Protective effects of Yerba mate (IIex paraguariensis) on prostate cancer development

Santiano, Flavia; Fernández, María de los Ángeles; Espino, Magdalena; Zyla, Leila; Rey, Lara; Gómez, Susana; Bruna, Flavia; Pistone-Creydt, Virginia; Pietrobon, Elisa Olivia; Elizalde, Rafael Pérez; Silva, María Fernanda; Carón, Rubén W.; Fontana, Constanza Matilde López

doi:10.1016/j.nut.2022.111957

Cited by 3 publications

(1 citation statement)

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“…Cell proliferation assay was conducted following a protocol already reported 52,53 . Non-tumorigenic (5x10 3 MCF-10A cells/well) and tumorigenic (2x10 3 MCF-7 or MDA-MB-231 cells/well) breast epithelial cell lines were seed into 96-well plates with DMEM-F12 supplemented with 10% of FBS for 24 h. Then, after treatment with 50% tumor or non-tumor MAT-CMs of HypoT or EUT or M199 and 50% DMEM-F12 medium with 2% of FBS for another 24 h, the cell proliferation was detected using a commercial colorimetric kit (Cell titer 96 AQueous One Solution Cell Proliferation Assay, MTS).…”

Section: Cell Proliferation Assaymentioning

confidence: 99%

Hypothyroidism and Mammary Cancer: Role of Peritumoral Adipose Tissue

Zyla

Santiano

Bruna

et al. 2023

Preprint

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To study the effects of HypoT on rat mammary tumor-associated adipose tissue and its ability to modify the biological behavior of breast cancer epithelial cells, we evaluated changes in the viability, proliferation, apoptosis, adhesion, and migration of tumorigenic (MCF-7, MDA-MB-231) and non-tumorigenic (MCF-10A) mammary cells incubated with the conditioned media from mammary adipose tissue (MAT-CMs) of HypoT and euthyroid (EUT) rats with and without mammary tumors. Female Sprague-Dawley rats were treated with 7, 12-Dimethylbenz[a]anthracen (15mg/rat) at 55 days of age to induce mammary tumors and were divided at random in HypoT (0.01% 6-N-propyl-2-thiouracil in drinking water, n = 30) and EUT (tap water, n = 30). Fragments of MAT were incubated for 24 h with M199 medium, and MAT-CMs were collected. MCF-7, MDA-MB-231, and MCF-10A were incubated with non-tumor and tumor MAT-CMs, and viability, proliferation, apoptosis, adhesion, and migration were quantified. Non-tumor MAT-CMs of HypoT rats favored apoptosis of MCF-10A; decreased the viability and adhesion of MCF-7; promoted proliferation, and decreased the adhesion of MDA-MB-231. Tumor MAT-CMs of HypoT rats stimulated proliferation in tumorigenic cells and inhibited apoptosis of MCF-10A. Non-tumor MAT-CMs of HypoT rats may protect against tumorigenesis while tumor MAT-CMs of HypoT may favor a more tumorigenic behavior of mammary tumor cells.

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Section: Cell Proliferation Assaymentioning

confidence: 99%