Protective immunity against Trichinella spiralis infection induced by TsNd vaccine in mice

Liu, Pei; Cui, Jingjing; Liu, Ruo Dan; Wang, Min; Jiang, Peng; Liu, Lina; Long, Stuart A.; Li, Ling Ge; Zhang, Shuai Bing; Zhang, Xin Zhuo; Wang, Zhong Quan

doi:10.1186/s13071-015-0791-8

Cited by 47 publications

(43 citation statements)

References 44 publications

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“…Recombinant pcDNA3.1-TsNd vaccine conferred higher levels of protection against T. spiralis infection in comparison to pcDNA3.1-TsE1. Vaccination of mice with pcDNA3.1-TsNd showed 40.44% reduction in worm adults and 53.9% reduction in ML burden with the production of a mixed Th1/Th2 systemic immune response and IgA production at the mucosal level [54]. In this case, the use of pcDNA3.1/TsNd did not increase the protection previously conferred by recombinant TsNd (57.7 and 56.9% reduction in adult and ML burden, respectively) [44].…”

Section: Third-generation Vaccinesmentioning

confidence: 74%

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Vaccination against Trichinella spiralis: Potential, Limitations and Future Directions

Andrade-Becerra¹,

Pompa-Mera²,

MaríaRibas-Aparicio³

et al. 2017

Natural Remedies in the Fight Against Parasites

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Trichinellosis is a food-borne parasitic disease caused by round worms of the genus Trichinella. The majority of human outbreaks are attributed to consumption of raw or undercooked pork meat contaminated with T. spiralis muscle larvae. A blockingtransmission vaccine against trichinellosis will allow preventing swine infection and will contribute to disease control. In this chapter, different vaccine candidates so far developed against T. spiralis, including first-, second-, and third-generation vaccines, are discussed. Most vaccine candidates are based on a unique antigen mainly from the muscle larva stage, inducing with some exceptions, partial protection although a mix Th1/Th2 immune response is elicited. Therefore, the need for identification of new antigens from different parasite stages focusing on infective intestinal larvae, adult, and newborn larvae stages as well as the evaluation of their protective capacity in pigs is presented. The design of multi-epitope vaccines and the use of adjuvants or immunomodulatory molecules capable to polarize the immune response to a Th2-type-protective response are discussed as imperative elements of modern vaccines. Plant-based vaccines and probiotics as excellent tools for vaccine development against T. spiralis are also presented as an attractive platform for veterinary vaccines.

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Section: Third-generation Vaccinesmentioning

confidence: 74%

“…The 31 kDa E/S antigen of ML (TspE1) and TsNd, an up-regulated gene in IIL compared to ML, have been cloned in pcDNA3.1 vector [53,54]. Recombinant pcDNA3.1-TsNd vaccine conferred higher levels of protection against T. spiralis infection in comparison to pcDNA3.1-TsE1.…”

Section: Third-generation Vaccinesmentioning

confidence: 99%

Vaccination against Trichinella spiralis: Potential, Limitations and Future Directions

Andrade-Becerra¹,

Pompa-Mera²,

MaríaRibas-Aparicio³

et al. 2017

Natural Remedies in the Fight Against Parasites

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show abstract

“…8,47,48 It was proved that the protective effect of these genes could be significantly improved by choosing proper methods, such as constituting DNA vaccine or using a prime-boost strategy. [49][50][51] As a recombinant protein candidate antigen for anti-T. spiralis vaccine, rTsserpin showed higher protective effect than many other recombinant protein antigens, such as rNudix, rTsGST and rTspSP-1.3 47,52,53 and should deserve special attention. Ts-serpin is a very promising vaccine candidate for T. spiralis infection, and we intend to try different vaccine type with corresponding delivery route to improve the protective effect of this gene.…”

Section: Discussionmentioning

confidence: 99%

“…Several recombinant protein antigens have been found to show partial protection against T. spiralis infection . It was proved that the protective effect of these genes could be significantly improved by choosing proper methods, such as constituting DNA vaccine or using a prime‐boost strategy . As a recombinant protein candidate antigen for anti‐ T. spiralis vaccine, rTs‐serpin showed higher protective effect than many other recombinant protein antigens, such as rNudix, rTsGST and rTspSP‐1.3 and should deserve special attention.…”

