Protective Immunity to Rotavirus-Induced Diarrhoea Is Passively Transferred to Newborn Mice from Naive Dams Vaccinated with a Single Dose of a Recombinant Adenovirus Expressing Rotavirus VP7sc

Both, Gerald W.; Lockett, Linda J.; Janardhana, Vijayalakshmi; Edwards, Stirling; Bellamy, A R; Graham, Frank L.; Prevec, Ludvik; Andrew, M. E.

doi:10.1006/viro.1993.1203

Cited by 58 publications

(28 citation statements)

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“…We have previously used human Ad5 as a vector to express the rotavirus VP7sc gene (Andrew et al, 1990) inserted into the non-essential E3 region of the Ad genome (Graham & Prevec, 1992). This virus, which is replication-competent in permissive cells, induced a protective immune response in female mice which was passively transferred to the suckling offspring, affording them protection against challenge with the homologous rotavirus (Both et al, 1993). As mice are regarded as a semi-permissive host for human Ad (Mittal et al, 1993) this was an encouraging result.…”

Section: Introductionmentioning

confidence: 97%

Investigation of promoter function in human and animal cells infected with human recombinant adenoviruses expressing rotavirus antigen VP7sc

Krougliak

Prevec

et al. 1995

Journal of General Virology

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) promoter and a modified Ad major late promoter were best, functioning equally well but with different kinetics. In other human cell lines the CMV promoter was more versatile, generally providing sustained expression at a significant level, in one case for at least 6 days. In addition, as mouse, rabbit and pig models of rotavirus infection are under investigation and VP7sc is a vaccine antigen, we also investigated the ability of the recombinant adenoviruses to infect cells from these and other sources. VP7sc expression was detected in several heterologous cell types, illustrating the ubiquity of the human Ad receptor and the versatility of human Ad as vectors when suitable promoters are used.

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Section: Introductionmentioning

confidence: 97%

Investigation of promoter function in human and animal cells infected with human recombinant adenoviruses expressing rotavirus antigen VP7sc

Krougliak

Prevec

et al. 1995

Journal of General Virology

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“…Even though the immunogenicity of baculovirusexpressed VP7 does not seem to be as high as that shown by expressing the protein on the cell surface of adenovirus and vaccinia virus (Andrew et al, 1990(Andrew et al, , 1992Both et al, 1993), the insect system has the advantage of being a particularly safe tool for the preparation of human vaccines. In order to improve protein immunogenicity, use of alternative carriers or adjuvants should be evaluated in further studies.…”

Section: Discussionmentioning

confidence: 99%

Antigenicity, immunogenicity and passive protection induced by immunization of mice with baculovirus-expressed VP7 protein from rhesus rotavirus

Fiore

Dunn²,

Ridolfi³

et al. 1995

Journal of General Virology

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The major neutralization antigen VP7 of rhesus rotavirus (RRV) was expressed in a baculovirus recombinant system. The expressed VP7 showed the same molecular mass as native VP7, and was recognized by hyperimmune sera as well as neutralizing and non-neutralizing monoclonal antibodies (MAbs) raised against RRV. Intraperitoneal administration of the expressed VP7 in mice elicited the production of serum antibodies which were able to immunoprecipitate VP7 from RRV-infected cell lysates and to neutralize the virus in vitro. Sera from immunized mice competed for binding to RRV in an ELISA with both neutralizing and non-neutralizing MAbs specific for VP7. Using a passive protection model of rotavirus disease, vaccination of mice with the recombinant VP7 induced partial protection from infection. These results suggest that the baculovirusexpressed VP7 may be useful in priming a protective immune response to rotavirus infection.

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“…Not only homologous rotaviruses such as EW, ER and YR-1, but also heterologous strains such as SA11 (G3), MO (G3) and a vaccine candidate strain (e.g., RRV, G3) cause gastroenteritis in newborn mice (6)(7)(8)(9). The conventional method of serotyping is the plaque-or focus-reduction neutralization assay.…”

Section: E2 or Bc5 (G3) St-2g7 Or Be18 (G4) Yo-is3 Ku-6b11 Or Yo-mentioning

confidence: 99%

“…Passive immune prophylaxis of rotavirus gastroenteritis was proved to be feasible in suckling mice and infants with lyophilized colostral milk from human rotavirus-immunized cows (6). For experiments with active and passive immunity against rotavirus gastroenteritis, a murine model has been employed by using either homologous or heterologous rotaviruses (6)(7)(8)(9). Epizootic diarrhea of newborn mice, YR-1, was observed in Japan in 1974 (10).…”

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confidence: 99%

Characterization of Vp4 and Vp7 of a Murine Rotavirus (Yr-1) Isolated in Japan

Ushijima

Morikawa

Mukoyama

et al. 1995

JJMSB

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SUMMARY:Nucleotide and deduced amino acid sequences of the outer capsid proteins VP4 and VP7 of a murine rotavirus strain (YR-1) isolated in Japan were determined.Comparisons of the VP7 amino acid sequence of YR-1 with other murine rotavirus strains (EB, EW, EC, EL and EHP) (1) showed that YR-1 was highly homologous to EB, EW, EC, EL and EHP. Moreover, YR-1 was more closely related to strains representing G3 than to any other G type. Analysis of the VP4 amino acid sequence revealed that YR-1 was highly homologous to EB, EW, EC and EL [tentatively P17 (1)], and more closely related to EHP [tentatively P18 (1)] than to any other P type.Enzyme immunoassay with monoclonal antibodies against G types (KU-4 and BH49 for G1, S2-2G10 and BW36 for G2, YO-IE2 and BC5 for G3; and ST-2G7 and BE18 for G4) and against a P type (YO-1S3, KU-12H and YO-2C2 for P8) showed no reactivity.These results indicate that YR-1 is highly homologous to EB, EW, EC and EL.

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Protective Immunity to Rotavirus-Induced Diarrhoea Is Passively Transferred to Newborn Mice from Naive Dams Vaccinated with a Single Dose of a Recombinant Adenovirus Expressing Rotavirus VP7sc

Cited by 58 publications

References 0 publications

Investigation of promoter function in human and animal cells infected with human recombinant adenoviruses expressing rotavirus antigen VP7sc

Investigation of promoter function in human and animal cells infected with human recombinant adenoviruses expressing rotavirus antigen VP7sc

Antigenicity, immunogenicity and passive protection induced by immunization of mice with baculovirus-expressed VP7 protein from rhesus rotavirus

Characterization of Vp4 and Vp7 of a Murine Rotavirus (Yr-1) Isolated in Japan

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