Protective Effects of Shen-Yuan-Dan, a Traditional Chinese Medicine, against Myocardial Ischemia/Reperfusion Injury<i>In Vivo</i>and<i>In Vitro</i>

Liu, Hongxu; Shang, Juju; Chu, Fuyong; Li, Aiyong; Wu, Bao Qiang; Xie, Xinran; Liu, Weihong; Yang, Hongzhi; Tong, Tong

doi:10.1155/2013/956397

Cited by 14 publications

(22 citation statements)

References 31 publications

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“…These data suggest that the PI3K/Akt/mTORC1 signaling pathway effectively function following SYDC and inhibit the accumulation of lipids in macrophages by enhancing autophagy. Interestingly, our previous study also demonstrated that SYDC prevents the development of atherosclerosis and inflammatory reaction by inhibiting the activation of PI3K/Akt/NF-κB signaling pathway (Zhou et al, 2017), while another previous study also demonstrated that SYDC protects ischemic myocardium from ischemia-reperfusion injury and inhibits cell apoptosis in cardiomyocytes, and the mechanism underlying the cardioprotective effects is associated with activation of the PI3K/Akt pathway (Liu et al, 2013). Therefore, we speculate that PI3K/Akt may be a key signaling pathway for the cardiovascular protective effects of SYDC treatment.…”

Section: Discussionmentioning

confidence: 94%

Shen-Yuan-Dan Capsule Attenuates Atherosclerosis and Foam Cell Formation by Enhancing Autophagy and Inhibiting the PI3K/Akt/mTORC1 Signaling Pathway

et al. 2019

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Background and Aim: The phosphoinositide 3-kinase (PI3K)/Akt/mammalian target of rapamycin complex 1 (mTORC1) signaling pathway plays a crucial role in autophagy and inflammation. Our previous studies demonstrated that Shen-Yuan-Dan Capsule (SYDC), a Chinese medicine used for treating angina pectoris, has anti-atherosclerotic and anti-inflammatory effects in mice. However, its effects on autophagy and the PI3K/Akt/mTORC1 signaling pathway remain unclear. This study aimed to explore the effects of SYDC on autophagy and PI3K/Akt/mTORC1 signaling in the apolipoprotein E knockout (ApoE −/− ) mouse model and in macrophage-derived foam cells to delineate the underlying mechanism. Methods: After 6 weeks of high-fat diet, ApoE –/– mice were randomly grouped into control, Lipitor, low-SYDC (SYDC-L), middle-SYDC (SYDC-M), and high-SYDC (SYDC-H) groups (n = 10). The mice were intragastrically administered the respective treatment for 6 weeks. Murine RAW264.7 cells were stimulated with oxidized low-density lipoprotein (ox-LDL) (80 µg/ml) for 24 h and then pretreated with SYDC freeze-dried powder for another 24 h. Cells treated with SYDC were co-cultured for 24 h with LY294002, tricirbine, and rapamycin to investigate the effects on the PI3K/Akt/mTORC1 signaling pathway. Results: SYDC ameliorated blood lipid levels, reduced the atherosclerotic index and plaque areas in the aortic root in mice, and inhibited total cholesterol (TC) levels and cholinesterase (ChE)/TC ratios in ox-LDL stimulated macrophages. Moreover, SYDC up-regulated Beclin1 and LC3II/I proteins in mice and in the ox-LDL–stimulated macrophages. Moreover, SYDC inhibited AKT phosphorylation at Ser473 and mTOR phosphorylation at Ser2448 in mice and in ox-LDL–stimulated macrophages. Furthermore, SYDC’s inhibitory of ChE/TC ratios in ox-LDL–stimulated macrophages was not changed by selective inhibition of the PI3K/Akt/mTORC1 pathway. Conclusions: Our results highlight that SYDC treatment attenuates foam cell formation by promoting autophagy via inhibiting activation of the PI3K/Akt/mTORC1 signaling pathway. This study provides new insights into the molecular mechanism underlying SYDC’s therapeutic potential for treating atherosclerosis.

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Section: Discussionmentioning

confidence: 94%

Shen-Yuan-Dan Capsule Attenuates Atherosclerosis and Foam Cell Formation by Enhancing Autophagy and Inhibiting the PI3K/Akt/mTORC1 Signaling Pathway

et al. 2019

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“…With the development of thrombolysis, percutaneous transluminal coronary angioplasty and coronary artery bypass grafting, myocardial IR injury has become the main obstacle in obtaining the optimal therapeutic effect from ischemic heart disease during reperfusion treatment ( 28 ). Following myocardial IR therapy, the heart is confronted with further injury through ventricular remodeling ( 29 , 30 ).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑20b‑5p promotes ventricular remodeling by targeting the TGF‑β/Smad signaling pathway in a rat model of ischemia‑reperfusion injury

