Protective Effects of Verapamil on Ischemia-Induced Hepatic Damage in the Rat

Abstract

The preventive effects of the calcium channel blocker, verapamil, on ischemic liver damage were studied using a rat total hepatic ischemic model. A marked improvement in the survival was obtained by verapamil administration. Following 90 min of hepatic ichemia, 8 of 9 rats (89%) survived in the verapamil-treated group compared to only a 50% survival rate in the saline-treated control group. Furthermore, 56% of the rats still survived after 120 min of ischemia, while there was no survivor in the control group. The recovery of hepatic ATP level following ischemia was significant in the verapamil-treated group, showing the well-preserved mitochondrial function afforded by verapamil administration.

The Effect of Dexmedetomidine and Propofol on Oxidative Stress and Antioxidizing System Studied on Liver Ischemia-reperfusion Model on Rats

“…The tissue damage that starts with the chemical reactions during the ischemia phase increases significantly with reoxygenation during the reperfusion phase [2]. Hepatocytes can stand against ischemia for 30-60 minutes.…”

“…The significance of using antioxidising agents such as ATP-MgCl 2 , coenzyme Q10, superoxide dismutase (SOD), catalase, prostaglandins, verapamil, alpha tocoferol(vitamin E), pentoxyphiline and N-acetyl cysteine is increasing to prevent ischemia reperfusion injury produced during surgical procedures either on liver tissue or other organ systems [2]. Propofol is one of the intravenous agents searched for its antioxidising effect.…”

“…Ischemia reperfusion injury is one of the major problems faced by all tissues during inadequate oxygenation [2]. On ischemic period decreased production of energy in the cell, rapid break down of the stored ATP and the icrease in the anaerobic glycolysis trigger mitochondrial disfunction, free oxygen radicals produced by reperfusion gives the actual major damage.…”

The Role of Endothelin inEarly Renal Cortical Reperfusionin Renal Transplantation

“…It is unlikely to be due to differences in trauma during transplantation since the animals in all the groups were operated nonsequentially by the same operator, had a similar range of rewarming ischaemia times (25-35 min) and survived the perioperative phase of the kidney transplantation. This phenomenon whereby only a proportion of the treated subjects respond to a given treatment has been reported in different experimental conditions including renal transplantation [29][30][31][32]. In the nonsurvivors, it may well be that the degree of I/R injury was too severe to allow for adequate early reperfusion.…”

Inhibition of Calcium Influx during Hypoxia/Reoxygenation in Primary Cultured Rat Hepatocytes

“…Calcium influx inhibition can be performed either by blocking sodium/proton exchanger or by inhibiting ROCCs [12]. Protective effects of calcium antagonists against calcium overload in hepatocytes have been investigated in vitro [13][14][15] and in vivo [16][17][18]. Brecht and de Groot [19] have reported that calcium channel blockers largely improved the energetic situation of the hepatocytes under hypoxic injury.…”