Protective Effects of Poly (butyl) Cyanoacrylate Nanoparticles Containing Vasoactive Intestinal Peptide Against 6-Hydroxydopamine-Induced Neurotoxicity In Vitro

Xu, Zhiran; Wang, Wu-Fang; Liang, Xin-Fang; Liu, Zehua; Liu, Yu; Lin, Liang; Zhu, Xuan

doi:10.1007/s12031-014-0438-9

Cited by 9 publications

(4 citation statements)

References 40 publications

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“…Several factors contribute to the high entrapment efficiency of propolis (near 90%) into the PBCA-NP, including high solid-state solubility of drug/substance in the polymer, the solubility of drugs in the aqueous phase, and the fast rate of precipitation of the polymer in the organic phase. Additionally in this experiment, polymer–surfactant aggregates might be formed at higher surfactant concentrations minimizing the solubility of the drug into the aqueous phase ( Xu et al, 2015 ). Studies of propolis release kinetics at different pH values in PBS, showed an initial burst release within the first 6 h, which may be associated with the distribution of a small amount of propolis from the surface of the PBCA-NP.…”

Section: Discussionmentioning

confidence: 99%

Targeted Propolis-Loaded Poly (Butyl) Cyanoacrylate Nanoparticles: An Alternative Drug Delivery Tool for the Treatment of Cryptococcal Meningitis

et al. 2021

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In this study, we describe a nano-carrier system for propolis that is able to cross an in vitro model of the blood-brain barrier (BBB) and effectively reduce the virulence of Cryptococcus neoformans in animal models. Antimicrobial properties of propolis have been widely studied. However, propolis applications are limited by its low water solubility and poor bioavailability. Therefore, we recently formulated novel poly (n-butyl cyanoacrylate) nanoparticles (PBCA-NP) containing propolis. PBCA-NP are biocompatible, biodegradable and have been shown to effectively cross the BBB using apolipoprotein E (ApoE) as a ligand. Prepared nanoparticles were characterized for particle size, zeta potential, propolis entrapment efficiency and in vitro release. Additionally, the PBCA-NP were functionalized with polysorbate 80, which then specifically adsorbs ApoE. Using an in vitro BBB model of human brain microvascular endothelial cells hCMEC/D3, it was shown that fluorescence labelled ApoE-functionalized PBCA-NP were internalized by the cells and translocated across the cell monolayer. Propolis-loaded PBCA-NP had in vitro, antifungal activity against C. neoformans, which causes meningitis. To utilize the invertebrate model, Galleria mellonella larvae were infected with C. neoformans and treated with propolis-loaded PBCA-NP. The larvae exhibited normal behavior in toxicity testing, and treatment with propolis-loaded PBCA-NP increased survival in the C. neoformans-infected larvae group. In addition, following cryptococcal infection and then 7 days of treatment, the tissue fungal burden of mice treated with propolis-loaded PBCA-NP was significantly lower than control groups. Therefore, our ApoE-functionalized propolis-loaded PBCA-NP can be deemed as a potential targeted nanoparticle in the therapeutic treatment of cerebral cryptococcosis.

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Section: Discussionmentioning

confidence: 99%

Targeted Propolis-Loaded Poly (Butyl) Cyanoacrylate Nanoparticles: An Alternative Drug Delivery Tool for the Treatment of Cryptococcal Meningitis

et al. 2021

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“…In our previous study, polybutylcyanoacrylate nanoparticles (PBCA NP) was used as a polymeric colloidal vector for brain-derived neurotrophic factor (BDNF) gene transfection and BDNF protein delivery; uptake of these agents by mouse induced pluripotent stem cells (iPSCs) resulted in neural-lineage-committed cells in as short as 7 days [15,16]. The biocompatible and biodegradable nature of PBCA NPs, as well as central nervous system targeting capabilities, conferred by polysorbate 80 (PS80) coating, makes it ideal as a delivery system for drugs, peptides, proteins, and genes into the central nervous system [17][18][19]. PBCA NPs can be synthesized under ambient conditions using scalable emulsion polymerization of n-butylcyanoacrylate monomers or nano-precipitation of pre-formed PBCA polymers [20], and the cargo can either be carried by surface adsorption on pre-formed nanoparticles or be encapsulated within the nanoparticle during its synthetic process.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Optimization of Chitosan-Coated Polybutylcyanoacrylate Nanoparticles for the Transfection-Guided Neural Differentiation of Mouse Induced Pluripotent Stem Cells

