Protective effects of puerarin on acute lung and cerebrum injury induced by hypobaric hypoxia via the regulation of aquaporin (AQP) via NF-κB signaling pathway

Wang, Chi; Yan, Muyang; Jiang, Hui; Wang, Qi; Guan, Xu; Chen, Jingwen; Wang, Chengbin

doi:10.1016/j.intimp.2016.09.010

Cited by 31 publications

(25 citation statements)

References 47 publications

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“…Puerarin has a potential function in hepatic protection against liver injuries. In previous study, we have introduced that puerarin could protect against liver fibrosis via inhibiting PARP‐1 and further attenuation of NF‐κB activation, reactive oxygen species production, and mitochondrial dysfunction (Wang, Yan, et al, ). This time, we showed that PARP‐1 was also the target for puerarin in NAFLD.…”

Section: Discussionmentioning

confidence: 99%

“…Immunohistochemical detection of myeloperoxidase (MPO), an index of neutrophil accumulation, was performed as previously described (Montalto et al, ). Hepatic fibrosis was analyzed by Sirius Red according to earlier publication (Wang, Yan, et al, ). Immunostaining for α‐SMA was performed on paraffin sections with α‐SMA antibody (1:75, Abcam) according to standard methods in routine pathology.…”

Section: Methodsmentioning

confidence: 99%

“…Hepatic fibrosis was analyzed by Sirius Red according to earlier publication (Wang, Yan, et al, 2016). Immunostaining for α-SMA was performed on paraffin sections with α-SMA antibody (1:75, Abcam) according to standard methods in routine pathology.…”

Section: Histological Analyzesmentioning

confidence: 99%

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Puerarin protects against high‐fat high‐sucrose diet‐induced non‐alcoholic fatty liver disease by modulating PARP‐1/PI3K/AKT signaling pathway and facilitating mitochondrial homeostasis

Wang

Yang

Shang

et al. 2019

Phytotherapy Research

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As yet, there was no effective pharmacological therapy approved for non‐alcoholic fatty liver disease (NAFLD). Here, we aimed to evaluate the therapeutic potential of puerarin against NAFLD and explored the underlying mechanisms. C57BL/6J mice were fed with a high‐fat high‐sucrose (HFHS) diet with or without puerarin coadministration intragastrically. The levels of hepatocellular injury, steatosis, fibrosis, and mitochondrial and metabolism alteration were detected. First, puerarin ameliorated histopathologic abnormalities due to HFHS. We observed a marked increase in hepatic lipid content, inflammation, and fibrosis level, which were attenuated by puerarin. Possible mechanisms were related to puerarin‐mediated activation of PI3K/AKT pathway and further improvement in fatty acid metabolism. Puerarin restored the NAD+ content and beneficially affected the hepatic mitochondrial function, which attenuated HFHS‐induced steatosis and metabolic disturbances. Finally, hepatic PARP‐1 was activated due to excessive fat intake. Puerarin attenuated the PARP‐1 expression in HFHS‐fed mice, and PJ34, the PARP inhibitor, could mimic these protections of puerarin. However, pharmacological inhibition of PI3K disabled the protection of puerarin or PJ34 toward NAD+ refilling and mitochondrial homeostasis. In conclusion, our findings indicated that puerarin could be a promising and practical therapeutic strategy in NAFLD through modulating PARP‐1/PI3K/AKT signaling pathway and further facilitating mitochondrial function.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Puerarin protects against high‐fat high‐sucrose diet‐induced non‐alcoholic fatty liver disease by modulating PARP‐1/PI3K/AKT signaling pathway and facilitating mitochondrial homeostasis

Wang

Yang

Shang

et al. 2019

Phytotherapy Research

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“…The bronchoalveolar lavage fluid (BALF) are widely used for detecting biological molecules change in clinic and laboratory [16], and it have been reported that TNF-α and AQP4 in BALF are closely associated with the airway function, the elevated TNF-α expression in BALF may be related to inflammatory mechanism and causes ventilator-induced lung injury even high mortality [17], besides, increased inflammatory cytokines and AQP4 in BALF have been observed in the condition of hypobaric hypoxia [18], it’s noteworthy that even though TNF-α and AQP4 may related to the change of airway function, the role of TNF-α and AQP4 in the conditions of tracheostomy remain unclear, further studies on this issue are warranted.…”

Section: Introductionmentioning

confidence: 99%

The role of osmolality in saline fluid nebulization after tracheostomy: time for changing?

Wen

Cui

et al. 2016

BMC Pulm Med

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BackgroundSaline fluid nebulization is highly recommend to combat the complications following tracheostomy, yet the understandings on the role of osmolality in saline solution for nebulization remain unclear.ObjectivesTo investigate the biological changes in the early stage after tracheostomy, to verify the efficacy of saline fluid nebulization and explore the potential role of osmolality of saline nebulization after tracheostomy.MethodsSprague-Dawley rats undergone tracheostomy were taken for study model, the sputum viscosity was detected by rotational viscometer, the expressions of TNF-α, AQP4 in bronchoalveolar lavage fluid were assessed by western blot analysis, and the histological changes in endothelium were evaluated by HE staining and scanning electron microscopy (SEM).ResultsStudy results revealed that tracheostomy gave rise to the increase of sputum viscosity, TNF-α and AQP4 expression, mucosa and cilia damage, yet the saline fluid nebulization could significantly decrease the changes of those indicators, besides, the hypertonic, isotonic and hypertonic saline nebulization produced different efficacy.ConclusionsOsmolality plays an important role in the saline fluid nebulization after tracheostomy, and 3% saline fluid nebulization seems to be more beneficial, further studies on the role of osmolality in saline fluid nebulization are warranted.

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“…27 PR treatment exerted anti-inflammatory effects on the rat model of cerebral injury through the inhibition of NF-kB signaling pathway. 33 In addition, PR can modulate the PI3k/Akt signaling pathway to exert anti-inflammatory and antiapoptotic effects on cerebral and kidney injuries. 23,24 The PI3K/AKT signaling pathway not only regulates macrophage survival, migration, and proliferation but also orchestrates the response to different metabolic and inflammatory signals in macrophages.…”

mentioning

confidence: 99%

Therapeutic Effects of Puerarin Against Anterior Ischemic Optic Neuropathy Through Antiapoptotic and Anti-Inflammatory Actions

Wen²,

et al. 2019

Invest. Ophthalmol. Vis. Sci.

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Protective effects of puerarin on acute lung and cerebrum injury induced by hypobaric hypoxia via the regulation of aquaporin (AQP) via NF-κB signaling pathway

Cited by 31 publications

References 47 publications

Puerarin protects against high‐fat high‐sucrose diet‐induced non‐alcoholic fatty liver disease by modulating PARP‐1/PI3K/AKT signaling pathway and facilitating mitochondrial homeostasis

Puerarin protects against high‐fat high‐sucrose diet‐induced non‐alcoholic fatty liver disease by modulating PARP‐1/PI3K/AKT signaling pathway and facilitating mitochondrial homeostasis

The role of osmolality in saline fluid nebulization after tracheostomy: time for changing?

Therapeutic Effects of Puerarin Against Anterior Ischemic Optic Neuropathy Through Antiapoptotic and Anti-Inflammatory Actions

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