Protective Effects of Zinc, Pentoxifylline, and N-Acetylcysteine in an Animal Model of Laparoscopy-Induced Ischemia/Reperfusion Injury of the Small Intestine

Bülbüloğlu, Ertan; Yıldız, Hüseyin Bekir; Şenoğlu, Nimet; Çoşkuner, İsmail; Yüzbaşıoğlu, Mehmet Fatih; Kılınç, Metin; Doğan, Zafer; Deniz, Caglayan; Öksüz, Hafize; Kantarçeken, Bülent; Atlı, Yalçın

doi:10.1089/lap.2011.0194

Cited by 14 publications

(13 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Interestingly, by comparison of eIV and controls only 50 genes were found to be differentially regulated and found to be associated with down-regulation of zinc ion binding in early IV. It is well known that zinc plays an important role in ischemia/reperfusion injury and has potential antioxidant properties [32]. Recent work shows that oral zinc chloride supplementation mitigates renal ischemia/reperfusion injury in rats by modulating oxidative stress, endoplasmic reticulum stress, and autophagy [33].…”

Section: Discussionmentioning

confidence: 99%

Early isolated V-lesion may not truly represent rejection of the kidney allograft

et al. 2018

View full text Add to dashboard Cite

Intimal arteritis is known to be a negative prognostic factor for kidney allograft survival. Isolated v-lesion (IV) is defined as intimal arteritis with minimal tubulointerstitial inflammation (TI). Although the Banff classification assesses IV as T cell-mediated rejection (TCMR), clinical, and prognostic significance of early IV (early IV, eIV) with negative C4d and donor-specific antibodies (DSA) remains unclear. To help resolve if such eIV truly represents acute rejection, a molecular study was performed. The transcriptome of eIV (n=6), T cell-mediated vascular rejection with rich TI (T cell-mediated vascular rejection, TCMRV, n=4) and non-rejection histologic findings (n=8) was compared using microarrays. A total of 310 genes were identified to be deregulated in TCMRV compared with eIV. Gene enrichment analysis categorized deregulated genes to be associated primarily with T-cells associated biological processes involved in an innate and adaptive immune and inflammatory response. Comparison of deregulated gene lists between the study groups and controls showed only a 1.7% gene overlap. Unsupervised hierarchical cluster analysis revealed clear distinction of eIV from TCMRV and showed similarity with a control group. Up-regulation of immune response genes in TCMRV was validated using RT-qPCR in a different set of eIV (n=12) and TCMRV (n=8) samples. The transcriptome of early IV (< 1 month) with negative C4d and DSA is associated with a weak immune signature compared with TCMRV and shows similarity with normal findings. Such eIV may feature non-rejection origin and reflect an injury distinct from an alloimmune response. The present study supports use of molecular methods when interpreting kidney allograft biopsy findings.

show abstract

Section: Discussionmentioning

confidence: 99%

Early isolated V-lesion may not truly represent rejection of the kidney allograft

et al. 2018

View full text Add to dashboard Cite

show abstract

“…It degrades hydrogen peroxide into water and oxygen [20]. Bulbuloglu et al reported in an experimental study that CAT activity decrease in I/R injury secondary to Pp and increases after administration of antioxidant [38]. An experimental model of I/R injury of muscle tissue which assess antioxidant activities with DEX administration, CAT activity in I/R injury group was found lower, on the other hand CAT activity in DEX administration group before reperfusion was higher than control group.…”

Section: Resultsmentioning

confidence: 99%

The effects of Dexmedetomidine on oxidant - antioxidant systems in the experimental model of carbondioxide pneumoperitoneum

