Protective Effects of Shrimp Peptide on Dextran Sulfate Sodium-Induced Colitis in Mice

Abstract

Inflammatory bowel disease, an intestinal relapsing inflammatory disease, not only impairs gastrointestinal function but also increases the chances of developing colon cancer. Currently, the effects of shrimp peptide (SP) in mice model of ulcerative colitis (UC) are still unclear. In particular, it is uncertain whether SP affects the gut flora with UC mice. In this study, we investigated the anti-inflammatory effects of SP on a dextran sulfate sodium (DSS)-induced mouse model of UC. Firstly, the molecular weight of SP was mainly distributed in the range of 180–1,000 Da (61.95% proportion), and the amino acid composition showed that SP contained 17 amino acids, of which, the essential amino acids accounted for 54.50%. In vivo, oral SP significantly attenuated the severity of colitis, such as diarrhea, weight loss, and rectal bleeding. Furthermore, treatment with SP remarkably ameliorated intestinal barrier integrity, thus lowering the levels of the inflammatory cytokines and ameliorating antioxidant indices and intestinal injury indicators in the serum and colon. Lastly, the cecal contents were used to sequence and analyze the 16S rRNA genes of bacteria. Results suggested that treatment with SP could restore the balance of intestinal flora in modeled mice by regulating the abundance of pathogenic and beneficial bacteria. Furthermore, SP could significantly improve intestinal flora dysfunction in mice with UC. In summary, our findings show that SP has a prophylactic and therapeutic effect in UC in vivo, thereby highlighting its broad medicinal applications.

Preventive effect of tilapia skin collagen hydrolysates on ulcerative colitis mice based on metabonomic and 16 S rRNA gene sequencing

“…58 IL-6 was significantly increased in mice with DSS-induced colitis, which might be involved in the regulation of signaling pathways such as apoptosis, migration, and protein synthesis. 59 In the present study, TSCHs could decrease the content wileyonlinelibrary.com/jsfa of IL-6; however, the inhibitory effect of medium dose TSCHs (500 mg kg −1 ) on IL-6 was lower than that of low dose TSCHs (250 mg mg −1 ), which may mean that TSCHs do not act in a dose-dependent manner in DSS-induced UC. Moreover, the standard error of the medium dose of TSCHs (500 mg kg −1 ) was relatively large, which may be caused by individual differences in animals.…”

“…NF‐κB is considered to play a key role in the release of proinflammatory mediators; it has been reported that colonic inflammation can be alleviated by inhibiting the translocation of p65 from the cytosol to the nucleus, the phosphorylation and degradation of IκB‐α, and suppression of the expressions of NF‐κB‐related proteins 58 . IL‐6 was significantly increased in mice with DSS‐induced colitis, which might be involved in the regulation of signaling pathways such as apoptosis, migration, and protein synthesis 59 . In the present study, TSCHs could decrease the content of IL‐6; however, the inhibitory effect of medium dose TSCHs (500 mg kg −1 ) on IL‐6 was lower than that of low dose TSCHs (250 mg mg −1 ), which may mean that TSCHs do not act in a dose‐dependent manner in DSS‐induced UC.…”

The potential mechanism of Bletilla striata in the treatment of ulcerative colitis determined through network pharmacology, molecular docking, and in vivo experimental verification

“…The expression levels of inflammatory factors such as TNF-α, IL-1β, and IL-6 in UC were increased by abnormal activation of the PI3K/AKT signalling pathway (Li et al 2019a , b ; Li et al 2019a , b ). Furthermore, TNF-α is an important proinflammatory cytokine involved in the pathogenesis of UC, inducing the pathological opening of the intestinal tight junction barrier, increasing intestinal epithelial permeability, and disrupting intestinal barrier function (Xiang et al 2021 ; Shin et al 2020 ). Therefore, anti-TNF-α therapy has been widely used in clinical practice as a suitable method of UC treatment.…”

Dietary Oyster (Crassostrea gigas) Extract Ameliorates Dextran Sulfate Sodium-Induced Chronic Experimental Colitis by Improving the Composition of Gut Microbiota in Mice

“…Furthermore, OE intake could slightly restore the structure of the fecal microbiota to that observed in normal mice (control group) ( Figure 6 C). Other food components, including radish sprout [ 44 ] and shrimp peptide [ 45 ], have also been reported to improve gut bacterial diversity reduced by DSS administration. Thus, OE intake may improve α- and β-diversity, exacerbated by DSS administration.…”