Protective Effects of Radon Inhalation on Carrageenan-Induced Inflammatory Paw Edema in Mice

Kataoka, Takahiro; Teraoka, Junichi; Sakoda, Akihiro; Nishiyama, Yuichi; Yamato, Keiko; Monden, Mayuko; Ishimori, Yuu; Nomura, Takaharu; Taguchi, Takehito; Yamaoka, Kunihiro

doi:10.1007/s10753-011-9364-y

Cited by 39 publications

(33 citation statements)

References 23 publications

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“…At 6 h after the last dose of CHU treatment, 50 lL of carrageenan dissolved in 0.9% saline (1% m/v) was injected into the right hind paw of rats. 19 Paw swelling volumes were measured at 30, 60, and 120 min after carrageenan administration. After the measurements of swelling volumes, the homogenates of right hind paw were used for immunoblotting.…”

Section: Animal Treatmentmentioning

confidence: 99%

Specific Oligopeptides in Fermented Soybean Extract Inhibit NF-κB-Dependent iNOS and Cytokine Induction by Toll-Like Receptor Ligands

Lee

et al. 2014

Journal of Medicinal Food

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The ethanol extract of fermented soybean from Glycine max (chungkookjang, CHU) has been claimed to have chemopreventive and cytoprotective effects. In the present study, we examined the inhibitory effect of CHU on inducible nitric oxide synthase (iNOS) and cytokine induction by toll-like receptor (TLR) ligands treatment and attempted to identify the responsible active components. Nitric oxide (NO) content and iNOS levels in the media or RAW264.7 cells were measured using the Griess reagent and real-time polymerase chain reaction assays. CHU treatment inhibited NO production and iNOS induction elicited by lipopolysaccharide (LPS, TLR4L) in a concentration-dependent manner. Tumor necrosis factor-α and interleukin-6 productions were also diminished. Peptidoglycans (TLR2/6L) and CpG-oligodeoxynucleotides (TLR9L) from CHU inhibited iNOS induction, but not poly I:C (TLR3L) or loxoribine (TLF7L). The anti-inflammatory effect resulted from the inhibition of nuclear factor-kappa B (NF-κB) through the inhibition of inhibitory-κB degradation. Of the representative components in CHU, specific oligopeptides (AFPG and GVAWWMY) had the ability to inhibit iNOS induction by LPS, whereas others failed to do so. Daidzein, an isoflavone used for comparative purposes, was active at a relatively higher concentration. In an animal model, oral administration of CHU to rats significantly diminished carrageenan-induced paw edema and iNOS induction. Our results demonstrate that CHU has anti-inflammatory effects against TLR ligands by inhibiting NF-κB activation, which may result from specific oligopeptide components in CHU. Since CHU is orally effective, dietary applications of CHU and/or the identified oligopeptides may be of use in the prevention of inflammatory diseases.

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Section: Animal Treatmentmentioning

confidence: 99%

Specific Oligopeptides in Fermented Soybean Extract Inhibit NF-κB-Dependent iNOS and Cytokine Induction by Toll-Like Receptor Ligands

Lee

et al. 2014

Journal of Medicinal Food

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“…In addition, a large number of patients are treated in various countries with traditional spa therapy (Japan [14–16], central Europe [17]). To assess the effects of radon, we have developed a radon-exposure system for small animals; using this system, we demonstrated that radon inhalation has anti-inflammatory effects and inhibits carrageenan-induced inflammatory paw edema [18]. We also demonstrated that radon inhalation inhibits the oxidative damage associated with CCl 4 -induced hepatopathy in mice, indicating that radon inhalation has anti-oxidative effects [19].…”

Section: Introductionmentioning

confidence: 99%

Comparative study on the inhibitory effects of antioxidant vitamins and radon on carbon tetrachloride-induced hepatopathy

