Protective Effects of Pomegranate Peel Extract on Paclitaxel Induced Primary Neuron Damage in Rats

Abstract

Aim: The combination of chemotherapeutics and pomegranate peel extract has been shown to have a stronger anti-cancer effect. However, there is no information about how pomegranate peel extract will affect the neurotoxicity that may develop due to cancer chemotherapy. In this study, we aimed to the effects of strong antioxidant pomegranate peel extract on paclitaxel induced primary neuron damage in newborn rats by biochemical and molecular analysis. Material and Method: Paclitaxel toxicity was induced in the primary neuron cell culture derived from newborn rats. PPE 200, 300 and 400 mg/ml doses were applied 2 hours before the paclitaxel for protective effect. Results: In the MTT analysis, paclitaxel reduced cell viability by caused neuronal damage at the end of 24 hours. PPE has demonstrated significant protective effect on cell viability by preventing paclitaxel toxicity at all doses. PPE was demonstrated its effects by reducing oxidative stress, increasing antioxidant capacity, and also by suppressing the TNF-α expression which is pro-inflammatory cytokine, and caspase 9 and 3 expression which are apoptotic proteins. Conclusion: PPE prevents primary neuron damage caused paclitaxel by its antioxidant, anti-inflammatory and anti-apoptotic effect.

The role of pomegranate seed oil on kidney and lung tissues in the treatment of sepsis: animal pre-clinical research

“…In this syndrome, as a result of atelectasis and intravascular thrombosis, ventilation impairment occurs. Inflammatory cells and factors induce oxidative stress and apoptosis [13]. MODS renal injury is a renal or prerenal failure.…”

Punica granatum seed oil detracts peritoneal adhesion: Perusing antioxidant, anti‐inflammatory, antifibrotic, and antiangiogenic impacts

“…Following our results, Yayla and co‐workers figured out that PSO (0.32 and 0.64 ml/kg, i.p.) attenuated the MDA and TNF‐α levels as well as NADPH oxidase activity while augmented superoxide dismutase (SOD) activity and GSH level following the ovarian ischemia/reperfusion injury in rats (Yayla et al, 2018). Similarly, a nano‐droplet formulation of pomegranate seed oil suppressed MDA and oxidative stress levels in brain tissue of experimental autoimmune encephalomyelitis (EAE) mice as an established model of multiple sclerosis (MS) (Binyamin et al, 2015).…”

Effect of Cadmium chloride on the Liver, kidney and heart tissues and the protective role of pomegranate seed oil in male Albino rats.

“…In several studies, PSO administration led to significant reduction of Malondialdehyde (MDA) concentration in tissue homogenate samples [30], [31]. Low dose PSO application reduced significantly oxidative stress and NADPH oxidase activity in both ischemic at the same time, low-dose PSO extract reduced TNF-α levels and significantly increased antioxidant activity [32].…”