Protective effects of Weipixiao decoction against MNNG-induced gastric precancerous lesions in rats

Cai, Tao; Zhang, Chengzhe; Zeng, Xiaohui; Zhao, Ziming; Yan, Yan; Yu, Xin; Wu, Lei; Lin, Lin; Pan, Huafeng

doi:10.1016/j.biopha.2019.109427

Cited by 23 publications

(16 citation statements)

References 38 publications

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“…These results were consistent with the findings of the present study. Also, the glycolysis pathway described in this study was reported in the results of a study by Cai, Zhang, et al, (2019).…”

Section: Discussionsupporting

confidence: 76%

Proteomics analyses of Xiaopi granules in N‐methyl‐N′‐nitro‐N‐nitrosoguanidine–induced gastric epithelial dysplasia rat model using LC–MS

Peng

Chen

Liang

et al. 2022

Biomedical Chromatography

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Xiaopi granules have been shown to ameliorate gastric epithelial dysplasia in patients.However, the therapeutic mechanism is unclear. Herein, the proteomics method was applied to identify the differentially expressed proteins and related pathways. Sixty male Sprague-Dawley rats were randomly divided into four groups: control (C group, n = 10), model (M group), Xiaopi granules (X group), and vitacoenzyme (V group). The rat gastric epithelial dysplasia model was established by intragastrically administering N-methyl-N 0 -nitro-N-nitrosoguanidine and ranitidine and by orally administering 0.05% ammonia solution. After 12 weeks, the stomach tissue was analyzed by hematoxylin and eosin staining and proteomics analyses. Western-blot analysis was applied to further validate the proteomics results. Compared to the M group, levels of 326 and 350 proteins were altered significantly in the X and V groups (1.5-fold, p < 0.05), which were significantly enriched in digestion, metabolism, coagulation, and cell apoptosis. CELA2A, GHRL, NDUFB9, and PGC were significantly upregulated (p < 0.0001), whereas CLCA1, PLG, and DAC2 were downregulated (p < 0.001 or 0.0001) in the M group vs. the C group. The change in these proteins could be reversed after the treatment of Xiaopi granules or vitacoenzyme tablets. Xiaopi granules ameliorated gastric epithelial dysplasia by intervening in digestion, metabolism, blood coagulation, cell apoptosis, and other related pathways.

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Section: Discussionsupporting

confidence: 76%

Proteomics analyses of Xiaopi granules in N‐methyl‐N′‐nitro‐N‐nitrosoguanidine–induced gastric epithelial dysplasia rat model using LC–MS

Peng

Chen

Liang

et al. 2022

Biomedical Chromatography

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“…The N group had free access to clean water and normal diet. This CAG rat model was generated by administration of MNNG (200 μg/ml) combined with irregular fasting (1 day fasting and 1 day feeding), and 2% sodium salicylate was intragastrically administered once per fasting day on an empty stomach, as described previously [ 12 , 13 , 17 ].…”

Section: Methodsmentioning

confidence: 99%

“…However, these treatments are often limited in clinical application and may have adverse effects. In China, many herbs based on traditional Chinese medicine (TCM) have been utilized to protect the gastric mucosa and reverse CAG [ 12 , 13 ]. Hua-Zhuo-Jie-Du (HZJD) is a Chinese formula that is widely used for treating CAG that is based on the TCM theories of detoxicating and resolving dampness.…”

Section: Introductionmentioning

confidence: 99%

16S rRNA sequencing-based evaluation of the protective effects of Hua-Zhuo-Jie-Du on rats with chronic atrophic gastritis