Lin

Lai

Chang

et al. 2021

IJMS

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Gene transfection is a valuable tool for analyzing gene regulation and function, and providing an avenue for the genetic engineering of cells for therapeutic purposes. Though efficient, the potential concerns over viral vectors for gene transfection has led to research in non-viral alternatives. Cationic polyplexes such as those synthesized from chitosan offer distinct advantages such as enhanced polyplex stability, cellular uptake, endo-lysosomal escape, and release, but are limited by the poor solubility and viscosity of chitosan. In this study, the easily synthesized biocompatible and biodegradable polymeric polysorbate 80 polybutylcyanoacrylate nanoparticles (PS80 PBCA NP) are utilized as the backbone for surface modification with chitosan, in order to address the synthetic issues faced when using chitosan alone as a carrier. Plasmid DNA (pDNA) containing the brain-derived neurotrophic factor (BDNF) gene coupled to a hypoxia-responsive element and the cytomegalovirus promotor gene was selected as the genetic cargo for the in vitro transfection-guided neural-lineage specification of mouse induced pluripotent stem cells (iPSCs), which were assessed by immunofluorescence staining. The chitosan-coated PS80 PBCA NP/BDNF pDNA polyplex measured 163.8 ± 1.8 nm and zeta potential measured −34.8 ± 1.8 mV with 0.01% (w/v) high molecular weight chitosan (HMWC); the pDNA loading efficiency reached 90% at a nanoparticle to pDNA weight ratio of 15, which also corresponded to enhanced polyplex stability on the DNA stability assay. The HMWC-PS80 PBCA NP/BDNF pDNA polyplex was non-toxic to mouse iPSCs for up to 80 μg/mL (weight ratio = 40) and enhanced the expression of BDNF when compared with PS80 PBCA NP/BDNF pDNA polyplex. Evidence for neural-lineage specification of mouse iPSCs was observed by an increased expression of nestin, neurofilament heavy polypeptide, and beta III tubulin, and the effects appeared superior when transfection was performed with the chitosan-coated formulation. This study illustrates the versatility of the PS80 PBCA NP and that surface decoration with chitosan enabled this delivery platform to be used for the transfection-guided differentiation of mouse iPSCs.

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“…The tween 80 (T80)-coated polybutylcyanoacrylate nanoparticle (PBCA NP) is a versatile polymeric delivery system that exhibits CNS-targeting capabilities [31,32], and it has been widely studied for the delivery of various substances, including drugs, proteins, and peptides, and genes that are normally impermeable to the BBB into the CNS [33,34,35,36]. The cargos can either be carried by surface adsorption or encapsulation using the easily scalable polymerization or nano-precipitating methods under ambient conditions with the n-butylcyanoacrylate (BCA) monomer or pre-formed PBCA polymers, respectively [37].…”

Section: Introductionmentioning

confidence: 99%

Comparison between Polybutylcyanoacrylate Nanoparticles with Either Surface-Adsorbed or Encapsulated Brain-Derived Neurotrophic Factor on the Neural Differentiation of iPSCs

Lin

Chung

Chen

et al. 2019

IJMS

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The brain-derived neurotrophic factor (BDNF) is vital in the neural differentiation of neural stem/progenitor cells, and together may have therapeutic potential for neural regeneration. In this study, a multiplexed polybutylcyanoacrylate nanoparticle (PBCA NP) delivery platform was constructed, incorporating either surface-adsorbed or encapsulated BDNF for the induction of neural differentiation in induced pleuripotent stem cells (iPSCs), where tween 80 (T80) and superparamagnetic iron oxide (SPIO) were added for central nervous system (CNS) targeting and magnetic resonance (MR) image tracking, respectively. Both methods by which the BDNF was carried resulted in loading efficiencies greater than 95%. The nanoparticle-mediated delivery of BDNF resulted in neural differentiation of iPSCs detected on immunofluorescence staining as early as 7 days, with enhanced differentiation efficiency by 1.3-fold compared to the control on flow cytometry; the delivery system of surface-adsorbed BDNF gave rise to cells that had the best neural development than the encapsulated formulation. T80-coating disrupted the in vitro blood–brain barrier model with a corresponding 1.5- to two-fold increase in permeability. SPIO-loaded PBCA NPs exhibited a concentration-dependent, rapid decay in signal intensity on the phantom MR experiment. This study demonstrates the versatility of the PBCA NP, and the surface-adsorption of BDNF is the preferred method of delivery for the differentiation of iPSCs.

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Protective Effects of Poly (butyl) Cyanoacrylate Nanoparticles Containing Vasoactive Intestinal Peptide Against 6-Hydroxydopamine-Induced Neurotoxicity In Vitro

Cited by 9 publications

References 40 publications

Targeted Propolis-Loaded Poly (Butyl) Cyanoacrylate Nanoparticles: An Alternative Drug Delivery Tool for the Treatment of Cryptococcal Meningitis

Targeted Propolis-Loaded Poly (Butyl) Cyanoacrylate Nanoparticles: An Alternative Drug Delivery Tool for the Treatment of Cryptococcal Meningitis

Characterization and Optimization of Chitosan-Coated Polybutylcyanoacrylate Nanoparticles for the Transfection-Guided Neural Differentiation of Mouse Induced Pluripotent Stem Cells

Comparison between Polybutylcyanoacrylate Nanoparticles with Either Surface-Adsorbed or Encapsulated Brain-Derived Neurotrophic Factor on the Neural Differentiation of iPSCs

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