Protective effects of the Aeromonas phages pAh1-C and pAh6-C against mass mortality of the cyprinid loach (Misgurnus anguillicaudatus) caused by Aeromonas hydrophila

Jun, Jin Woo; Kim, Ji Hyung; Shin, Sook; Han, Jee Eun; Chai, Ji Young; Park, Taesung

doi:10.1016/j.aquaculture.2013.09.045

Cited by 78 publications

(96 citation statements)

References 27 publications

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“…Known by some as the oriental weather loach, the pond loach, the Japanese loach, and the cyprinid loach, M. anguillicaudatus is a major aquaculture species in Japan, Korea and other countries, where it typically inhabits rice paddies, ditches and streams. [27][28][29] Wu et al grew A. hydrophila to log phase and then infected them with phage AH1 at an MOI of 2. 26 The infected bacteria were then centrifuged and the pellet resuspended in Ringer's solution before being injected into the loach at 3 h intervals.…”

Section: Aeromonas Hydrophilamentioning

confidence: 99%

“…29 Healthy loaches with a mean weight of 15 g were injected intraperitoneally (i.p.) with either 2.6 £ 10 6 (trial 1) or 2.6 £ 10 7 (trial 2) CFU of A. hydrophila/ fish followed immediately by injection with either 3.0 £ 10 7 PFU of phage pAh1-C/fish or 1.7 £ 10 7 PFU of pAh6-C/fish.…”

Section: Aeromonas Hydrophilamentioning

confidence: 99%

“…29 The A. hydrophila was still injected at the same levels. Mortalities in controls without phages were 38% (trial 1) and 96% (trial 2) after 7 d. With phage treatment, mortalities in trial 1 were 17% and 12% with pAh1-C and pAh6-C treatments, respectively.…”

Section: Aeromonas Hydrophilamentioning

confidence: 99%

“…Results showed that the phage treatments did not have any negative effects on the fish. 29 Unfortunately, they did not report on any studies using a combination of the 2 phages, but they did acknowledge the possibility that combining the phages might enhance the efficiency of phage therapy.…”

Section: Aeromonas Hydrophilamentioning

confidence: 99%

See 3 more Smart Citations

Bacteriophage remediation of bacterial pathogens in aquaculture: a review of the technology

Richards

2014

Bacteriophage

110

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Section: Aeromonas Hydrophilamentioning

confidence: 99%

Section: Aeromonas Hydrophilamentioning

confidence: 99%

Section: Aeromonas Hydrophilamentioning

confidence: 99%

Section: Aeromonas Hydrophilamentioning

confidence: 99%

See 2 more Smart Citations

Bacteriophage remediation of bacterial pathogens in aquaculture: a review of the technology

Richards

2014

Bacteriophage

110

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“…to commercially used antibiotics has been of great concern [5,[7][8][9]. In our previous study, a virulent phage, pAh6-C, was reported, and its therapeutic potential was studied in a fish model of experimental infection using a multiple-antibiotic-resistant A. hydrophila strain, JUNAH [10]. In our previous study, a virulent phage, pAh6-C, was reported, and its therapeutic potential was studied in a fish model of experimental infection using a multiple-antibiotic-resistant A. hydrophila strain, JUNAH [10].…”

mentioning

confidence: 99%

Genomic structure of the Aeromonas bacteriophage pAh6-C and its comparative genomic analysis

et al. 2014

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pAh6-C is a virulent bacteriophage (phage), isolated from a river in Korea, that infects a multiple-antibiotic-resistant A. hydrophila strain, JUNAH. The double-stranded DNA genome of pAh6-C is composed of 53,744 bp with a G + C content of 52.83 %. The genome encodes 86 putative ORFs, four putative promoters, and seven transcriptional terminator regions. Genome sequence analysis of pAh6-C and comparative analysis with the homologous Shewanella phage Spp001 revealed that there is a high degree of similarity between pAh6-C and Spp001 in 50 of the 86 ORFs of pAh6-C. The results of this investigation indicate that pAh6-C is closely related to Spp001, especially in the genes coding for proteins involved in DNA metabolism.

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Genome characterization of a novel vibriophage VpKK5 (Siphoviridae) specific to fish pathogenic strain ofVibrio parahaemolyticus

Lal

Sano

Ransangan

2016

J. Basic Microbiol.

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Vibrio parahaemolyticus has long been known pathogenic to shrimp but only recently it is also reported pathogenic to tropical cultured marine finfish. Traditionally, bacterial diseases in aquaculture are often treated using synthetic antibiotics but concern due to side effects of these chemicals is elevating hence, new control strategies which are both environmental and consumer friendly, are urgently needed. One promising control strategy is the bacteriophage therapy. In this study, we report the isolation and characterization of a novel vibriophage (VpKK5), belonging to the family Siphoviridae that was specific and capable of complete lysing the fish pathogenic strain of V. parahaemolyticus. The VpKK5 exhibited short eclipse and latent periods of 24 and 36 min, respectively, but with a large burst size of 180 pfu/cell. The genome analysis revealed that the VpKK5 is a novel bacteriophage with the estimated genome size of 56,637 bp and has 53.1% G + C content. The vibriophage has about 80 predicted open reading frames consisted of 37 complete coding sequences which did not match to any protein databases. The analysis also found no lysogeny and virulence genes in the genome of VpKK5. With such genome features, we suspected the vibriophage is novel and could be explored for phage therapy against fish pathogenic strains of V. parahaemolyticus in the near future.

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Protective effects of the Aeromonas phages pAh1-C and pAh6-C against mass mortality of the cyprinid loach (Misgurnus anguillicaudatus) caused by Aeromonas hydrophila

Cited by 78 publications

References 27 publications

Bacteriophage remediation of bacterial pathogens in aquaculture: a review of the technology

Bacteriophage remediation of bacterial pathogens in aquaculture: a review of the technology

Genomic structure of the Aeromonas bacteriophage pAh6-C and its comparative genomic analysis

Genome characterization of a novel vibriophage VpKK5 (Siphoviridae) specific to fish pathogenic strain ofVibrio parahaemolyticus

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