Zhou

Yang

Xu³

et al. 2022

BMC Complement Med Ther

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Background Disturbance of the intestinal flora is a pathogenic factor for chronic atrophic gastritis (CAG). Hua-Zhuo-Jie-Du (HZJD) has been shown to be an effective Chinese herbal preparation for treating CAG. However, the effects of HZJD on the intestinal flora of CAG is unclear. In this study, we probed the regulating effects of HZJD on intestinal microbes in CAG rats using 16S rRNA gene sequencing. Methods High-performance liquid chromatography (HPLC) analysis was used to perform quality control of HZJD preparations. We then administered 1-methyl-3-nitro-1-nitrosoguanidine (200 μg/ml) to Sprague–Dawley rats to establish a CAG model. HZJD and vitacoenzyme were administered orally to these rats over a 10 week period. Hematoxylin and eosin (H&E) staining was performed to observe the histopathology of CAG rats. A rarefaction curve, species accumulation curve, Chao1 index, and ACE index were calculated to assess the alpha diversity. Principal component analysis (PCA), non-metric multi-dimensional scaling (NMDS), and unweighted pair group method with arithmetic mean (UPGMA) were conducted to examine the beta diversity. The LEfSe method was used to identify differential bacteria. Differential function analysis used PCA based on KEGG function prediction. Results HPLC showed that our HZJD preparation method was feasible. H&E staining showed that HZJD significantly improved the pathological state of the gastric mucosa in CAG rats. The rarefaction curve and species accumulation curve showed that the sequencing data were reasonable. The Chao1 and ACE indices were significantly increased in CAG rats compared to the N group. Following HZJD and vitacoenzyme treatment, the Chao1 and ACE indices were decreased. PCA, NMDS, and UPGMA results showed that the M group was separated from the N, HZJD, and V groups, and LEfSe results showed that the relative abundance of Akkermansia, Oscillospira, Prevotella, and CF231 were significantly higher in the N group. Proteobacteria and Escherichia were significantly enriched in the M group, Allobaculum, Bacteroides, Jeotgalicoccus, Corynebacterium, and Sporosarcina were significantly enriched in the V group, and Firmicutes, Lactobacillus, and Turicibacter were significantly enriched in the HZJD group. Conclusion HZJD exhibited a therapeutic effect on the intestinal flora of CAG rats.

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“…For example, Xiao Tan He Wei decoction reverses PLGC by inducing apoptosis via the NF- κ B pathway [ 21 ]. Wei Pi Xiao could attenuate early angiogenesis by inhibiting the expression of angiogenesis-mediating VEGF, a downstream target of the HIF-1 α pathway [ 22 , 23 ]. In addition, epigallocatechin-3-gallate (EGCG) and astragaloside IV had a preventive effect on the N-methyl-N′-nitro-N-nitrosoguanidine- (MNNG-) induced gastrointestinal cancer [ 24 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq Analysis Reveals Dendrobium officinale Polysaccharides Inhibit Precancerous Lesions of Gastric Cancer through PER3 and AQP4

Zhao

Huang

Tang

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Purpose. There has been mounting evidence that Dendrobium officinale polysaccharides (DOP), a traditional Chinese medicine, are a potential candidate treatment for N-methyl-N′-nitro-N-nitrosoguanidine- (MNNG-) induced precancerous lesions of gastric cancer (PLGC). However, the underlying mechanisms have not been adequately addressed. Method. We utilized RNA-Seq analysis to investigate possible molecular targets and then used Venn software to identify the differentially expressed genes (DEGs). Further, we analyzed these DEGs with core analysis, upstream analysis, and interaction network analysis by IPA software and validated the DEGs by real-time PCR and Western blot. Result. 78 DEGs were identified from the normal control group (CON), the PLGC model group (MOD), and the DOP-treated group (DOP) by the Venn software. Further analysis of these DEGs, including core analysis, upstream analysis, and interaction network analysis, was performed by Ingenuity Pathway Analysis (IPA). The main canonical pathways involved were SPINK1 Pancreatic Cancer Pathway (−log ( P value) = 4.45, ratio = 0.0667) and Circadian Rhythm Signaling (−log ( P value) = 2.33, ratio = 0.0606). Circadian Rhythm Signaling was strongly upregulated in the model group versus the DOP group. CLOCK was predicted to be strongly activated (z-score = 2.236) in upstream analysis and induced the downstream PER3. In addition, the relative mRNA expression levels of seven DEGs (CD2AP, ECM1, AQP4, PER3, CMTM4, ESRRG, and KCNJ15) from RT-PCR agreed with RNA-Seq data from MOD versus CON and MOD versus DOP groups. The gene and protein expression levels of PER3 and AQP4 were significantly downregulated in the PLGC model and significantly increased by DOP treatment (9.6 g/kg). Conclusions. These findings not only showed DOP inhibits PLGC development by upregulating the PER3 and AQP4 gene and protein expression but also suggested that its mechanism of action involved modulating the Circadian Rhythm Signaling pathway.

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Protective effects of Weipixiao decoction against MNNG-induced gastric precancerous lesions in rats

Cited by 23 publications

References 38 publications

Proteomics analyses of Xiaopi granules in N‐methyl‐N′‐nitro‐N‐nitrosoguanidine–induced gastric epithelial dysplasia rat model using LC–MS

Proteomics analyses of Xiaopi granules in N‐methyl‐N′‐nitro‐N‐nitrosoguanidine–induced gastric epithelial dysplasia rat model using LC–MS

16S rRNA sequencing-based evaluation of the protective effects of Hua-Zhuo-Jie-Du on rats with chronic atrophic gastritis

RNA-Seq Analysis Reveals Dendrobium officinale Polysaccharides Inhibit Precancerous Lesions of Gastric Cancer through PER3 and AQP4

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