Section: Discussionmentioning

confidence: 99%

Influence of adjuvant formulation on inducing immune response in mice immunized with a recombinant serpin from Trichinella spiralis

Bai

Wang

et al. 2017

Parasite Immunology

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Nematodes of the genus Trichinella are one of the most widespread zoonotic pathogens on the world, and they can still cause major public health problems in many parts of the world. Vaccination against the helminth nematode Trichinella could be a good strategy to reduce the risk of human and animal infection. It was our aim to evaluate three adjuvants, which could be used as an efficient vaccine for animals in combination with rTs-Serpin antigen. In this study, BALB/c mice were vaccinated by an intramuscular route with rTs-Serpin antigen from the parasite Trichinella spiralis in combination with three different adjuvant formulations: Montanide ISA201, Montanide IMS 1313 N PR VG and Freund's complete adjuvant/Freund's incomplete adjuvant (FCA/FIA). The dynamics of IgG, IgM, IgE and cytokine production from spleen cells and worm reduction rate of the vaccinated mice were analysed. The results showed that rTs-serpin can induce partial protection against Trichinella larvae challenge in mice, when compared to the FCA-/FIA-formulated vaccination, the IMS1313 plus rTs-serpin mixture showed higher humoral immunity and similar levels of cellular immunity and worm reduction rate. The study suggested that Montanide IMS nanoparticles 1313 are as effective as FCA but less toxic; thus, Montanide IMS nanoparticles 1313 can be used as a good candidate of adjuvant for developing vaccine against Trichinella spiralis.

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“…The vaccination of mice with the T7 phage-displayed TsNd polypeptides , recombinant TsNd protein (rTsNd) (Long et al 2014) or TsNd DNA (Liu et al 2015a;Liu et al 2015b) produced a partial protection against T. spiralis infection. The results suggested that TsNd might play critical role during the invasion of IECs by T. spiralis.…”

Section: Introductionmentioning

confidence: 99%

The siRNA-mediated silencing of Trichinella spiralis nudix hydrolase results in reduction of larval infectivity

et al. 2015

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Previous studies showed that Trichinella spiralis Nudix hydrolase (TsNd) bound to intestinal epithelial cells (IECs), and vaccination of mice with rTsNd or TsNd DNA produced a partial protective immunity against T. spiralis infection. In this study, three TsNd specific small interfering RNA (siRNA) were designed to silence the expression of TsNd in T. spiralis larvae. SiRNAs were delivered to the larvae by electroporation. Silencing effect of TsNd transcription and expression was determined by real-time PCR and Western blotting, respectively. The infectivity of the larvae treated with siRNA was investigated by the in vitro larval invasion of IECs and experimental infection in mice. The results showed that siRNAs were efficiently delivered into T. spiralis larvae through electroporation. Real-time PCR and Western blotting showed that transcription and expression level of TsNd gene was inhibited 73.3 and 76.7 %, respectively, after being electroporated with 2 μM of siRNA-275 for 1 day. Silencing TsNd expression inhibited significantly the larval invasion of IECs (P < 0.01) and was in a dose-dependent manner (r = -0.97941). The mice with infected larvae treated with TsNd siRNA displayed a 63.6 % reduction in intestinal adult worms and 68.8 % reduction in muscle larval burden compared with mice infected with control siRNA-treated larvae. Our results showed that silencing TsNd expression in T. spiralis significantly reduced the larval infectivity and survival in host.

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Protective immunity against Trichinella spiralis infection induced by TsNd vaccine in mice

Cited by 47 publications

References 44 publications

Vaccination against Trichinella spiralis: Potential, Limitations and Future Directions

Vaccination against Trichinella spiralis: Potential, Limitations and Future Directions

Influence of adjuvant formulation on inducing immune response in mice immunized with a recombinant serpin from Trichinella spiralis

The siRNA-mediated silencing of Trichinella spiralis nudix hydrolase results in reduction of larval infectivity

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