Protective Effects of Zinc on 2.45 GHz Electromagnetic Radiation-Induced Oxidative Stress and Apoptosis in HEK293 Cells

Özsobacı, Nural Pastacı; Ergün, Dilek Düzgün; Tunçdemi̇r, Matem; Özçelik, Derviş

doi:10.1007/s12011-019-01811-6

Cited by 10 publications

(6 citation statements)

References 39 publications

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“…In the present study, acute exposure to 3500 MHz RF-EMR at an SAR of 2, 4, or 10 W/kg resulted in intensity-dependent increases in free radical load, which subsequently induced oxidative stress in the ACx and weakened the antioxidant defense system. These changes are consistent with previous observations in various cells and tissues [Esmekaya et al, 2011;Salah et al, 2013;Özsobacı et al, 2019]. Additionally, oxidative stress induced by 3500 MHz RF-EMR led to ultrastructural changes in the ACx.…”

Section: Discussionsupporting

confidence: 92%

Effects of Acute Exposure to 3500 MHz (5G) Radiofrequency Electromagnetic Radiation on Anxiety‐Like Behavior and the Auditory Cortex in Guinea Pigs

Yang

Zhang

et al. 2022

Bioelectromagnetics

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Numerous studies have shown that radiofrequency electromagnetic radiation (RF‐EMR) may negatively affect human health. We detected the effect of 3500 MHz RF‐EMR on anxiety‐like behavior and the auditory cortex (ACx) in guinea pigs. Forty male guinea pigs were randomly divided into four groups and exposed to a continuous wave of 3500 MHz RF‐EMF at an average specific absorption rate (SAR) of 0, 2, 4, or 10 W/kg for 72 h. After exposure, malondialdehyde (MDA) levels, antioxidant enzyme activity, anxiety‐like behavior, hearing thresholds, cell ultrastructure, and apoptosis were detected. Our results revealed that hearing thresholds and basic indexes of animal behavior did not change significantly after exposure (P > 0.05). However, the MDA levels of ACx were increased (P < 0.05), and catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH‐px) activities were decreased (P < 0.05) in the exposure groups compared to the sham group. Ultrastructural changes of ACx, including swollen mitochondria and layered myelin sheaths, were observed. Cytochrome‐c relocalization, caspase‐9, and cleaved caspase‐3 activation were detected in the exposure groups. In conclusion, these results suggest that oxidative stress is an important mechanism underlying the biological effects of RF‐EMR, which can induce ultrastructural damage to the ACx and cell apoptosis through a mitochondria‐dependent mechanism. Moreover, oxidative stress, apoptosis induction and ultrastructural damage increase in a SAR‐dependent manner. However, RF‐EMR does not increase hearing thresholds or induce anxiety. Bioelectromagnetics. 43:106–118, 2022. © 2021 Bioelectromagnetics Society.

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Section: Discussionsupporting

confidence: 92%

Effects of Acute Exposure to 3500 MHz (5G) Radiofrequency Electromagnetic Radiation on Anxiety‐Like Behavior and the Auditory Cortex in Guinea Pigs

Yang

Zhang

et al. 2022

Bioelectromagnetics

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“…Apoptosis and more ROS was also seen in MCF-7 breast cancer cells after exposure to 217 Hz pulsed 900, 1800, and 2450 MHz RF-EMF (average SAR: 0.36 W/kg) for 1 h [111]. RF-EMF-induced cell death was also observed in embryonic kidney cells (HEK293, HEK293T) exposed to an unmodulated 940 MHz carrier wave (SAR: 90 mW/kg) [218] or 2.45 GHz (E-field: 2 V/m) [219,220] for about 1 h. However, the results from the analyses of the markers for oxidative stress differed. While the 2.45 GHz RF-EMF resulted in higher MDA levels and reduced activities of SOD and GPx, exposure to the 940 MHz RF-EMF decreased MDA values over time while increasing SOD, with maximal changes observed at 30-45 min after the exposure started.…”

Section: Experimental Data On the Effect Of Emf On Skin Epithelial And Cancer Cellsmentioning

confidence: 98%

Manmade Electromagnetic Fields and Oxidative Stress—Biological Effects and Consequences for Health

