Protective effects of Re-yan-ning mixture on Streptococcus pneumonia in rats based on network pharmacology

Abstract

Context: Re-yan-ning mixture (RYNM) is a new national drug approved by China's State Food and Drug Administration for the treatment of colds, simple pneumonia and acute bronchitis. Objective: To determine the mechanism of action of RYNM in the treatment of bacterial pneumonia. Materials and methods: Using the network pharmacology approach, the multiple components, component candidate targets and multiple therapeutic targets of RYNM were screened and functionally enriched. Also, we established a rat Streptococcus pneumonia model to verify the results of network pharmacology enrichment analysis. Forty male SPF Sprague Dawley rats were divided into four groups of 10 rats: control (normal saline), model (normal saline), levofloxacin-intervened and RYNM-intervened groups. IL-10, NOS2, COX-1, IL-6, TNF-a and NF-jB in serum and BALF were detected by ELISA. Western blot detected IL-17, IL-6, TNF-a, COX-2 and Bcl-2. Results: The network pharmacology approach successfully identified 48 bioactive components in RYNM, and 65 potential targets and 138 signal pathways involved in the treatment of Streptococcus pneumonia with RYNM. The in vivo experiments indicated that model group has visible inflammation and lesions while RYNM and levofloxacin groups have not. The RYNM exhibited its therapeutic effects on Streptococcus pneumonia mainly via the regulation of cell proliferation and survival through the IL-6/IL-10/IL-17, Bax/Bcl-2, COX-1/COX-2, NF-jB and TNF-a signalling pathways. Discussion and conclusions: The present study demonstrated the protective effects of RYNM on Streptococcus pneumonia, providing a potential mechanism for the treatment of bacterial pneumonia with RYNM.

A retrospective study of Reyanning mixture in elderly patients infected with SARS-CoV-2 Omicron variant

“…The results demonstrated the effectiveness of Reyanning mixture in the improvement of lung lesions, autoimmune function, enhancing gastro-intestinal function, as well as reducing the expression of inflammatory factors. Han et al (2021) used the network pharmacology approach, exhibited its anti-inflammation effects via regulating the cell proliferation, and the surviving pathways. Wang M. et al (2020) employed network pharmacology in addition to molecular docking methodologies and forecast the potential application of Reyanning mixture in treating COVID-19.…”