Protective effects of molsidomine against doxorubicin-induced renal damage in rats

Oğuz, Fatih; Beytur, Ali; Sarihan, Ediz; Oguz, Hilal K; Bentli, Recep; Şamdancı, Emine; Köse, Evren; Polat, Alaaddin; Duran, Zeynep Rumeysa; Parlakpınar, Hakan; Ekinci, Nihat

doi:10.25011/cim.v39i1.26325

Cited by 7 publications

(5 citation statements)

References 38 publications

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“…In this study, BAs reduced the increased MDA levels and significantly enhanced GSH level in a dosedependent manner versus DXR group. The enhanced GSH level in BAs-treated mice may partially explain its nephroprotective effect as GSH provides a thiol group triggering detoxification processes of glutathione peroxidase [51]. Moreover, co-treatment with BAs diminished peroxidation of lipids in the kidney tissue with significant protection of tubular structures against DXRinduced injury.…”

Section: Discussionmentioning

confidence: 98%

Boswellic acids ameliorate doxorubicin-induced nephrotoxicity in mice: a focus on antioxidant and antiapoptotic effects

Sami

Ali

Galhom

et al. 2019

Egyptian Journal of Basic and Applied Sciences

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Doxorubicin (DXR) is a broad-spectrum anti-cancer. Doxorubicin irreversible toxicity resulting from oxidative damage limits its therapeutic use. Boswellic acids (BAs) are inhibitors of 5-lipoxygenase and have been used in traditional medicine for their powerful anti-inflammatory effects and cellular protective effects. This study tested the protecting mechanisms of BAs against nephrotoxicity induced by DXR in mice and explored their antioxidant and antiapoptotic activities in the form of immunohistochemical expression of Bcl2 in the tissue of the kidney. DXR (6 mg/kg) was injected intraperitoneally weekly to mice along with BAs (125, 250 and 500 mg/kg) daily. The experiment continued for three weeks. It was found that the elevated serum urea and creatinine in DXR-treated mice were ameliorated by BAs. Furthermore, BAs decreased malondialdehyde and increased glutathione levels in renal tissues of DXR-treated mice. The immunostained kidney tissue showed an antiapoptotic effect for BAs as it increased expression of Bcl2 in mice co-treated with BAs with DXR compared to the DXR control mice. Western blot analysis demonstrated that DXR control group showed greater expression for renal caspase 3 and mice administered BAs (125-500 mg/kg) along with DXR showed significant downregulations. These findings were supported by the DNA laddering assay and histopathological examination of renal tissues stained with haematoxylin+eosin or periodic acid Schiff. Results suggest BAs for nephroprotection against toxicity induced by DXR.

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Section: Discussionmentioning

confidence: 98%

Boswellic acids ameliorate doxorubicin-induced nephrotoxicity in mice: a focus on antioxidant and antiapoptotic effects

Sami

Ali

Galhom

et al. 2019

Egyptian Journal of Basic and Applied Sciences

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“…MDA content is an important parameter to reflect the potential antioxidant capacity, which can reflect the rate and intensity of lipid peroxidation, and also indirectly reflect the degree of tissue peroxidation damage. This process can induce kidney injury and promote its development (Oguz et al 2016). In order to maintain the stability of the internal environment in animals, a balance between oxidation and antioxidant substances is needed (Olga et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Florfenicol induces renal toxicity in chicks by promoting oxidative stress and apoptosis

