Protective Effects of Selenium on Hepatotoxicity Caused by Subacute Experimental Combined Exposure to Cadmium and Lead in Rats

Şlencu, Bogdan Gabriel

doi:10.31925/farmacia.2018.5.18

Cited by 9 publications

(4 citation statements)

References 49 publications

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“…The compounds of interest were dissolved in the drinking water. The experimental groups, with the corresponding elements dosage were [19,31,51,67]: Control group (only distilled water); Se group (Se -0.2 mg/L); Cd group (Cd -150 mg/L); Pb group (Pb -300 mg/L); Cd + Pb group (Cd -150 mg/L and Pb -300 mg/L); Cd + Se group (Cd -150 mg/L and Se -0.2 mg/L); Pb + Se group (Pb -300 mg/L and Se -0.2 mg/L); Cd + Pb + Se group (Cd -150 mg/L, Pb -300 mg/L and Se -0.2 mg/L). The period of exposure was 56 days [28].…”

Section: Methodsmentioning

confidence: 99%

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Testicular Injuries Due to Subacute Combined Exposure to Cadmium and Lead in Rats

SOLCAN¹

2022

FARMACIA

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Cadmium and lead are toxic heavy metals, while selenium is an essential element that exerts antioxidant effects. The purpose of this study was to assess the effects of sodium selenite coexposure in rats with testicular injuries induced by cadmium and lead subacute exposure. A number of 48 male Wistar rats were used, randomly divided into 8 experimental groups (n = 6). The animals were exposed for 56 days to the elements through the drinking water. The groups were: Control group; Se group; Cd group; Pb group; Cd + Pb group; Cd + Se group; Pb + Se group; Cd + Pb + Se group. Testes from rats exposed to cadmium, lead or cadmium simultaneously with lead showed various types of histological damage, overexpression of p53, COX-2, Bcl-2, caspase-3 and TUNEL, as well as accumulation of cadmium or lead in particular areas from testes sections, as evidenced by environmental scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (ESEM-EDAX). Coexposure to selenium partially alleviated histological damage and expression of the proteins involved in apoptosis. It caused a decreased accumulation of cadmium and lead and selenium accumulation in particular areas from testes sections. Exposure of rats to the toxic elements at the mentioned levels exerted significant testicular toxicity while selenium exerted moderate protective effects. RezumatCadmiul și plumbul sunt metale grele toxice iar seleniul este un element esențial care exercită efecte antioxidante. Scopul acestui studiu a fost de a evalua efectele coexpunerii la selenit de sodiu a șobolanilor cu leziuni testiculare induse de expunerea subacută la cadmiu și plumb. Au fost utilizați 48 de șobolani masculi rasa Wistar, distribuiți în 8 loturi (n = 6). Animalele au fost expuse timp de 56 de zile la elemente prin intermediul apei de băut. Loturile au fost: controlul; lotul Se; lotul Cd; lotul Pb; lotul Cd + Pb; lotul Cd + Se; lotul Pb + Se; lotul Cd + Pb + Se. Testiculele provenind de la șobolanii expuși la cadmiu, plumb sau cadmiu simultan cu plumb au prezentat diferite tipuri de leziuni histologice, supraexpresia p53, COX-2, Bcl-2, caspaza-3 și TUNEL, precum și acumularea cadmiului sau plumbului în anumite zone din secțiunile testiculare, fapt evidențiat prin microscopie electronică de scanare cuplată cu spectroscopie de raze X cu dispersie de energie. Coexpunerea la seleniu a atenuat parțial leziunile histologice și expresia proteinelor implicate în apoptoză și a determinat o acumulare redusă de cadmiu și plumb și acumularea seleniului în zone specifice din secțiunile testiculare. Expunerea șobolanilor la elementele toxice în dozele menționate s-a manifestat prin toxicitate testiculară semnificativă iar seleniul a manifestat efecte protectoare moderate.

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Section: Methodsmentioning

confidence: 99%

“…The approximate daily intakes of selenium, cadmium and lead per kg bw were calculated based on the approximate water consumption and the concentrations of the elements in the drinking water. The means for the entire experimental period are given in Table I [67].…”

Section: Methodsmentioning

confidence: 99%

Testicular Injuries Due to Subacute Combined Exposure to Cadmium and Lead in Rats

SOLCAN¹

2022

FARMACIA

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“…Hepatocyte involvement causes the release of enzymes into the extracellular compartment, then some liver enzymes have been tested, namely alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT) and alkaline phosphatase (ALP). The increase in transaminases reflects cell damage, particularly in the liver [22,36]. ALAT is cytosolic localization, while ASAT is mainly mitochondrial [3].…”