Taş¹

2014

Turk J Bioch

View full text Add to dashboard Cite

Objective: The aim of the study was to investigate the changes of oxidative and anti-oxidative systems in the splanchnic area during carbon dioxide pneumoperitoneum and to determine whether the administration of dexmedetomidine has effects on these systems. Methods: Forty rats were randomized into four groups: Group I; Control, Group II; No pneumoperitoneum, Dexmedetomidine administration, Group III; Pneumoperitoneum, no Dexmedetomidine administration and Group IV; Pneumoperitoneum and Dexmedetomidine administration 30 minutes before insufflation. The rats were rested 30 minutes after desufflation and blood samples were obtained for; ischaemia modified albumin (IMA), myeloperoxidase (MPO), advanced oxidation protein products (AOPP), catalase (CAT), paraoxonase (PON1) and platelet-activating factor acetylhydrolase (PAF-AH) analyses. Results: When compared with the control group; the serum IMA levels significantly decreased in group II, and also increased in group III as compared to control (p<0.05). IMA levels were also significantly decreased in both groups II and IV as compared to group III (p<0.001). Serum MPO activity increased in group III as compared to control (p<0.05). Serum AOPP levels were significantly increased in group III as compared to group II (p<0.01) and decreased in group IV as compared to group III (p<0.01). Serum CAT activity was higher in group II than controls (p<0.05). Serum PON and plasma PAF-AH activities significantly decreased in grup III as compared to group II (p<0.05) and plasma PAF-AH activity were decreased in group III as compared to controls (p<0.05). Conclusion:In conclusion, administration of dexmedetomidine; prior to ischemia reperfusion injury caused by pneumoperitoneum; reduces the oxidative injury and increases the antioxidant activity in the acute period.

show abstract

“…They show that these inhibitor effects by both enabling the cleaning of free radicals from the environment and inhibiting xanthine oxidase and NADPH oxidase activities. Many studies have shown that zinc can prevent I/R damage in tissues with this mechanism, including on the myocardium (Xu & Zhou, ), kidney (Guo et al., ), small intestine (Bulbuloglu et al., ), retina (Ophir, Berenshtein, Kitrossky, & Averbukh, ), and gastric mucosa (Yoshikawa et al., ). Furthermore, as previously mentioned, zinc is also known to be an important regulator responsible for intracellular signal transmission and shows this effect through receptors, protein kinase and transcriptional factors (Beyersmann & Haase, ).…”

Section: Discussionmentioning

confidence: 99%

“…After the surgical procedures, approximately 100 mg of ground tissue (Un et al., ) was homogenised in 1 ml of phosphate‐buffered saline homogenate buffer in an Eppendorf tube using tissue lyser II and then centrifuged. SOD (Sun, Oberley, & Li, ), GSH (Sedlak & Lindsay, ) and MDA (Ohkawa, Ohishi, & Yagi, ) levels from each sample supernatant and standards were measured at room temperature in duplicate according to the modified methods of the ELISA reader, as described previously (Bulbuloglu et al., ).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of oral zinc administration on long-term ipsilateral and contralateral testes damage after experimental testis ischaemia-reperfusion

et al. 2016

View full text Add to dashboard Cite

The purpose of this study was to examine potential long-term post-torsion changes that can occur in the histopathology, biochemistry and spermatogenesis of both torsioned and nontorsioned opposite testes. The study also determines the effect of zinc (Zn) administration on the testicular torsion/detorsion (T/D) damage on both testes. Forty-eight male rats, divided equally into eight groups: (SHAM), (SHAM+,Zn+), (T/D+, Zn- 1 month), (T/D+,Zn- 2 months), (T/D+,Zn- 3 months), (T/D+,Zn+ 1 months), (T/D+,Zn+ 2 months), (T/D+,Zn+ 3 months), have been used. Drug administration was carried out by adding 100 μg (0.016 ml/rat) Zn per rat to drinking water in related groups. Testicular damage decreased superoxide dismutase (SOD) and glutathione (GSH) and increased malondialdehyde (MDA) in the testis tissues of rats, while Zn administration increased SOD and GSH and decreased MDA in the testis tissues in comparison with the SHAM group. The beneficial effect of zinc sulphate was more evident on the nonrotated testis than the rotated testis. In the histopathological study, a significant decrease in torsion and detorsion injuries was observed in the treatment groups compared to the torsion and detorsion groups. We found a protective effect of zinc sulphate on oxidative stress as a result of T/D injuries in rats, especially for the nonrotated testis; results were supported histopathologically.

show abstract

Protective Effects of Zinc, Pentoxifylline, and N-Acetylcysteine in an Animal Model of Laparoscopy-Induced Ischemia/Reperfusion Injury of the Small Intestine

Cited by 14 publications

References 28 publications

Early isolated V-lesion may not truly represent rejection of the kidney allograft

Early isolated V-lesion may not truly represent rejection of the kidney allograft

The effects of Dexmedetomidine on oxidant - antioxidant systems in the experimental model of carbondioxide pneumoperitoneum

Effects of oral zinc administration on long-term ipsilateral and contralateral testes damage after experimental testis ischaemia-reperfusion

Contact Info

Product

Resources

About