Protective Effects of the Alga Fucoidan Against Amyloid-β-Induced Neurotoxicity in SH-SY5Y Cells

Nagata, Miki; Tsuji, Mayumi; Oguchi, Tamio; Momma, Yutaro; Nohara, Tetsuhito; Ohashi, Hiroyuki; Ito, Naohito; Yamamoto, Ken; Udaka, Yuko; Sasaki, Akira; Kiuchi, Yuji; Numazawa, Satoshi

doi:10.1248/bpbreports.4.6_206

Cited by 3 publications

(1 citation statement)

References 41 publications

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“…To detect the peroxidation of phospholipids in the membranes of cells, SH-SY5Y cells (1.0 × 10 6 cells/mL) were stained with 5 µM diphenyl-1-pyrenylphosphine (DPPP; Thermo Fisher Scientific K.K, Waltham, MA, USA) in DMSO at 37 °C for 10 min, and then phospholipid peroxidation was measured according to a previously described method [ 61 ]. Briefly, the stained cells were exposed to Aβ 1–42 or treated with Aβ 1–42 + Cur, Aβ 1–42 + FA, or Aβ 1–42 + Cur + FA for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Combined Treatment with Curcumin and Ferulic Acid Suppressed the Aβ-Induced Neurotoxicity More than Curcumin and Ferulic Acid Alone

Ohashi

Tsuji

Oguchi

et al. 2022

IJMS

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Alzheimer’s disease (AD) is a neurodegenerative disease that leads to progressive cognitive decline. Several effective natural components have been identified for the treatment of AD. However, it is difficult to obtain conclusive evidence on the safety and effectiveness of natural components, because a variety of factors are associated with the progression of AD pathology. We hypothesized that a therapeutic effect could be achieved by combining multiple ingredients with different efficacies. The purpose of this study was thus to evaluate a combination treatment of curcumin (Cur) and ferulic acid (FA) for amyloid-β (Aβ)-induced neuronal cytotoxicity. The effect of Cur or FA on Aβ aggregation using thioflavin T assay was confirmed to be inhibited in a concentration-dependent manner by Cur single or Cur + FA combination treatment. The effects of Cur + FA on the cytotoxicity of human neuroblastoma (SH-SY5Y) cells induced by Aβ exposure were an increase in cell viability, a decrease in ROS and mitochondrial ROS, and repair of membrane damage. Combination treatment showed an overall higher protective effect than treatment with Cur or FA alone. These results suggest that the combined action mechanisms of Cur and FA may be effective in preventing and suppressing the progression of AD.

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Section: Methodsmentioning

confidence: 99%