Protective Effects of the Fermented Milk Kefir on X-Ray Irradiation-Induced Intestinal Damage in B6C3F1 Mice

Abstract

Gastrointestinal damage associated with radiation therapy is currently an inevitable outcome. The protective effect of Kefir was assessed for its usefulness against radiation-induced gastrointestinal damage. A Kefir supernatant was diluted by 2-or 10-fold and administered for 1 week prior to 8 Gray (Gy) X-ray irradiation at a dose rate of 2 Gy/min, with an additional 15 d of administration post-irradiation. The survival rate of control mice with normal drinking water dropped to 70% on days 4 through 9 post-irradiation. On the other hand, 100% of mice in the 10-and 2-fold-diluted Kefir groups survived up to day 9 post-irradiation (p<0.05 and p<0.01, respectively). Examinations for crypt regeneration against 8, 10 and 12 Gy irradiation at a dose rate of 4 Gy/min revealed that the crypt number was significantly increased in the mice administered both diluted Kefir solutions (p<0.01 for each). Histological and immunohistochemical examinations revealed that the diluted Kefir solutions protected the crypts from radiation, and promoted crypt regeneration. In addition, lyophilized Kefir powder was found to significantly recover the testis weights (p<0.05), but had no effects on the body and spleen weights, after 8 Gy irradiation. These findings suggest that Kefir could be a promising candidate as a radiation-protective agent.

Removal Efficiency of Radioactive Cesium and Iodine Ions by a Flow-Type Apparatus Designed for Electrochemically Reduced Water Production

“…Therefore, molecular hydrogen and Pt nanoparticles dissolved in ERW could synergistically contribute to protect gastrointestinal damage caused by ingested radioactive foodstuffs. Furthermore, to maximize protective efficacy against radiation-induced gastrointestinal damage, the consumption of various supplemental foods such as naringin [42], probiotics [57], [66], Kefir [79], melatonin [80] and curcumin [81] are reported to be beneficial.…”

Kefir, Kımız, Süt ve Yoğurt Uygulamasının Fare Dalağında Plazma Hücresi ve Mast Hücrelerinin Dağılımı Üzerine Etkileri

“…Teruya et al [22] found that kefir both increase the growth and live weight, in parallel to study of Carnevell et al [23] , in a study on the protective effects of kefir and fermented milk in case of X-ray irradiation-induced intestinal damage. Also in this study, similar to studies [23,24] reporting that beneficial microbial food increase live weight, it was detected that kefir, koumiss, milk and yoghurt administration increases live weight and that this increase is statistically significant (P<0.05).…”

“…Beyond the inherent high nutritional value as a source of protein and calcium, kefir is believed to be beneficial to health in the countries where kefir is an essential part of the diet culture for a long time [26,27] . Effects of kefir on antioxidant and growth factors have been shown in several studies [22,[28][29][30][31][32][33] found that the protective effect of kefir is more than vitamin E in oxidative damage induced by CCl 4 in mice, by reducing lipid peroxidation and increasing reduced glutathione and glutathione peroxidase levels. In recent years in the mentality of a healthy diet, nutrition support with antioxidant-rich foods to prevent damages causing oxidative stress in the body has risen to the forefront.…”

X/XO or H2O2 induced IPEC-J2 cell as a new in vitro model for studying apoptosis in post-weaning piglets

“…We previously proved that weaning stress is the primary cause of the promotion of free radical generation (Zhu et al 2012) and is associated with an increase in free radicals ; therefore, we established the apoptosis model using ROS induction. There are many ways to induce ROS in cell culture media, including X-ray irradiation (Teruya et al 2013), hypoxic/re-oxygenation (Wasa et al 2005) or certain drugs (Li et al 2012;Zhang et al 2011); however, the most common and conventional method to model weaning in piglets should be hydrogen peroxide and/or xanthine/xanthine oxidase (X/XO) induction of apoptosis .…”