Protective immunity against acute toxoplasmosis in BALB/c mice induced by a DNA vaccine encoding Toxoplasma gondii elongation factor 1-alpha

Wang, Shuai; Wang, YuJian; Sun, Xun; Zhang, Zhenchao; Liu, Tingqi; Gadahi, Javaid Ali; Hassan, Ibrahim A.; Xu, Lixin; Ren, Yan; Song, Xiaoling; Li, Xiangrui

doi:10.1186/s12879-015-1220-5

Cited by 12 publications

(7 citation statements)

References 69 publications

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“…This would be consistent with a mode of action that is relevant for invasion or early host cell establishment. In addition, vaccination of mice with recombinant TgEF1␣ and a DNA vaccine coding for TgEF1␣ led to significantly prolonged survival times in T. gondii-infected mice (74,75), underlining the importance of TgEF1␣ for the infection process.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Activities of Dinuclear Thiolato-Bridged Arene Ruthenium Complexes against Toxoplasma gondii

Basto

Müller

Rubbiani

et al. 2017

Antimicrob Agents Chemother

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The effects of 18 dinuclear thiolato-bridged arene ruthenium complexes (1 monohiolato compound, 4 dithiolato compounds, and 13 trithiolato compounds), originally designed as anticancer agents, on the apicomplexan parasite grown in human foreskin fibroblast (HFF) host cells were studied. Some trithiolato compounds exhibited antiparasitic efficacy at concentrations of 250 nM and below. Among those, complex 1 and complex 2 inhibited proliferation with 50% inhibitory concentrations (ICs) of 34 and 62 nM, respectively, and they did not affect HFFs at dosages of 200 μM or above, resulting in selectivity indices of >23,000. The ICs of complex 9 were 1.2 nM for and above 5 μM for HFFs. Transmission electron microscopy detected ultrastructural alterations in the matrix of the parasite mitochondria at the early stages of treatment, followed by a more pronounced destruction of tachyzoites. However, none of the three compounds applied at 250 nM for 15 days was parasiticidal. By affinity chromatography using complex 9 coupled to epoxy-activated Sepharose followed by mass spectrometry, translation elongation factor 1α and two ribosomal proteins, RPS18 and RPL27, were identified to be potential binding proteins. In conclusion, organometallic ruthenium complexes exhibit promising activities against , and the potential mechanisms of action of these compounds as well as their prospective applications for the treatment of toxoplasmosis are discussed.

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Section: Discussionmentioning

confidence: 99%

Characterization of the Activities of Dinuclear Thiolato-Bridged Arene Ruthenium Complexes against Toxoplasma gondii

Basto

Müller

Rubbiani

et al. 2017

Antimicrob Agents Chemother

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“…As such, splenic infections were strictly limited to capsular areas of the spleen in 10 tachyzoites infected mice, while spleens of mice infected with higher doses were subjected to parasitic infiltration through the splenic capsule. Poor vaccine efficacies were observed from previous studies using high doses of tachyzoites for vaccine challenge experiment [5][6][7][8][9][10][11][12], indicating different vaccine efficacies might be concluded if low dosage of tachyzoites were used. Since all mice died from 10, 30, and 100 tachyzoites infections after spleen infiltration and apoptosis from 30 and 100 tachyzoites infected mice post-infection, we conclude that 30 tachyzoites dosage can be used for vaccine or apoptosis study.…”

mentioning

confidence: 91%

“…Oocysts in the feces of definitive host can be ingested by other intermediate hosts, which results in infection. Mice as intermediate hosts are extensively used for pathogenesis and vaccine study of toxoplas-mosis, in which high numbers of tachyzoites of T. gondii (RH) (10 3 , 10 4 , 10 5 ) have been used [5][6][7][8][9][10][11][12]. T. gondii (RH) is highly virulent and its infection in mice causes death [13].…”

mentioning

confidence: 99%

“…Mice infected with 10 5 tachyzoites show no CD8 + T and germinal center B cell responses from the spleen at day 16 post-infection, since these immune cells are largely damaged [12]. Low vaccine efficacies were reported upon challenge infection with T. gondii (RH) at high dosage (10 3 , 10 4 , 10 5 ), in which mice died at very early stage of infection and immune responses elicited by challenge infection cannot be detected [5][6][7][8][9][10][11]. Thus, finding correct infection dosage for challenge infection to evaluate vaccine efficacy and immune responses is extremely important.…”

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confidence: 99%

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Parasite Infiltration and Apoptosis in Spleen upon Toxoplasma gondii Infection

2019

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Toxoplasma gondii infection induces parasite infiltration and apoptosis in the spleen. However, dose-dependent parasite infiltration, apoptosis, body weight alternations and survival in mice remain largely unknown. In this study, mice were intraperitoneally infected with 10, 30 or 100 tachyzoites of T. gondii, respectively. Parasite infiltration and apoptosis in the spleen were analyzed on days 3, 7, and 9 post-infection by immunohistochemistry and flow cytometry. Significantly higher levels of T. gondii infiltration and apoptosis in the spleen were found in 30 and 100 tachyzoites infected mice compared to 10 tachyzoites infected mice on days 7 and 9 post-infection. Although 30 and 100 tachyzoites infected mice showed significant body weight loss compared to 10 tachyzoites infected mice, all of the 100, 30, and 10 tachyzoites infected mice died by days 12, 15, and 17, each respectively. Interestingly, T. gondii infiltration in 10 tachyzoites infected mice were limited to capsule area of the spleen on day 9 post-infection. Several areas of parasite infiltrations were found in the 30 tachyzoites infected mice, where noticeable levels of splenic capsule de-adhesion occurred. These results indicated that parasite infiltration and apoptosis in the spleen, as well as body weight loss (survival) are closely correlated with infection dosage. The level of T. gondii infiltration and apoptosis in the spleen and splenic de-adhesion were dependent on the parasite dose.

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“…Toxoplasma gondii is an important protozoan parasite found worldwide that potentially infects all warm-blooded vertebrates, including mammals, birds, and humans [9, 19, 39]. Sheep are an important intermediate host of T. gondii [36].…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence ofToxoplasma gondiiinfection and risk factors in domestic sheep in Henan province, central China

Zhang¹,

Wang²,

Wang³

et al. 2016

Parasite

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Sheep are highly susceptible to infections with Toxoplasma gondii and play a major role in the transmission of toxoplasmosis to humans. In the present study, 779 serum samples from sheep were collected from Henan province, central China from March 2015 to May 2016, and antibodies to T. gondii were detected by modified agglutination test (MAT). The overall seroprevalence of T. gondii in sheep was 12.71% (99/779). The risk factors significantly associated with T. gondii seroprevalence were the geographical origin, age, presence of cats, and the rearing system. This is the first report of T. gondii infection in sheep in Henan province, central China, and of an association of seropositivity to T. gondii with risk factors.

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Protective immunity against acute toxoplasmosis in BALB/c mice induced by a DNA vaccine encoding Toxoplasma gondii elongation factor 1-alpha

Cited by 12 publications

References 69 publications

Characterization of the Activities of Dinuclear Thiolato-Bridged Arene Ruthenium Complexes against Toxoplasma gondii

Characterization of the Activities of Dinuclear Thiolato-Bridged Arene Ruthenium Complexes against Toxoplasma gondii

Parasite Infiltration and Apoptosis in Spleen upon Toxoplasma gondii Infection

Seroprevalence ofToxoplasma gondiiinfection and risk factors in domestic sheep in Henan province, central China

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