Protective effects of Schisandrin on high glucose-induced changes of RhoA and eNOS activity in human umbilical vein endothelial cells

Zhang, Rong-Jin; Hao, Huiyao; Liu, Qingjuan; Zuo, Hong-Ye; Chang, Ying-Na; Zhong-ji, Zhi; Guo, Peng-peng; Hao, Yongmei

doi:10.1016/j.prp.2018.02.010

Cited by 4 publications

(4 citation statements)

References 30 publications

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“…EA and MOVAS cells were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum, 1% MEM non-essential amino acids, penicillin, and streptomycin. For culture under high-glucose conditions, cells were exposed to medium containing 30 mM glucose for 48 hours [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

Ginsenoside Rg-1 prevents elevated cytosolic Ca2+ via store-operated Ca2+ entry in high-glucose–stimulated vascular endothelial and smooth muscle cells

Han

Shin

et al. 2022

BMC Complement Med Ther

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Background Ginsenoside Rg-1 (Rg-1), a triterpenoid saponin abundantly present in Panax ginseng, is a type of naturally occurring steroid with known anti-diabetic and anti-inflammatory effects. In this study, we sought to confirm the effects and mechanisms of action of Rg-1 on store-operated Ca2+ entry (SOCE) in human vascular endothelial cell line (EA) and murine aortic vascular smooth muscle cell line (MOVAS) cells exposed to high glucose. Methods Cytosolic Ca2+ concentrations in EA and MOVAS cells were measured by monitoring fluorescence of the ratiometric Ca2+-indicator, Fura-2 AM. Results High glucose significantly increased Ca2+ influx by abnormally activating SOCE in EA and MOVAS cells. Notably, this high glucose-induced increase in SOCE was restored to normal levels in EA and MOVAS cells by Rg-1. Moreover, Rg-1 induced reductions in SOCE in cells exposed to high glucose were significantly inhibited by the plasma membrane Ca2+ ATPase (PMCA) blocker lanthanum, the Na+/K+-ATPase blocker ouabain, or the Na+/Ca2+ exchanger (NCX) blockers Ni2+ and KB-R7943. These observations suggest that the mechanism of action of Rg-1 inhibition of SOCE involves PMCA and Na+/K+-ATPase, and an increase in Ca2+ efflux via NCXs in both EA and MOVAS cells exposed to high glucose. Conclusions These findings indicate that Rg-1 may protect vascular endothelial and smooth muscle cells from Ca2+ increases following exposure to hyperglycemic conditions.

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Section: Methodsmentioning

confidence: 99%

Ginsenoside Rg-1 prevents elevated cytosolic Ca2+ via store-operated Ca2+ entry in high-glucose–stimulated vascular endothelial and smooth muscle cells

Han

Shin

et al. 2022

BMC Complement Med Ther

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“…However, further analysis on diabetic rats, subjected to overnight fasting, suggested that a SCE preparation improved glucose homeostasis by the promotion of insulin sensitivity, but not secretion capacity [81]. In accordance with that conclusion, recent studies demonstrated SCE protective activity against the production of advanced glycation end-products (AGEs), which are harmful to endothelial cells [82]. AGEs are produced through non-enzymatic reactions during hyperglycaemia and promote ROS production, which attenuates the expression and activity of endothelial nitric oxide synthase (eNOS), leading to a reduced NO level, endothelium dysfunction, and finally to the formation of atheromatous plaques.…”

Section: Health Promoting Effects Of Sce Constituentsmentioning

confidence: 94%

“…AGEs are produced through non-enzymatic reactions during hyperglycaemia and promote ROS production, which attenuates the expression and activity of endothelial nitric oxide synthase (eNOS), leading to a reduced NO level, endothelium dysfunction, and finally to the formation of atheromatous plaques. It was shown that human umbilical vein endothelial (HUVEC) cell incubation with SCE elevated the expression and activity of eNOS, via downregulation of RhoA/Rho kinase activity; therefore, SCE was able to reduce hyperglycaemia-induced microvascular complications [82].…”

