Protective efficacy of a DNA vaccine construct encoding the VP2 gene of infectious bursal disease and a truncated HSP70 of Mycobacterium tuberculosis in chickens

Maity, Hemanta Kumar; Dey, Sohini; Mohan, C. Madhan; Khulape, Sagar A.; Pathak, D. C.; Vakharia, Vikram N.

doi:10.1016/j.vaccine.2015.01.006

Cited by 24 publications

(14 citation statements)

References 37 publications

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“…However, MLVs are not fully efficacious against IBDV field challenges and induce moderate to severe bursal lesions and immunosuppression. The major capsid protein VP2 has proved to be an important target for generating cellular and humoral immune responses capable of conferring active and passive protection against IBDV infection [ 29 , 30 ]. There are several reports using recombinant live viral vectors including herpes virus of turkey [ 31 , 32 ], NDV [ 11 ], fowl pox virus [ 33 ], Marek’s disease virus [ 34 ] and avian adenovirus [ 35 ] as expression vectors incorporating VP2 with the aim of protecting birds against IBD.…”

Section: Discussionmentioning

confidence: 99%

Newcastle Disease Virus Vectored Bivalent Vaccine against Virulent Infectious Bursal Disease and Newcastle Disease of Chickens

et al. 2017

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Newcastle disease virus (NDV) strain F is a lentogenic vaccine strain used for primary vaccination in day-old chickens against Newcastle disease (ND) in India and Southeast Asian countries. Recombinant NDV-F virus and another recombinant NDV harboring the major capsid protein VP2 gene of a very virulent infectious bursal disease virus (IBDV); namely rNDV-F and rNDV-F/VP2, respectively, were generated using the NDV F strain. The rNDV-F/VP2 virus was slightly attenuated, as compared to the rNDV-F virus, as evidenced from the mean death time and intracerebral pathogenicity index analysis. This result indicates that rNDV-F/VP2 behaves as a lentogenic virus and it is stable even after 10 serial passages in embryonated chicken eggs. When chickens were vaccinated with the rNDV F/VP2, it induced both humoral and cell mediated immunity, and was able to confer complete protection against very virulent IBDV challenge and 80% protection against virulent NDV challenge. These results suggest that rNDV-F could be an effective and inherently safe vaccine vector. Here, we demonstrate that a bivalent NDV-IBDV vaccine candidate generated by reverse genetics method is safe, efficacious and cost-effective, which will greatly aid the poultry industry in developing countries.

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Section: Discussionmentioning

confidence: 99%

Newcastle Disease Virus Vectored Bivalent Vaccine against Virulent Infectious Bursal Disease and Newcastle Disease of Chickens

et al. 2017

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“…Infectious bursal disease virus (IBDV) is a birnavirus that causes Gumboro, an acute and highly contagious disease that destroys B lymphocytes within the bursa of Fabricius, causing morbidity, mortality and immunosuppression, leaving birds susceptible to other infections and compromising vaccination programmes [13]. The IBDV major capsid protein VP2 is the target of antiviral neutralising antibodies [14–16] and is already used in a licenced HVT-vectored vaccine (Vaxxitec, Merial). Infectious laryngotracheitis virus (ILTV) is an alphaherpesvirus that causes highly contagious acute respiratory disease leading to growth depression, reduced egg production and death [17].…”

Section: Introductionmentioning

confidence: 99%

Viral proteins expressed in the protozoan parasite Eimeria tenella are detected by the chicken immune system

et al. 2016

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BackgroundEimeria species are parasitic protozoa that cause coccidiosis, an intestinal disease commonly characterised by malabsorption, diarrhoea and haemorrhage that is particularly important in chickens. Vaccination against chicken coccidiosis is effective using wild-type or attenuated live parasite lines. The development of protocols to express foreign proteins in Eimeria species has opened up the possibility of using Eimeria live vaccines to deliver heterologous antigens and function as multivalent vaccine vectors that could protect chickens against a range of pathogens.ResultsIn this study, genetic complementation was used to express immunoprotective virus antigens in Eimeria tenella. Infectious bursal disease virus (IBDV) causes Gumboro, an immunosuppressive disease that affects productivity and can interfere with the efficacy of poultry vaccination programmes. Infectious laryngotracheitis virus (ILTV) causes a highly transmissible respiratory disease for which strong cellular immunity and antibody responses are required for effective vaccination. Genes encoding the VP2 protein from a very virulent strain of IBDV (vvVP2) and glycoprotein I from ILTV (gI) were cloned downstream of 5’Et-Actin or 5’Et-TIF promoter regions in plasmids that also contained a mCitrine fluorescent reporter cassette under control of the 5’Et-MIC1 promoter. The plasmids were introduced by nucleofection into E. tenella sporozoites, which were then used to infect chickens. Progeny oocysts were sorted by FACS and passaged several times in vivo until the proportion of fluorescent parasites in each transgenic population reached ~20 % and the number of transgene copies per parasite genome decreased to < 10. All populations were found to transcribe and express the transgene and induced the generation of low titre, transgene-specific antibodies when used to immunise chickens.ConclusionsE. tenella can express antigens of other poultry pathogens that are successfully recognised by the chicken immune system. Nonetheless, further work has to be done in order to improve the levels of expression for its future use as a multivalent vaccine vector.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1756-2) contains supplementary material, which is available to authorized users.

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“…IBD is an economically important, immunosuppressionrelated infectious disease of young chickens worldwide [26] . BC6/85 is a standard virulent IBDV strain and is widely used in China for evaluating the efficacy of IBDV vaccines [13] .…”

Section: Discussionmentioning

confidence: 99%

Oral administration of Allium sativum extract protects against infectious bursal disease in chickens

Zhao¹,

Jia²,

Zhang³

et al. 2015

Front. Agr. Sci. Eng.

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Garlic (Allium sativum, Liliaceae) has been safely used for more than 5000 years, and research on garlic extract is rapidly increasing because of its multiple biological functions. The in vivo effects of oral administration of garlic mixture (GM, water-soluble extract) on infectious bursal disease virus (IBDV)-infected specific pathogen free male white leghorn chicken were examined through histopathological, immunohistochemical, and Western blot analyses, and enzyme-linked immunosorbent assay. The results confirmed the protective effects of oral administration of 5 mg$kg -1 BW GM (Group GM1) on bursal lesions after IBDV infection. In particular, protein expression of IBDV in the bursa decreased in Group GM1, indicating that GM administration decreased IBDV replication in the bursa. Furthermore, immunoglobulin M-and A-bearing B lymphocytes significantly increased 7 days post infection in bursae in Group GM1 (P < 0.01), suggesting that the oral administration of 5 mg$kg -1 GM offers moderate protection against B cell destruction after IBDV infection. During infection, the concentration of bursal interferon gamma (IFN-g) increased and peaked in Group GM1 earlier than in Group T (IBDV-exposed), demonstrating that GM administration prompted the production of IFN-g to protect against IBDV infection.

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Protective efficacy of a DNA vaccine construct encoding the VP2 gene of infectious bursal disease and a truncated HSP70 of Mycobacterium tuberculosis in chickens

Cited by 24 publications

References 37 publications

Newcastle Disease Virus Vectored Bivalent Vaccine against Virulent Infectious Bursal Disease and Newcastle Disease of Chickens

Newcastle Disease Virus Vectored Bivalent Vaccine against Virulent Infectious Bursal Disease and Newcastle Disease of Chickens

Viral proteins expressed in the protozoan parasite Eimeria tenella are detected by the chicken immune system

Oral administration of Allium sativum extract protects against infectious bursal disease in chickens

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