Schuermann

Mevissen

2021

IJMS

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Concomitant with the ever-expanding use of electrical appliances and mobile communication systems, public and occupational exposure to electromagnetic fields (EMF) in the extremely-low-frequency and radiofrequency range has become a widely debated environmental risk factor for health. Radiofrequency (RF) EMF and extremely-low-frequency (ELF) MF have been classified as possibly carcinogenic to humans (Group 2B) by the International Agency for Research on Cancer (IARC). The production of reactive oxygen species (ROS), potentially leading to cellular or systemic oxidative stress, was frequently found to be influenced by EMF exposure in animals and cells. In this review, we summarize key experimental findings on oxidative stress related to EMF exposure from animal and cell studies of the last decade. The observations are discussed in the context of molecular mechanisms and functionalities relevant to health such as neurological function, genome stability, immune response, and reproduction. Most animal and many cell studies showed increased oxidative stress caused by RF-EMF and ELF-MF. In order to estimate the risk for human health by manmade exposure, experimental studies in humans and epidemiological studies need to be considered as well.

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“…Despite that, GSM-EMR exposure during the embryonic developmental period may induce an increase in the hepatic oxidative stress status at a speci c embryonic age in rats due to the decrease in the cytosolic and mitochondrial SOD activity, as well as, catalase expression and activity, resulting in an increase in the MDA lipid peroxidation product level. The inevitable exposure to EMR radiations was shown to make changes at the level of gene expression, and increase apoptosis in different kinds of mammalian embryonic cells and tissues [41][42][43][44]. Exposure to these radiations was also to deregulate the cell cycle [45,46], and lead to a chronic in ammation status that may result in different chronic disorders [47][48][49].…”

Section: Discussionmentioning

confidence: 99%

Impact of GSM-EMW exposure on the markers of oxidative stress in fetal rat liver

Salameh

Zeitoun-Ghandour

Sabra

et al. 2023

Preprint

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The current study investigated the effects of 24 hours/day prenatal exposure to global system for mobile communication electromagnetic fields (GSM-EMFs), 900 MHZ-induced electromagnetic radiation (EMR), on oxidative stress (OS) status, apoptotic, and inflammatory changes in liver of rats during their fetal development period. Fifty-two Sprague-Dawley pregnant rats were equally divided into control and exposed groups. Whole embryos were removed at 7.5 dpc (days post coitus), while liver tissues were extracted from embryos at 11.5, 15.5, and 19.5 dpc. For exposed animals, results showed an increased OS reflected by high levels of malondialdehyde (MDA), a decrease in cytosolic superoxide dismutase (cytoSOD) activity, in mitochondrial superoxide dismutase (mitoSOD) levels and catalase (CAT) mRNA expression but also in hepatic nuclear factor erythroïd 2-related Factor 2 (Nrf-2), protein kinase B (Akt1), and intercellular adhesion molecule-1 (ICAM-1) mRNA expression at 15.5 dpc. Moreover, GSM-EMR exposure was shown to significantly decrease mitoSOD and CAT activities at almost all studied ages. Thus, rat embryos may be protected by their mothers from OS, apoptotic, and pro-inflammatory responses till a sensitive developmental stage, during a continuous prenatal EMR exposure. This protection could be then created from the embryos themselves.

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Protective Effects of Zinc on 2.45 GHz Electromagnetic Radiation-Induced Oxidative Stress and Apoptosis in HEK293 Cells

Cited by 10 publications

References 39 publications

Effects of Acute Exposure to 3500 MHz (5G) Radiofrequency Electromagnetic Radiation on Anxiety‐Like Behavior and the Auditory Cortex in Guinea Pigs

Effects of Acute Exposure to 3500 MHz (5G) Radiofrequency Electromagnetic Radiation on Anxiety‐Like Behavior and the Auditory Cortex in Guinea Pigs

Manmade Electromagnetic Fields and Oxidative Stress—Biological Effects and Consequences for Health

Impact of GSM-EMW exposure on the markers of oxidative stress in fetal rat liver

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