Protective effects of N-acetylcysteine on myocardial injury induced by hindlimb ischaemia–reperfusion: a histological study in a rat model

Takhtfooladi, Mohammad Ashrafzadeh; Jahanshahi, Gholamreza; Sotoudeh, Ahmad; Jahanshahi, Amirali

doi:10.1007/s00580-013-1768-7

Cited by 2 publications

(2 citation statements)

References 29 publications

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“…The analysis of the myocardium under light microscopy revealed the presence of more edema, neutrophil infiltration, and coagulative necrosis in Group II than in Group III; this shows tramadol’s tendency to attenuate these injuries, a trend that has statistical significance. This observation was supported by Takhtfooladi et al 27 , who demonstrated that temporary occlusion of the femoral artery in rats resulted in histological changes.…”

Section: Discussionmentioning

confidence: 56%

“…As far as we know, there are only a few reports demonstrating remote myocardial injury following skeletal muscle IR injury. 8 The results of Takhtfooladi et al 27 indicated that hindlimb IR induces severe myocardial damage and that N-acetylcysteine has protective effects on the myocardium after hindlimb IR. Their data supported the concept that temporary occlusion of the femoral artery induced myocardial injury in rats 27 .…”

Section: Discussionmentioning

confidence: 99%

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Tramadol Alleviates Myocardial Injury Induced by Acute Hindlimb Ischemia Reperfusion in Rats

Takhtfooladi¹,

Asl²,

Shahzamani³

et al. 2015

Arquivos Brasileiros De Cardiologia

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BackgroundOrgan injury occurs not only during periods of ischemia but also during reperfusion. It is known that ischemia reperfusion (IR) causes both remote organ and local injuries.ObjectiveThis study evaluated the effects of tramadol on the heart as a remote organ after acute hindlimb IR.MethodsThirty healthy mature male Wistar rats were allocated randomly into three groups: Group I (sham), Group II (IR), and Group III (IR + tramadol). Ischemia was induced in anesthetized rats by left femoral artery clamping for 3 h, followed by 3 h of reperfusion. Tramadol (20 mg/kg, intravenous) was administered immediately prior to reperfusion. At the end of the reperfusion, animals were euthanized, and hearts were harvested for histological and biochemical examination.ResultsThe levels of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were higher in Groups I and III than those in Group II (p < 0.05). In comparison with other groups, tissue malondialdehyde (MDA) levels in Group II were significantly increased (p < 0.05), and this increase was prevented by tramadol. Histopathological changes, including microscopic bleeding, edema, neutrophil infiltration, and necrosis, were scored. The total injuryscore in Group III was significantly decreased (p < 0.05) compared with Group II.ConclusionFrom the histological and biochemical perspectives, treatment with tramadol alleviated the myocardial injuries induced by skeletal muscle IR in this experimental model.

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Tramadol Alleviates Myocardial Injury Induced by Acute Hindlimb Ischemia Reperfusion in Rats

Takhtfooladi¹,

Asl²,

Shahzamani³

et al. 2015

Arquivos Brasileiros De Cardiologia

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Investigation of Proteus vulgaris and Elizabethkingia meningoseptica invasion on muscle oxidative stress and autophagy in Chinese soft-shelled turtle (Pelodiscus sinensis)

Bao

Deng³

et al. 2021

Sci Rep

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Muscle is an important structural tissue in aquatic animals and it is susceptible to bacterial and fungal infection, which could affect flesh quality and health. In this study, Chinese soft-shelled turtles were artificially infected with two pathogens, Proteus vulgaris and Elizabethkingia meningoseptica and the effects on muscle nutritional characteristics, oxidative stress and autophagy were assayed. Upon infection, the muscle nutritional composition and muscle fiber structure were notably influenced. Meanwhile, the mRNA expression of Nrf2 was down-regulated and Keap1 up-regulated, thus resulting in a decrease in antioxidant capacity and oxidative stress. However, with N-acetylcysteine treatment, the level of oxidative stress was decreased, accompanied by significant increases in antioxidant enzyme activities and the mRNA levels of SOD, CAT, GSTCD, and GSTO1. Interestingly, there was a significant increase in autophagy in the muscle tissue after the pathogen infection, but this increase could be reduced by N-acetylcysteine treatment. Our findings suggest that muscle nutritional characteristics were dramatically changed after pathogen infection, and oxidative stress and autophagy were induced by pathogen infection. However, N-acetylcysteine treatment could compromise the process perhaps by decreasing the ROS level and regulating Nrf2-antioxidant signaling pathways.

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Protective effects of N-acetylcysteine on myocardial injury induced by hindlimb ischaemia–reperfusion: a histological study in a rat model

Cited by 2 publications

References 29 publications

Tramadol Alleviates Myocardial Injury Induced by Acute Hindlimb Ischemia Reperfusion in Rats

Tramadol Alleviates Myocardial Injury Induced by Acute Hindlimb Ischemia Reperfusion in Rats

Investigation of Proteus vulgaris and Elizabethkingia meningoseptica invasion on muscle oxidative stress and autophagy in Chinese soft-shelled turtle (Pelodiscus sinensis)

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