Protective effects of whey protein mixed with Garcinia kola and olive leaves extract against alloxan-induced oxidative stress and diabetes in rats

Abstract

Background: Manipulation of functional dairy products and natural phytochemicals may be a good source of micronutrients for traditional therapies and improve the hypoglycemia. The objective of this study to find out the protective effects of whey protein (WP) mixed with Garcinia kola seeds (GK) and extract of olive leaves (OL) against alloxan-induced oxidative stress and diabetes in rats. Methods: The 42 male Sprague-Dawley rats (120-150 g) were housed individually and randomly allocated to two main groups; normal group (n = 6) and six equal alloxan-induced diabetic groups (n = 36). Normal (first) and diabetic control (second) groups received basal diet only during the experiment, while groups (third, fourth, fifth, sixth, and seventh) received basal diet and an oral extract from WB (300 mg/day); OL (200 mg/day); WP and OL mixture (300 + 200 mg/day); GK (15 mg/day); and WP and GK mixture (300 + 15 mg/day) respectively. Biochemical markers including hematological parameters, glucose, BUN, creatinine, albumin, total protein, and liver enzymes were determined. Brain sample were taken for histopathological examination. Results: In comparison with second group, the administration of WP, GK, OL, WP and GK mixture, and WP and OL extracts respectively resulted in significant decrease in blood glucose (61.0 ± 10.8, 68.5 ± 6.6, 64.8 ± 14.6, 82.2 ± 8.4, and 91.7 ± 20 vs. 135.6 mg/dl ± 12.3 respectively). Liver enzymes were improved with administration of WP, GK, and OL extracts compared with positive control. Brain histopathological investigation showed reduction in tissue changes among rats received the suggested interventions. Conclusions: The obtained data can be concluded that administration of WP, GK, and OL extracts had evident favorable effects on blood glucose, major hematological, and biochemical parameters as well as the histological picture of brain.

Hypoglycemic, antidyslipidemic, and antioxidant activities of methanol extract of Struchium sparganophora leaves in alloxan-induced oxidative stress-mediated diabetes in rats

“…The extract, either administered in combination or alone, increased the SOD and GST activities in the liver and pancreas, while it also increases catalase activities in the liver and kidney. Some therapeutically promising antidiabetic medicinal plants and plant products have been documented with similar antioxidant efficacy [35][36][37][38].…”