Protective immunity against enteral stages of Trichinella spiralis elicited in mice by live attenuated Salmonella vaccine that secretes a 30-mer parasite epitope fused to the molecular adjuvant C3d-P28

Pompa-Mera, Ericka N.; Arroyo-Matus, Pablo; Ocaña-Mondragón, Alicia; González-Bonilla, César; Yépez‐Mulia, Lilián

doi:10.1016/j.rvsc.2014.09.010

Cited by 37 publications

(49 citation statements)

References 72 publications

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“…In conclusion, performance testing of potential protective antigen is a critical step in vaccine development. As the component of antigen is complex and changes constantly at the three major antigenic stages of T. spiralis , a single molecule was considered not enough to induce effective immunity against T. spiralis . Several recombinant protein antigens have been found to show partial protection against T. spiralis infection .…”

Section: Discussionmentioning

confidence: 99%

“…As the component of antigen is complex and changes constantly at the three major antigenic stages of T. spiralis, a single molecule was considered not enough to induce effective immunity against T. spiralis. 45,46 Several recombinant protein antigens have been found to show partial protection against T. spiralis infection. 8,47,48 It was proved that the protective effect of these genes could be significantly improved by choosing proper methods, such as constituting DNA vaccine or using a prime-boost strategy.…”

Section: Discussionmentioning

confidence: 99%

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Influence of adjuvant formulation on inducing immune response in mice immunized with a recombinant serpin from Trichinella spiralis

Bai

Wang

et al. 2017

Parasite Immunology

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Nematodes of the genus Trichinella are one of the most widespread zoonotic pathogens on the world, and they can still cause major public health problems in many parts of the world. Vaccination against the helminth nematode Trichinella could be a good strategy to reduce the risk of human and animal infection. It was our aim to evaluate three adjuvants, which could be used as an efficient vaccine for animals in combination with rTs-Serpin antigen. In this study, BALB/c mice were vaccinated by an intramuscular route with rTs-Serpin antigen from the parasite Trichinella spiralis in combination with three different adjuvant formulations: Montanide ISA201, Montanide IMS 1313 N PR VG and Freund's complete adjuvant/Freund's incomplete adjuvant (FCA/FIA). The dynamics of IgG, IgM, IgE and cytokine production from spleen cells and worm reduction rate of the vaccinated mice were analysed. The results showed that rTs-serpin can induce partial protection against Trichinella larvae challenge in mice, when compared to the FCA-/FIA-formulated vaccination, the IMS1313 plus rTs-serpin mixture showed higher humoral immunity and similar levels of cellular immunity and worm reduction rate. The study suggested that Montanide IMS nanoparticles 1313 are as effective as FCA but less toxic; thus, Montanide IMS nanoparticles 1313 can be used as a good candidate of adjuvant for developing vaccine against Trichinella spiralis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Influence of adjuvant formulation on inducing immune response in mice immunized with a recombinant serpin from Trichinella spiralis

Bai

Wang

et al. 2017

Parasite Immunology

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“…The identification of native TsE in T. spiralis diverse lifecycle stage worms was performed using an immunofluorescence test (IFT) with worm cross-sections [45,46]. The ML were obtained by artificial digestion of skeletal muscles from experimentally T. spiralis-infected mice at 42 days post-infection (dpi) [47].…”

Section: Recognition Of Native Tse In Worms At Different T Spiralis mentioning

confidence: 99%

Protective immunity in mice vaccinated with a novel elastase-1 significantly decreases Trichinella spiralis fecundity and infection