Kataoka

Nishiyama

Yamato

et al. 2012

Journal of Radiation Research

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We have previously reported that radon inhalation activates anti-oxidative functions and inhibits carbon tetrachloride (CCl4)-induced hepatopathy. It has also been reported that antioxidant vitamins can inhibit CCl4-induced hepatopathy. In the current study, we examined the comparative efficacy of treatment with radon, ascorbic acid and α-tocopherol on CCl4-induced hepatopathy. Mice were subjected to intraperitoneal injection of CCl4 after inhaling approximately 1000 or 2000 Bq/m3 radon for 24 h, or immediately after intraperitoneal injection of ascorbic acid (100, 300, or 500 mg/kg bodyweight) or α-tocopherol (100, 300, or 500 mg/kg bodyweight). We estimated the inhibitory effects on CCl4-induced hepatopathy based on hepatic function-associated parameters, oxidative damage-associated parameters and histological changes. The results revealed that the therapeutic effects of radon inhalation were almost equivalent to treatment with ascorbic acid at a dose of 500 mg/kg or α-tocopherol at a dose of 300 mg/kg. The activities of superoxide dismutase, catalase, and glutathione peroxidase in the liver were significantly higher in mice exposed to radon than in mice treated with CCl4 alone. These findings suggest that radon inhalation has an anti-oxidative effect against CCl4-induced hepatopathy similar to the anti-oxidative effects of ascorbic acid or α-tocopherol due to the induction of anti-oxidative functions.

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“…For example, radon inhalation activates antioxidative functions in many organs of mice and inhibits CCl 4 -induced liver and renal damage [18, 32, 33], type-1 diabetes [8], and pain [8, 34]. Interestingly, the absorbed dose of radon is much lower than X-irradiation.…”

Section: Discussionmentioning

confidence: 99%

“…For example, in the search for new indications for radon therapy, we reported the responsiveness to radon of an antioxidant enzyme (a radical scavenger), superoxide dismutase (SOD), in mouse organs [7], and suggested that radon inhalation activated antioxidative functions in many organs of mice. In addition, it was demonstrated that radon inhalation has anti-inflammatory effects and inhibits carrageenan-induced inflammatory paw edema [8]. Furthermore, a clinical study was conducted using thoron, which is an isotope of radon.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Effects of Pretreatment with Radon on Acute Alcohol-Induced Hepatopathy in Mice

Toyota

Kataoka

Nishiyama

et al. 2012

Mediators of Inflammation

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We previously reported that radon inhalation activates antioxidative functions in the liver and inhibits carbon tetrachloride-induced hepatopathy in mice. In addition, it has been reported that reactive oxygen species contribute to alcohol-induced hepatopathy. In this study, we examined the inhibitory effects of radon inhalation on acute alcohol-induced hepatopathy in mice. C57BL/6J mice were subjected to intraperitoneal injection of 50% alcohol (5 g/kg bodyweight) after inhaling approximately 4000 Bq/m3 radon for 24 h. Alcohol administration significantly increased the activities of glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) in serum, and the levels of triglyceride and lipid peroxide in the liver, suggesting acute alcohol-induced hepatopathy. Radon inhalation activated antioxidative functions in the liver. Furthermore, pretreatment with radon inhibited the depression of hepatic functions and antioxidative functions. These findings suggested that radon inhalation activated antioxidative functions in the liver and inhibited acute alcohol-induced hepatopathy in mice.

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Protective Effects of Radon Inhalation on Carrageenan-Induced Inflammatory Paw Edema in Mice

Cited by 39 publications

References 23 publications

Specific Oligopeptides in Fermented Soybean Extract Inhibit NF-κB-Dependent iNOS and Cytokine Induction by Toll-Like Receptor Ligands

Specific Oligopeptides in Fermented Soybean Extract Inhibit NF-κB-Dependent iNOS and Cytokine Induction by Toll-Like Receptor Ligands

Comparative study on the inhibitory effects of antioxidant vitamins and radon on carbon tetrachloride-induced hepatopathy

Inhibitory Effects of Pretreatment with Radon on Acute Alcohol-Induced Hepatopathy in Mice

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