Protective humoral immunity in guinea pigs induced by PCV2 virus-like particles displaying the B cell linear epitope (228QQITDA233) of PPV1

Wang, Naidong; Zhang, Sujiao; Wang, Dongliang; Li, Fuqiang; Liang, Lin; Li, Xiuli; Zou, Yawen; Zhan, Yang; Chen, Guan-Yu; Yu, Wanting; Deng, Zhibang; Tu, Di; Cui, Shangjin

doi:10.1016/j.vetmic.2019.06.002

Cited by 1 publication

(2 citation statements)

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“…The lymphocyte proliferation response is also a good indicator of recovery from viral infection [ 31 ]; as such, the highest level of SI was detected in the VLP-JEVe group, followed by the group inoculated with the inactivated PPV vaccine. PPV mostly causes fetal and early neonatal mortality, and the tissue contents of PPV in infected guinea pigs are a good indication of the antiviral effects of any vaccine [ 45 ]. The genomic copy numbers of PPV in VLP-JEVe-inoculated guinea pigs were significantly lower than in the PBS control group, which clearly demonstrated that VLP-JEVe could serve to protect the animals from PPV virulent challenge.…”

Section: Discussionmentioning

confidence: 99%

“…The genomic copy numbers of PPV in VLP-JEVe-inoculated guinea pigs were significantly lower than in the PBS control group, which clearly demonstrated that VLP-JEVe could serve to protect the animals from PPV virulent challenge. According to these data, VLP-JEVe performs better than a PPV vaccine in guinea pigs and perhaps also for other existing PPV vaccines, which needs to be tested in the future [ 45 , 46 ].…”

Section: Discussionmentioning

confidence: 99%

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A Novel Recombinant Virus-Like Particles Displaying B and T Cell Epitopes of Japanese Encephalitis Virus Offers Protective Immunity in Mice and Guinea Pigs

Anwar

Jiang

et al. 2021

Vaccines

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Virus-like particles (VLPs) are non-replicative vectors for the delivery of heterologous epitopes and are considered one of the most potent inducers of cellular and humoral immune responses in mice and guinea pigs. In the present study, VLP-JEVe was constructed by the insertion of six Japanese encephalitis virus (JEV) envelope protein epitopes into different surface loop regions of PPV VP2 by the substitution of specific amino acid sequences without altering the assembly of the virus; subsequently, the protective efficacy of this VLP-JEVe was evaluated against JEV challenge in mice and guinea pigs. Mice immunized with the VLP-JEVe antigen developed high titers of neutralizing antibodies and 100% protection against lethal JEV challenge. The neutralizing and hemagglutination inhibition (HI) antibody responses were also induced in guinea pigs vaccinated with VLP-JEVe. In addition, immunization with VLP-JEVe in mice induced effective neutralizing antibodies and protective immunity against PPV (porcine parvovirus) challenge in guinea pigs. These studies suggest that VLP-JEVe produced as described here could be a potential candidate for vaccine development.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%