Section: Markers Of Hepatic Functionmentioning

confidence: 99%

Oral Acute Toxicity and Red Blood Cytotoxicity of the Medicinal Halophyte Limoniastrum Guyonianum Leaf Extract

Benkhaled¹

2020

FARMACIA

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Limoniastrum guyonianum is widely used traditionally in North Africa as decoction or infusion to treat various types of infections. However, safety assessment of this medicinal halophyte is still lacking. The aim of this study was to investigate the safety of the aqueous leaves extract by determining the oral acute toxicity effect on adult mice and cytotoxicity on human red blood cells. No mortality or specific toxic signs were observed. The LD50 can then be estimated to be greater than 5000 mg/kg. The recommended dose used in humans will be a maximum of 500 mg/kg. Histopathological examinations showed alterations that affected both renal and hepatic tissues, being the kidneys more sensitive. Indeed, glomerular necrosis appeared with the first ingested dose (1000 mg/kg bw). In contrast, focal hepatocyte necrosis was noticeable only at a concentration of 5000 mg/kg bw. The cytotoxic study showed a slight haemolysis of 13.52% which demonstrate that the extract does not express a haemolytic activity. Hence, the extract can be safety utilized in folkloric popular medicine as an oral anti-infective. Rezumat Limoniastrum guyonianum este utilizată tradițional pe scară largă în Africa de Nord, sub formă de decoct sau infuzie pentru tratarea diferitelor tipuri de infecții. Cu toate acestea, evaluarea siguranței acestei plante halofite nu a fost încă studiată. Scopul acestui studiu a fost de a investiga siguranța utilizării extractului apos de frunze prin determinarea toxicitații acute la șoarecii adulți și a citotoxicității asupra globulelor roșii umane. Nu au fost observate semne toxice specifice. DL50 estimată a fost mai mare de 5000 mg/kg. Doza recomandată la om se estimează la maximum 500 mg/kg. Examenele histopatologice au arătat modificări care au afectat atât țesuturile renale, cât și hepatice, rinichii fiind mai sensibili. Necroza glomerulară a apărut o dată cu prima doză ingerată (1000 mg/kg corp). În schimb, necroza hepatocitară focală a fost vizibilă doar la o concentrație de 5000 mg/kg corp. Studiul citotoxic a arătat o ușoară hemoliză, care demonstrează că extractul nu exprimă o activitate hemolitică. Prin urmare, extractul poate fi utilizat în siguranță ca antiinfecțios oral.

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“…Cd inhibits Se detoxi cation and forms volatile methylated Se compounds, so results in increasing Se retained in liver [15][16][17]. However, Cd in rats which causes testis injury can be protected by Se [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Comparative Study of the Biochemical Behavior of some Highly Toxic Minerals on Selenosis in Rats

Aljerf¹,

Williams²,

Ajong³

et al. 2020

Preprint

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Objective: An investigation of the potential influences of As, Hg, Tl and Pb on Se metabolism, which can suggest new drugs to cope the poisonousness of Se. Methods: Chronic cases were carried out with the nursing of rats with a purified diet of water-soluble vitamin mix, fat-soluble vitamin mix, saccharides, oil, and salts. One week after the basic diet, the rats taken Se have received diets containing 10 ppm of the element as sodium selenite or selenate added in the salts. Result: As, Hg, and Tl were repressed the volatized Se excreted from the lungs. As has assisted the biliary excretion of Se and inhibited the chronic selenosis. Tl has increased the retention of Se in the liver and kidney, but, had no chronic effect on the amount of Se deposited in all the studied tissues. Similarly, Hg has increased the retention of Se but in the spleen and carcass of rats indicating to the high Se concentration in blood. Hg and Tl have inhibited the Se in urine. Conclusion: No effects of the doses rich in As, Hg, and Tl on the Se excretion in fecal. Even though, we suggest As as a possible medication to chronic selenosis.

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Protective Effects of Selenium on Hepatotoxicity Caused by Subacute Experimental Combined Exposure to Cadmium and Lead in Rats

Cited by 9 publications

References 49 publications

Testicular Injuries Due to Subacute Combined Exposure to Cadmium and Lead in Rats

Testicular Injuries Due to Subacute Combined Exposure to Cadmium and Lead in Rats

Oral Acute Toxicity and Red Blood Cytotoxicity of the Medicinal Halophyte Limoniastrum Guyonianum Leaf Extract

Comparative Study of the Biochemical Behavior of some Highly Toxic Minerals on Selenosis in Rats

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