lung Adenocarcinoma H1299 Cells via the Toll-Like Receptor 4/Myeloid Differentiation Factor 88/Nuclear Factor-Kappa B Signaling Pathway

Chen¹,

Tang²,

Yang³

et al. 2022

IJPS

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“…They have been implicated in numerous pathophysiological conditions, such as stroke, cancer, and epilepsy. 53 Choline metabolism may occur in the early stages of colon cancer and lead to chronic inflammation, which increases the incidence of colon cancer. The ABC transporter pathway has been reported to play an anti-inflammatory role in mice with colon cancer.…”

Section: Papermentioning

confidence: 99%

Dietary tangeretin improved antibiotic-associated diarrhea in mice by enhancing the intestinal barrier function, regulating the gut microbiota, and metabolic homeostasis

Chen,

Yang,

Zhan

et al. 2023

Food Funct.

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“…The tissues were thawed on ice and grinded with liquid nitrogen. Then the metabolites were extracted (Huang et al, 2021). The repeatability and stability of LC-MS analysis were evaluated using the quality control (QC) sample.…”

Section: Metabolite Extractionmentioning

confidence: 99%

Resveratrol on the Metabolic Reprogramming in Liver: Implications for Advanced Atherosclerosis

Liu

et al. 2021

Front. Pharmacol.

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Background/Aims: Atherosclerosis (AS) is one of the major leading causes of death globally, which is highly correlated with metabolic abnormalities. Resveratrol (REV) exerts beneficial effects on atherosclerosis. Our aim is to clarify the involvement of liver metabolic reprogramming and the atheroprotective effects of REV.Methods: ApoE-deficient mice were administered with normal diet (N), high-fat diet (H), or HFD with REV (HR). Twenty-four weeks after treatment, Oil Red O staining was used to assess the severity of AS. Non-targeted metabolomics was employed to obtain metabolic signatures of the liver from different groups.Results: High-fat diet–induced AS was alleviated by REV, with less lipid accumulation in the lesions. The metabolic profiles of liver tissues from N, H, and HR groups were analyzed. A total of 1,146 and 765 differentially expressed features were identified between N and H groups, and H and HR groups, respectively. KEGG enrichment analysis uncovered several metabolism-related pathways, which are potential pathogenesis mechanisms and therapeutic targets including “primary bile acid biosynthesis,” “phenylalanine metabolism,” and “glycerophospholipid metabolism.” We further conducted trend analysis using 555 metabolites with one-way ANOVA, where p < 0.05 and PLS-DA VIP >1. We found that REV could reverse the detrimental effect of high-fat diet–induced atherosclerosis. These metabolites were enriched in pathways including “biosynthesis of unsaturated fatty acids” and “intestinal immune network for IgA production.” The metabolites involved in these pathways could be the potential biomarkers for AS-related liver metabolic reprogramming and the mechanism of REV treatment.Conclusions: REV exerted atheroprotective effects partially by modulating the liver metabolism.

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Protective mechanism of Astragalus Polysaccharides against Cantharidin‐induced liver injury determined in vivo by liquid chromatography/mass spectrometry metabolomics

Cited by 26 publications

References 42 publications

Inhibitory Effects of Astragalus Polysaccharide on Proliferation of Human Lung Adenocarcinoma H1299 Cells via the Toll-Like Receptor 4/Myeloid Differentiation Factor 88/Nuclear Factor-Kappa B Signaling Pathway

Inhibitory Effects of Astragalus Polysaccharide on Proliferation of Human Lung Adenocarcinoma H1299 Cells via the Toll-Like Receptor 4/Myeloid Differentiation Factor 88/Nuclear Factor-Kappa B Signaling Pathway

Dietary tangeretin improved antibiotic-associated diarrhea in mice by enhancing the intestinal barrier function, regulating the gut microbiota, and metabolic homeostasis

Resveratrol on the Metabolic Reprogramming in Liver: Implications for Advanced Atherosclerosis

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