Protective effects of orange (Citrus sinensis L.) peel aqueous extract and hesperidin on oxidative stress and peptic ulcer induced by alcohol in rat

Abstract

BackgroundMassive alcohol drinking can lead to gastric ulcer. In the present study we investigated the gastroprotective effect of Citrus sinensis peel aqueous extract (CSPE) and Hesperidin (H) in ethanol (EtOH) induced oxidative stress and peptic ulcer in rats.MethodsSeventy adult male Wistar rats were divided into seven groups of 10 each: control, EtOH (4 g/kg b.w.), EtOH + various doses of CSPE (100, 200 and 400 mg/kg, b.w.), EtOH + Hesperidin (50 mg/kg, p.o.) and EtOH + Omeprazole (OM, 20 mg/kg, p.o.). Animals were perorally (p.o.) pre-treated with CSPE during 15 days and intoxicated with a single oral administration of EtOH (4 g/kg b.w.) during 2 h. Gastric ulcer was induced in rats with a single dose of ethanol (EtOH). Ulcer index, gene expression of gastric cyclooxygenase-2 (COX-2), tumor necrosis factor alpha (TNF-α), malondialdhyde (MDA), hydrogen peroxide H2O2 and Thiol groups (−SH) content in stomach and antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and gluthation peroxidise (GPx) were measured. Furthermore, histopathological examinations were performed.ResultsThe results showed that ethanol induced gastric damage, improving oxidative stress markers level such as MDA (121 ± 4.45 nmol/mg proteins) and H2O2 (24.62 ± 1.04 μmol/mg proteins), increased pro-inflammatory cytokine (TNF-α level), as well as the expression of COX-2 in the ethanol group. However, a significant depletion of enzymatic and non-enzymatic antioxidants were observed, such as, GPx (72%), SOD (57.5%), CAT (41.6%) and -SH (50%). The lesions were associated with severe histopathological damage. The both Citrus sinensis peel aqueous extract (CSPE) and hesperidin significantly protect against all gastric damages caused by ethanol administration in rats.ConclusionsWe propose that CSPE and hesperidin exhibit protective effects in EtOH-induced peptic ulcer in rat. This protection might be related in to part its antioxidant properties as well as its opposite effects on some studied intracellular mediators.

RETRACTED: Antiulcerogenic Activity of Li‐Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR‐2/MyD88 Signaling Pathway

“…Duodenal ulcer (DU) is one of the major gastrointestinal disorders, which affects annually approximately 10-15% of the population worldwide [1]. DU occurs due to loss of balance between offensive and defensive factors.…”

“…Recently, serious attention has been paid to natural products, owing not only to its favorable safety profile and relatively low cost but also to its attracting efficacy, minimal postprandial untoward effects, and superior compatibility with human body system. With regard to DU therapies, various research studies have shown potential gastroprotective activities of plant-based extracts and TCM formulas, such as Citrus sinensis [1], Melastoma malabathricum [4], Spondias mombin [5], and Xiao Chaihu decoction [6].…”

Orange peel fragment improves antioxidant capacity and haematological profile of Nile tilapia subjected to heat/dissolved oxygen‐induced stress

“…One of them is the upregulation of the Nrf2 gene expression which enhances antioxidant enzymes activity, thus decreasing oxidative stress (Parhiz, Roohbakhsh, Soltani, Rezaee, & Iranshahi, ). The positive antioxidant capacity of hesperidin had been previously described for production animals, such as poultry (orange peel 0.04%—Akbarian, Golian, Kermanshahi, Smet, & Michiels, ; hesperidin 0.002%—Ognik, Cholewińska, Sembratowicz, Grela, & Czech, ) and laying hens (0.05% hesperidin—Iskender et al, ), and also for rats (0.04% orange peel aqueous extract—Selmi, Rtibi, Grami, Sebai, & Marzouki, ). Further studies assessing hesperidin, reactive oxygen metabolites and the biological antioxidant potential could help in understanding OPF on Nile tilapia antioxidant capacity.…”

Evidence of insulin-dependent signalling mechanisms produced by Citrus sinensis (L.) Osbeck fruit peel in an insulin resistant diabetic animal model

“…Citrus sinensis (L.) Osbeck (Rutaceae), commonly known as the sweet orange, is ubiquitous in human diet across the world (Lv et al, 2015) and belongs to the citrus family (Duarte et al, 2016). C. sinensis peel is a rich source of phenolic compounds, with antidiabetic, antioxidant, and free radical scavenging activities, effects against gastrointestinal illnesses, and potential protection against different cancers and cardiovascular disorders (Rotelli et al, 2003;Selmi et al, 2017). A number of recent experiments have explored the therapeutic potential of phenolics derived from citrus species as a single bioactive molecule (Selmi et al, 2017).…”

“…C. sinensis peel is a rich source of phenolic compounds, with antidiabetic, antioxidant, and free radical scavenging activities, effects against gastrointestinal illnesses, and potential protection against different cancers and cardiovascular disorders (Rotelli et al, 2003;Selmi et al, 2017). A number of recent experiments have explored the therapeutic potential of phenolics derived from citrus species as a single bioactive molecule (Selmi et al, 2017). C. sinensis peel exhibits hypoglycaemic and hypolipidaemic effects in an alloxan-induced animal model of type 1 diabetes (Luka et al, 2017).…”