Zhang

Sun

Bai

et al. 2020

Vet Res

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Trichinella spiralis is an important foodborne parasitic nematode that represents an enormous threat to the food safety of pork meat. The development of a preventive vaccine is valuable for the prevention and control of Trichinella infection in domestic pigs to ensure pork safety. Elastase is a trypsin-like serine protease that hydrolyzes the host's diverse tissue components and participates in parasite penetration, and it might be a novel vaccine target molecule. The aim of this study was to assess the protective immunity produced by vaccination with a novel Trichinella spiralis elastase-1 (TsE) in a mouse model. The results demonstrate that subcutaneous vaccination of mice with rTsE elicited a systemic humoral response (high levels of serum IgG and subclass IgG1/IgG2a and IgA) and significant local enteral mucosal sIgA responses. Anti-rTsE IgG recognized the native TsE at the cuticle, stichosome of intestinal infective larvae and adult worm (AW), and intrauterine embryos of female AW. The rTsE vaccination also produced a systemic and local mixed Th1/Th2 response, as demonstrated by clear elevation levels of Th1 cytokines (IFN-γ, IL-2) and Th2 cytokines (IL-4, IL-10) after spleen, mesenteric lymph node and Peyer's patch cells from immunized mice were stimulated with rTsE. The immunized mice exhibited a 52.19% reduction in enteral AW and a 64.06% reduction in muscle larvae after challenge infection. The immune response triggered by rTsE vaccination protected enteral mucosa from larval intrusion, suppressed larval development and reduced female fecundity. The results indicate that TsE may represent a novel target molecule for anti-T. spiralis vaccines.© The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article' s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article'

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“…The induction of mucosal immunity plays an important aspect to be considered in the design of a blocking-transmission vaccine in which the use of liposomes, viral particles, and bacterial carriers has been used to deliver the selected antigen [27][28][29][30][31].…”

Section: Second-generation Vaccinesmentioning

confidence: 99%

“…For this, Salmonella pAg30 vaccine was engineered to express the Ag30 peptide from T. spiralis fused to three copies of P28 adjuvant (Ag30-P28 3 ) and was either expressed on the bacterial surface or secreted to the milieu [31]. Salmonella vaccines were administered to mice by i.n.…”

Section: Second-generation Vaccinesmentioning

confidence: 99%

Vaccination against Trichinella spiralis: Potential, Limitations and Future Directions

Andrade-Becerra¹,

Pompa-Mera²,

MaríaRibas-Aparicio³

et al. 2017

Natural Remedies in the Fight Against Parasites

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Trichinellosis is a food-borne parasitic disease caused by round worms of the genus Trichinella. The majority of human outbreaks are attributed to consumption of raw or undercooked pork meat contaminated with T. spiralis muscle larvae. A blockingtransmission vaccine against trichinellosis will allow preventing swine infection and will contribute to disease control. In this chapter, different vaccine candidates so far developed against T. spiralis, including first-, second-, and third-generation vaccines, are discussed. Most vaccine candidates are based on a unique antigen mainly from the muscle larva stage, inducing with some exceptions, partial protection although a mix Th1/Th2 immune response is elicited. Therefore, the need for identification of new antigens from different parasite stages focusing on infective intestinal larvae, adult, and newborn larvae stages as well as the evaluation of their protective capacity in pigs is presented. The design of multi-epitope vaccines and the use of adjuvants or immunomodulatory molecules capable to polarize the immune response to a Th2-type-protective response are discussed as imperative elements of modern vaccines. Plant-based vaccines and probiotics as excellent tools for vaccine development against T. spiralis are also presented as an attractive platform for veterinary vaccines.

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Protective immunity against enteral stages of Trichinella spiralis elicited in mice by live attenuated Salmonella vaccine that secretes a 30-mer parasite epitope fused to the molecular adjuvant C3d-P28

Cited by 37 publications

References 72 publications

Influence of adjuvant formulation on inducing immune response in mice immunized with a recombinant serpin from Trichinella spiralis

Influence of adjuvant formulation on inducing immune response in mice immunized with a recombinant serpin from Trichinella spiralis

Protective immunity in mice vaccinated with a novel elastase-1 significantly decreases Trichinella spiralis fecundity and infection

Vaccination against Trichinella spiralis: Potential, Limitations and Future Directions

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