Wang

Han

Cui

et al. 2020

Environ Sci Pollut Res

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To explore the mechanism of renal toxicity induced by florfenicol (FFC), 120 chicks were randomly divided into 6 groups, 20 in each group. Except for the control group, different doses of FFC (0.15 g/L, 0.3 g/L, 0.6 g/L, 1.2 g/L, and 1.8 g/L) were added to drinking water in the other 5 groups. Five days later, blood was collected from the vein under the wing, and the complete kidneys were obtained as soon as possible, then tested the experimental indicators. The results showed that compared with control group, all doses of FFC significantly reduced the average weight gain of chicks (P < 0.05 or P < 0.01). Except for the 0.15 g/L FFC group, kidney index of chicks in the other doses of FFC groups were significantly increased (P < 0.05 or P < 0.01). The kidney tissues in all FFC groups showed obvious damage, deformities, cell atrophy, and cell gap enlargement. In addition, all doses of FFC significantly increased the contents of uric acid (UA), blood urea nitrogen (BUN), creatinine (CRE) in serum, and malondialdehyde (MDA) in renal tissue (P < 0.05 or P < 0.01), but significantly reduced the levels of glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) in renal tissue (P < 0.05 or P < 0.01). FFC significantly inhibited the mRNA and protein expression levels of nuclear factor-erythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), and nicotinamide adenine dinucleotide phosphate: quinone oxidoreductase-1 (NQO-1), and increased the mRNA and protein expression levels of p53, Caspase-3, and Caspase-6 (P < 0.05 or P < 0.01). The apoptotic rate of renal cells in all doses of FFC groups increased significantly (P < 0.05 or P < 0.01). It was concluded that FFC had a certain degree of nephrotoxicity, and with the increase of FFC concentration, the kidney injury of chicks became more and more serious. FFC promoted oxidative stress response in kidney of chicks by inhibiting the expression of related factors in Nrf2-ARE pathway. Moreover, the expression of pro-apoptotic factors was upregulated to improve the apoptosis rate of renal cells, which resulted in excessive apoptosis of renal cells and seriously affected the kidney function of chicks.

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“…In earlier investigations, we showed that treatment with DOX results in low levels of these enzymes or non-enzymatic antioxidants. Thus, rats treated with DOX exhibited lower levels of SOD and CAT than the untreated controls, according to Oguz et al (2016). In addition, when free radicals grow, SOD and CAT activity, which protect the cell membrane and its contents, decreases significantly and oxidative stress results in low levels of SOD and CAT, increasing the amount of H2O2 in cells (Ighodaro & Akinloye, 2018).…”

Section: Discussionmentioning

confidence: 96%

“…In addition, when free radicals grow, SOD and CAT activity, which protect the cell membrane and its contents, decreases significantly and oxidative stress results in low levels of SOD and CAT, increasing the amount of H2O2 in cells (Ighodaro & Akinloye, 2018). Toxins are mostly eliminated and reduced in cells affected by GSH and low levels of GSH weaken the body's ability to fight against ROS (Oguz et al, 2016). It has been hypothesized that there is a relationship between GPx and GSH, because earlier studies have shown that decreased GPx activity could have played a role in determining the availability of GSH in comparison to the DOX-treated group and control groups (Shabalala et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Untitled

2023

Pak. J. Pharm. Sci.,

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The goal of this research was to determine whether the combination of baicalein (BL) and losartan (LT) would provide greater protection against DOX-induced nephrotoxicity. There were five groups of male rats in the experiment: the 1) control, 2) DOX, 3) DOX+LT, 4) DOX+BL and 5) DOX+LT+BL groups. A dose of DOX was administered following two weeks of LT and BL therapy. In the DOX-affected group, serum renal indicators, including creatinine and urea, rose considerably compared to those in the control groups (p<0.01). Further, there was a statistically significant increase (p<0.001) in the levels of the cytokines that promote inflammation in renal tissue, including tumor necrosis factor-α, interleukin (IL)-1 and IL-6. In addition, the level of the anti-inflammatory cytokine IL-10 decreased significantly (p<0.001) in the DOX-challenged group compared to the control groups. In addition, renal cell indicators of oxidative stress (p<0.001) and enzymatic activity (p<0.01) reduced dramatically in the DOX-challenged group, whereas renal cell thiobarbituric acid retroactive materials rose greatly (p<0.001). Finally, the DOX group had higher kidney protein expression and inflammatory activity than the control groups (p<0.001). The combination of BL and LT therapy protected DOX-challenged rats via antioxidant and anti-inflammatory activities.

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Protective effects of molsidomine against doxorubicin-induced renal damage in rats

Cited by 7 publications

References 38 publications

Boswellic acids ameliorate doxorubicin-induced nephrotoxicity in mice: a focus on antioxidant and antiapoptotic effects

Boswellic acids ameliorate doxorubicin-induced nephrotoxicity in mice: a focus on antioxidant and antiapoptotic effects

Florfenicol induces renal toxicity in chicks by promoting oxidative stress and apoptosis

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