Protective efficacy of recombinant proteins AMA1 and IMP1 in rabbits infected with Eimeria intestinalis

Xiao, Jie; Wang, He; Xiong, Changming; Ge, Hao; Pu, Jiayan; Chen, Hao; Xu, Liwen; Zhu, Yazhou; Ren, Yongjun; Yang, Guangyou

doi:10.1016/j.vetpar.2023.109985

Cited by 4 publications

(2 citation statements)

References 51 publications

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“…The recombinant E. magna surface antigens rEm-SAG10 and rEm-SAG11 [29], the microneme proteins rEm-MIC2 and rEm-MIC3 [30], the gametophyte antigen rEm-GAM56 [31], and the rhoptry protein rEm-ROP17 [31] provided good immune protection after the immunization of the rabbits. There was only one study of a recombinant subunit vaccine against E. intestinalis, indicating that rEi-AMA1 and rEi-IMP1 provided moderate immune protection in rabbits, with ACI values of 152.09 and 147.17, respectively [17].…”

Section: Discussionmentioning

confidence: 99%

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Assessment of the Immunoprotective Efficacy of Recombinant 14-3-3 Protein and Dense Granule Protein 10 (GRA10) as Candidate Antigens for Rabbit Vaccines against Eimeria intestinalis

Xiong,

He,

Xiao

et al. 2023

IJMS

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Eimeria intestinalis infects rabbits, causing severe intestinal coccidiosis. Prolonged anticoccidial drug use might lead to coccidia resistance and drug residues in food. Thus, vaccines are required to control rabbit coccidiosis. In this study, recombinant E. intestinalis 14-3-3 and GRA10 proteins (rEi-14-3-3 and rEi-GRA10) were obtained via prokaryotic expression and used as recombinant subunit vaccines. Fifty 30-day-old rabbits were randomly grouped as follows: PBS-uninfected group, PBS-infected group, Trx-His-S control group, and rEi-14-3-3 and rEi-GRA10 immunized groups. The rabbits were subcutaneously immunized twice at 2-week intervals, challenged with 7 × 104 sporulated oocysts, and sacrificed 14 days later. The protective effects were assessed via clinical signs, relative weight gain, oocyst reduction, mean intestinal lesion score, ACI (anticoccidial index), cytokine, and specific antibody levels in sera. The rEi-14-3-3 and rEi-GRA10 groups had higher relative weight gain rates of 81.94% and 73.61% (p < 0.05), and higher oocyst reduction rates of 86.13% and 84.87% (p < 0.05), respectively. The two immunized groups had fewer intestinal lesions (p < 0.05) and higher IgG levels (p < 0.05). Higher levels of IL-2, IL-4, and IFN-γ cytokines in the rEi-14-3-3 group (p < 0.05) and a higher level of IFN-γ in the rEi-GRA10 group (p < 0.05) were observed. The ACI values of the rEi-14-3-3 and rEi-GRA10 groups were 168.24 and 159.91, with good and moderate protective effects, respectively. Both rEi-14-3-3 and rEi-GRA10 induced humoral immunity in the rabbits. In addition, rEi-14-3-3 induced Th1- and Th2-type immune responses. Both recombinant proteins were protective against E. intestinalis infection in rabbits, with rEi-14-3-3 showing a better protective effect.

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Section: Discussionmentioning

confidence: 99%

“…The identification and proper selection of protective antigens are crucial for recombinant subunit vaccine development [15,16]. To date, there has been only one report on recombinant subunit vaccines for E. intestinalis [17].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Immunoprotective Efficacy of Recombinant 14-3-3 Protein and Dense Granule Protein 10 (GRA10) as Candidate Antigens for Rabbit Vaccines against Eimeria intestinalis

Xiong,

He,

Xiao

et al. 2023

IJMS

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Evaluation of the immunoprotective effect of the recombinant Eimeria intestinalis rhoptry protein 25 and rhoptry protein 30 on New Zealand rabbits

Hao,

Xiong,

Xiao

et al. 2024

Experimental Parasitology

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Protection against Eimeria intestinalis infection in rabbits immunized with the recombinant elongation factors EF1α and EFG

He,

Hao,

Xiong

et al. 2023

Infect Immun

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Eimeria intestinalis is the most pathogenic species of rabbit coccidiosis, causing weight loss, diarrhea, and even acute death. The currently used anticoccidial drugs against E. intestinalis in rabbits are associated with drug resistance and residues. Immunological control might be a potential alternative. We cloned and expressed the E. intestinalis recombinant EF1α and EFG (r Ei -EF1α and r Ei -EFG, respectively). Rabbits were immunized subcutaneously every 14 days with 100 µg of r Ei -EF1α and r Ei -EFG and followed by 5 × 10 4 E. intestinalis sporulated oocysts orally challenge. Serum samples were collected every 7 days to measure the levels of specific antibodies and cytokines. On post-challenge day 14, rabbits were sacrificed and the anticoccidial index was evaluated. The rabbits of PBS challenged groups exhibited anorexia, diarrhea, marked intestinal wall thickening, and white nodules that formed patches, while rabbits from the r Ei -EF1α or r Ei -EFG challenged group exhibited milder symptoms. The r Ei -EF1α group showed a 75.18% oocyst reduction and 89.01%wt gain; the r Ei -EFG group had a 60.58% oocyst reduction and 56.04%wt gain. After vaccination, specific IgG levels increased and stayed high ( P < 0.05). The IL-4 and IL-2 levels of r Ei -EF1α immunized groups showed a significant increase after immunization ( P < 0.05). Both r Ei -EF1α and r Ei -EFG could induce humoral and cellular immune responses. In contrast, rabbits immunized with r Ei -EF1α were better protected from challenge by E. intestinalis than r Ei -EFG.

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Protective efficacy of recombinant proteins AMA1 and IMP1 in rabbits infected with Eimeria intestinalis

Cited by 4 publications

References 51 publications

Assessment of the Immunoprotective Efficacy of Recombinant 14-3-3 Protein and Dense Granule Protein 10 (GRA10) as Candidate Antigens for Rabbit Vaccines against Eimeria intestinalis

Assessment of the Immunoprotective Efficacy of Recombinant 14-3-3 Protein and Dense Granule Protein 10 (GRA10) as Candidate Antigens for Rabbit Vaccines against Eimeria intestinalis

Evaluation of the immunoprotective effect of the recombinant Eimeria intestinalis rhoptry protein 25 and rhoptry protein 30 on New Zealand rabbits

Protection against Eimeria intestinalis infection in rabbits immunized with the recombinant elongation factors EF1α and EFG

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