Section: Health Promoting Effects Of Sce Constituentsmentioning

confidence: 99%

Potential of Schisandra chinensis (Turcz.) Baill. in Human Health and Nutrition: A Review of Current Knowledge and Therapeutic Perspectives

et al. 2019

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Schisandra chinensis (Turcz.) Baill. (SCE) is a plant with high potential for beneficial health effects, confirmed by molecular studies. Its constituents exert anti-cancer effects through the induction of cell cycle arrest and apoptosis, as well as inhibition of invasion and metastasis in cancer cell lines and experimental animals. SCE displays antimicrobial effects against several pathogenic strains. It has anti-diabetic potential, supported by hypoglycemic activity. A diet rich in SCE improves pancreatic functions, stimulates insulin secretion, and reduces complications in diabetic animals. SCE prevents lipid accumulation and differentiation of preadipocytes, indicating its anti-obesity potential. SCE exerts a protective effect against skin photoaging, osteoarthritis, sarcopenia, senescence, and mitochondrial dysfunction, and improves physical endurance and cognitive/behavioural functions, which can be linked with its general anti-aging potency. In food technology, SCE is applied as a preservative, and as an additive to increase the flavour, taste, and nutritional value of food. In summary, SCE displays a variety of beneficial health effects, with no side effects. Further research is needed to determine the molecular mechanisms of SCE action. First, the constituents responsible for its beneficial effects should be isolated and identified, and recommended as preventative nutritional additives, or considered as therapeutics.

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“…Sch has been shown to have many pharmacological activities (Qi et al, 2019). Studies showed that Sch relieves the damage caused by high glucose stimulation on human umbilical vein endothelial cells by lowering the activity of RhoA/Rho kinase and increasing the production of NO (Zhang et al, 2018). Sch also had protective effects against the occurrence of 6‐OHDA‐induced neurodegenerative diseases by enhancing cellular anti‐inflammatory and anti‐apoptotic effects (Yan et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Schisandrin improves lipopolysaccharide‐induced acute lung injury by inhibiting the inflammatory response in vivo and in vitro

Liu

Huang

et al. 2022

Journal of Food Biochemistry

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Acute lung injury (ALI) is characterized by an excessive inflammatory response, closely related to sepsis occurrence and development. It has been reported that Schisandrin (Sch) exhibits anti-inflammatory activity. However, whether the beneficial effects of Sch exists during ALI remains to be studied. In this study, the impact of Sch was evaluated by studying lung tissue damage, measuring the concentrations of pro-inflammatory factors, and the expression of apoptotic proteins in the LPS-induced ALI mice model. Protein expression of inflammation-related signaling pathway within the lung tissue and A549 cells were also measured. In addition, the effect of Sch on A549 cell apoptosis and inflammatory markers was also detected. Animal experiments demonstrated that pre-feeding Sch alleviated the production of inflammation mediators, abnormal pathological injuries, and blocked the progression of apoptotic events in the lung tissue. The in vitro experiments showed that Sch pretreatment reduced LPS upregulated interleukin-1β (IL-1β), IL-18, and IL-6 levels, and improved LPS-induced abnormal apoptosis. Sch and the pathway inhibitor AG490 also inhibited the expression levels of p-JAK2 and p-STAT3 in A549 cells. Moreover, pretreatment with Sch significantly inhibited the activation of NLRP3 inflammasomes, reduced inducible nitric oxide synthase, and cyclooxygenase 2 proteins expression during ALI in vitro and in vivo. Overall, Sch effectively alleviated ALI and provided a new mechanism to support the protective effect of Sch for sepsis-induced ALI. Practical implicationsALI is characterized by inflammatory injury of the lungs, which is an important cause of high morbidity and mortality in severe patients. Sch is considered as a botanical active ingredient with various pharmacological activities, such as neuroprotective and vascular protective effects. However, the effect of Sch on ALI and its mechanism remains largely unknown. Research data indicate that Sch exerts an anti-inflammatory effect by reducing the production of inflammatory factors and abnormal apoptosis of cells, further alleviating lung damage. The protective effect of Sch was associated with inhibition of the activation of NLRP3 and the JAK2/STAT3 inflammatory pathways. The study, therefore, confirmed that Sch has a potential as an effective drug to prevent ALI diseases.

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Protective effects of Schisandrin on high glucose-induced changes of RhoA and eNOS activity in human umbilical vein endothelial cells

Cited by 4 publications

References 30 publications

Ginsenoside Rg-1 prevents elevated cytosolic Ca2+ via store-operated Ca2+ entry in high-glucose–stimulated vascular endothelial and smooth muscle cells

Ginsenoside Rg-1 prevents elevated cytosolic Ca2+ via store-operated Ca2+ entry in high-glucose–stimulated vascular endothelial and smooth muscle cells

Potential of Schisandra chinensis (Turcz.) Baill. in Human Health and Nutrition: A Review of Current Knowledge and Therapeutic Perspectives

Schisandrin improves lipopolysaccharide‐induced acute lung injury by inhibiting the inflammatory response in vivo and in vitro

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