et al. 2018

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Myocardial ischemic injury results from severe impairment of the coronary blood supply and may lead to metabolic and ultrastructural changes, thereby causing irreversible damage. MicroRNA (miR)-20b-5p has been demonstrated to be involved in malignancies of the breast, colorectum, stomach, blood and oropharynx. The present study aimed to investigate the effects of miR-20b-5p on ventricular remodeling following myocardial ischemia-reperfusion (IR) injury in rats by targeting small mothers against decapentaplegic homolog 7 (Smad7) via the transforming growth factor-β (TGF-β)/Smad signaling pathway. A total of 70 adult male Sprague-Dawley rats were divided into seven groups: Sham group, IR group, negative control group, miR-20b-5p mimics group, miR-20b-5p inhibitors group, small interfering RNA (siRNA)-Smad7 group, and miR-20b-5p inhibitors + siRNA-Smad7 group. Dual luciferase reporter gene assays were used to verify the association between miR-20b-5p and Smad7. Myocardial infarction size, myocardial collagen volume fraction and perivascular collagen area were detected separately using triphenyltetrazolium chloride and Masson's staining. The rate of positive expression of Smad7 was detected using immunohistochemistry, and the expression levels of miR-20b-5p, TGF-β1, Smad3 and Smad7 were detected using reverse transcription-quantitative polymerase chain reaction and western blot analyses. The findings revealed that miR-20b-5p inhibited Smad7. Compared with the sham group, the other six groups had increased myocardial infarction size, myocardial collagen, and expression of miR-20b-5p, TGF-β1 and Smad3, and decreased expression of Smad7. Compared with the IR group, the miR-20b-5p mimics group and the siRNA-Smad7 group had increased myocardial infarction size and myocardial collagen, increased expression of TGF-β1 and Smad3, and decreased expression of Smad7. The expression of miR-20b-5p was markedly increased in the miR-20b-5p mimics group, but did not differ significantly from that in the siRNA-Smad7 group. The results demonstrated that miR-20b-5p promoted ventricular remodeling following myocardial IR injury in rats by inhibiting the expression of Smad7 through activating the TGF-β/Smad signaling pathway.

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“…The Chinese cockroach, Eupolyphaga sinensis (Corydiidae), is a key ingredient in a number of traditional Chinese medicinal products including Shu-Mai Tang, Dahuang Zhechong pill, Shensong Yangxin capsule, Shen-Yuan-Dan, Yi Guan Jin and Yaotongning 131 , 132 , 133 , 134 , 135 , 136 . Shu-Mai Tang is prescribed for the treatment of ischemic heart disease and was found to have a therapeutic effect against inflammation-induced myocardial fibrosis leading to the attenuation of left ventricular remodeling and improved cardiac function 131 .…”

Section: Blattodea (Cockroaches and Termites)mentioning

confidence: 99%

“…The Chinese herb modified Yi Guan Jian is an effective regimen that is usually used in outpatients with chronic liver diseases such as fibrosis and cirrhosis 134 . Shen-Yuan-Dan is used for the treatment of ischemic heart disease, proven to relieve angina and reduces cardiac dysfunction 135 Traditional Chinese medicine Yaotongning Capsule is a common remedy to treat rheumatism in China and possesses diverse biological activities including anti-inflammation 136 .…”

Section: Blattodea (Cockroaches and Termites)mentioning

confidence: 99%

Insects: an underrepresented resource for the discovery of biologically active natural products

Seabrooks¹,

Hu²

2017

Acta Pharmaceutica Sinica B

109

123

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Nature has been the source of life-changing and -saving medications for centuries. Aspirin, penicillin and morphine are prime examples of Nature׳s gifts to medicine. These discoveries catalyzed the field of natural product drug discovery which has mostly focused on plants. However, insects have more than twice the number of species and entomotherapy has been in practice for as long as and often in conjunction with medicinal plants and is an important alternative to modern medicine in many parts of the world. Herein, an overview of current traditional medicinal applications of insects and characterization of isolated biologically active molecules starting from approximately 2010 is presented. Insect natural products reviewed were isolated from ants, bees, wasps, beetles, cockroaches, termites, flies, true bugs, moths and more. Biological activities of these natural products from insects include antimicrobial, antifungal, antiviral, anticancer, antioxidant, anti-inflammatory and immunomodulatory effects.

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Protective Effects of Shen-Yuan-Dan, a Traditional Chinese Medicine, against Myocardial Ischemia/Reperfusion InjuryIn VivoandIn Vitro

Cited by 14 publications

References 31 publications

Shen-Yuan-Dan Capsule Attenuates Atherosclerosis and Foam Cell Formation by Enhancing Autophagy and Inhibiting the PI3K/Akt/mTORC1 Signaling Pathway

Shen-Yuan-Dan Capsule Attenuates Atherosclerosis and Foam Cell Formation by Enhancing Autophagy and Inhibiting the PI3K/Akt/mTORC1 Signaling Pathway

MicroRNA‑20b‑5p promotes ventricular remodeling by targeting the TGF‑β/Smad signaling pathway in a rat model of ischemia‑reperfusion injury

Insects: an underrepresented resource for the discovery of biologically active natural products

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