Protective effects of Rosmarinus tomentosus ethanol extract on thioacetamide-induced liver cirrhosis in rats

Galisteo, Milagros; Suárez, Antonio; Montilla, M. P.; Fernández, M. Isabel; Gil, Ángel; Navarro, M.

doi:10.1016/j.phymed.2004.06.024

Cited by 23 publications

(22 citation statements)

References 23 publications

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“…The increased liver weight/body weight ratio in TAA-treated animal was due to the accumulation of fat and degeneration in the liver. This result was in agreement with a previous report on the increased liver weight/body weight ratio [42]. Our result showed that ERZO could significantly accelerate the recovery of the liver damage.…”

Section: Discussionsupporting

confidence: 94%

In VivoEvaluation of Ethanolic Extract ofZingiber officinaleRhizomes for Its Protective Effect against Liver Cirrhosis

Bardi

Halabi

Abdullah

et al. 2013

BioMed Research International

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Zingiber officinale is a traditional medicine against various disorders including liver diseases.The aim of this study was to assess the hepatoprotective activity of the ethanolic extract of rhizomes of Z. officinale (ERZO) against thioacetamide-induced hepatotoxicity in rats. Five groups of male Sprague Dawley have been used. In group 1 rats received intraperitoneal (i.p.) injection of normal saline while groups 2–5 received thioacetamide (TAA, 200 mg/kg; i.p.) for induction of liver cirrhosis, thrice weekly for eight weeks. Group 3 received 50 mg/kg of silymarin. The rats in groups 4 and 5 received 250 and 500 mg/kg of ERZO (dissolved in 10% Tween), respectively. Hepatic damage was assessed grossly and microscopically for all of the groups. Results confirmed the induction of liver cirrhosis in group 2 whilst administration of silymarin or ERZO significantly reduced the impact of thioacetamide toxicity. These groups decreased fibrosis of the liver tissues. Immunohistochemistry assessment against proliferating cell nuclear antigen did not show remarkable proliferation in the ERZO-treated rats when compared with group 2. Moreover, factions of the ERZO extract were tested on Hep-G2 cells and showed antiproliferative activity (IC50 38–60 μg/mL). This study showed hepatoprotective effect of ERZO.

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Section: Discussionsupporting

confidence: 94%

In VivoEvaluation of Ethanolic Extract ofZingiber officinaleRhizomes for Its Protective Effect against Liver Cirrhosis

Bardi

Halabi

Abdullah

et al. 2013

BioMed Research International

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“…Such enzymatic activities were in line with the earlier reports [32]. The increase in serum enzymatic activities is related to hepatic parenchymal damage since ALT is released from mitochondrial and cytosolic localization from membranal sites, and cellular rupture allows the enzyme to escape into the blood [33].…”

Section: Discussionsupporting

confidence: 89%

“…Factoring the reduced body weight into the calculation yielded significantly high ratios of liver and spleen-to-body weight. The hepatocyte proliferation is a critical determinant for the survival of liver from an injury [32]. Based on this, the upregulation of the hepatocyte activity in response to the exposure to TAA toxicity is likely to be the cause of the recorded increase in the liver and spleen weights.…”

Section: Discussionmentioning

confidence: 99%

Protective Role ofPhyllanthus niruriExtract against Thioacetamide-Induced Liver Cirrhosis in Rat Model

Amin

Bilgen

Alshawsh

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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A preclinical study was performed to determine if the extract from Phyllanthus niruri (PN) plays a protective role against liver cirrhosis induced by thioacetamide (TAA) in rats. Initially, acute toxicity was tested and the results showed that the extract was benign when applied to healthy rats. Next, the therapeutic effect of the extract was investigated using five groups of rats: control, TAA, silymarin, and PN high dose and low dose groups. Significant differences were observed between the TAA group and the other groups regarding body and liver weights, liver biochemical parameters, total antioxidant capacity, lipid peroxidation, and oxidative stress enzyme levels. Gross visualization indicated coarse granules on the surface of the hepatotoxic rats' livers, in contrast to the smoother surface in the livers of the silymarin and PN-treated rats. Histopathological analysis revealed necrosis, lymphocytes infiltration in the centrilobular region, and fibrous connective tissue proliferation in the livers of the hepatotoxic rats. But, the livers of the treated rats had comparatively minimal inflammation and normal lobular architecture. Silymarin and PN treatments effectively restored these measurements closer to their normal levels. Progression of liver cirrhosis induced by TAA in rats can be intervened using the PN extract and these effects are comparable to those of silymarin.

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“…Further, portal hypertension and impaired bile acid metabolism have been reported in liver cirrhosis [48], both influencing the nutrient utilization. Biochemical analysis of serum markers of hepatobiliary diseases (liver cirrhosis) shows increased alkaline phosphatase and GGT [49,50], which has also been reported in this study ( Table 2). Extract treatment could cause a significant decrease in the elevated levels of these enzymes.…”

Section: Discussionsupporting

confidence: 84%

Dried peel fraction of Citrus sinensis partially reverses pathological changes in rat model of liver cirrhosis

Ali

Prasad

Naime

et al. 2010

Mediterr J Nutr Metab

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Citrus sinensis is a seasonal fruit. Its zester is rich in bioactive phytochemicals, such as limonene, b-sitosterol, and ascorbic acid, which possess pharmacological action. In this study, we report the effect of fraction prepared from dried peel of C. sinensis on biochemical and histopathological changes in rat model of liver cirrhosis. Liver cirrhosis was induced in rats by administering thioacetamide at a concentration of 0.03% in drinking water for 16 weeks. Thioacetamide was discontinued after 16 weeks and from the 18th week rats were given the extract orally for 9 weeks. Following the completion of the treatment, animals were killed and biochemical and histopathological changes associated with liver cirrhosis were evaluated. The treatment was found to reverse the elevated levels of alkaline phosphatase, c-glutamyl transferase, and other biochemical markers related to oxidative stress and selected drug metabolizing enzymes. Histopathology of the hepatic tissue confirmed the curative effect of the extract, and corroborated with the biochemical findings. HPTLC fingerprinting of the test fraction confirmed the presence of limonene, b-sitosterol, and ascorbic acid, which may partially explain the effect. The extract was also found to possess the anti-proliferative activity, determined by measuring the incorporation of radioactive thymidine by the hepatic DNA. The study indicates the inhibitory action of the test preparation on collagen accumulation in the extracellular matrix, and hence suggests its use as a potential therapeutic agent in liver fibrosis and cirrhosis.

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Protective effects of Rosmarinus tomentosus ethanol extract on thioacetamide-induced liver cirrhosis in rats

Cited by 23 publications

References 23 publications

In VivoEvaluation of Ethanolic Extract ofZingiber officinaleRhizomes for Its Protective Effect against Liver Cirrhosis

In VivoEvaluation of Ethanolic Extract ofZingiber officinaleRhizomes for Its Protective Effect against Liver Cirrhosis

Protective Role ofPhyllanthus niruriExtract against Thioacetamide-Induced Liver Cirrhosis in Rat Model

Dried peel fraction of Citrus sinensis partially reverses pathological changes in rat model of liver cirrhosis

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