Protective Effects of Tualang Honey against Oxidative Stress and Anxiety-Like Behaviour in Stressed Ovariectomized Rats

Abstract

The present study aims to evaluate the antioxidant and anxiolytic-like effect of Tualang honey in stressed ovariectomized (OVX) rats. The animals were divided into; (i) nonstressed sham-operated control rats, (ii) sham-operated control rats exposed to stress, (iii) nonstressed OVX rats, (iv) OVX rats exposed to stress, (v) OVX rats exposed to stress and treated with 17 β-oestradiol (E2) (20 μg daily, sc), and (vi) OVX rats exposed to stress and treated with Tualang honey (0.2 g/kg body weight, orally). The open field test was used to evaluate the anxiety-like behaviour and ELISA kits were used to measure oxidant/antioxidant status of the brain homogenates. The result showed that anxiety-like behavior was significantly increased in stressed OVX compared to other groups, and administering either E2 or Tualang honey significantly decreased anxiety-like behaviour in stressed OVX rats. The levels of malondialdehyde (MDA) and protein carbonyl (PCO) were significantly decreased while the levels/activities of superoxide dismutase (SOD), glutathione S-transferases (GST), glutathione peroxidase (GPx), and glutathione reductase (GR) were significantly increased in the brain homogenates of treated stressed OVX groups compared to untreated stressed OVX. In conclusion, Tualang honey has protective effects against brain oxidative stress and may be useful alternative anxiolytic agent especially for postmenopausal women.

Natural Products and Their Bioactive Compounds: Neuroprotective Potentials against Neurodegenerative Diseases

“…Hence, honey confers its neuroprotection against the deleterious effect of kainic acid through its antioxidants and anti-inflammatory and antiapoptotic properties, thereby protecting brain from neuronal loss and neurodegeneration. In addition, honey has been reported to reduce brain oxidative stress and improve morphological impairment of the hippocampus and medial prefrontal cortex in stressed rats [50][51][52][53][54][55]. Honey also attenuates the cognitive impairment caused by chronic cerebral hypoperfusion-induced neurodegeneration [56] and ameliorates oxidative stress in the rat midbrain against repeated paraquat exposure [57].…”

Influence of intravenous Egyptian fennel honey infusion on the antioxidant activities and some haemo-indices in healthy goats

“…Similar these positive results were obtained in healthy tested models as with Malaysian honeys (nenas H. in healthy university student, 14 gelam H. in young and aged rats 57 or with tualang H. in normal male mice 56 ). Moreover,the increased GPX and SOD with the decreased MDA was obtained with Malayian tualang honey in stressed oxidative smokers 58 or female athletes 61 and in animal induced stress oxidative ovariectomized 59 or diabetic rats. 60 Otherwise, Iranian multifloral honey 62 ameliorated the increased MDA content and reduced the activity of SOD in stressed oxidative rats due to the inhibition of pro -inflammatory cytokines: TNF-, IL-1 , and IL-6 by the action of the polyphenols, flavonoids, and caffeic acid phenethyl ester.…”

Tualang Honey Ameliorates Hypoxia-induced Memory Deficits by Reducing Neuronal Damage in the Hippocampus of Adult Male Sprague Dawley Rats

“…29 Correspondingly, another study reported that oxidative stress markers significantly reduced and increased antioxidative enzymes further supporting the protective effect of Tualang honey against brain oxidative stress. 30 However, to our knowledge, no study has investigated the role of Tualang honey on learning and memory of rats in hypoxic condition. Thus, the present study aims to evaluate the efficacy of Tualang honey to ameliorate hypoxiainduced memory deficit in adult male Sprague-Dawley rats.…”