Protective effects of mannan in Caco-2/TC7 cells treated with wheat-derived peptides

Vincentini, Olimpia; Angelis, De; Iannuccelli, Roberta; Silano, Marco; Stammati, Annalaura; Vincenzi, Massimo De

doi:10.1016/j.carbpol.2005.08.008

Cited by 4 publications

(2 citation statements)

References 34 publications

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“…[127,128] Buckwheat (fagopyrum esculentum) buckwheat PI 5 to 7 kDa, BB class inhibition of leukemia cells [129] rice bran agglutinin 36 kDa, 2 identical 18 kDa-subunits, each with of 2 chains (10/8 kDa) growth arrest of human monoblastic leukemia U937 cells by G2/M phase cell cycle arrest [130] wheat germ agglutinin 26 kDa lectin apoptosis/necrosis in human leukaem. cells+PBMC [131,132] gliadine-derived peptides various peptides in peptic-tryptic digest inhibition of Caco-2 cell DNA/RNA synthesis [133,134] Wheat and other graminae gamma-thionins, defensins defensins from wheat, barley, ~5 kDa, homologous to viscotoxins inhibition of growth of different mammalian cells [103,135] Abbreviations: (AA) amino acid(s); (BB) Bowman-Birk; (PI) protease inhibitor; (RIP) ribosome inactivating protein.…”

Section: Plant Defensins Gamma-thioninsmentioning

confidence: 99%

Cytotoxic and Allergenic Potential of Bioactive Proteins and Peptides

Hartmann¹,

Wal²,

Bernard³

et al. 2007

CPD

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This review article deals with the assessment of cytotoxic and allergenic potential of bioactive proteins and peptides. It is evident that 'novel' foods or nutraceuticals containing bioactive proteins and peptides must fulfill their proposed "health claim". Furthermore, there is a need to assess their potential to exert adverse effects before they can be made widely available to consumers. A brief overview of compounds (i.e. proteins and peptides of animal and plant origin) and mechanisms involved in cytotoxic and allergenic (adverse) reactions is given along with some recent results obtained from ongoing studies. There are numerous proteins and peptides of plant and animal origin that are known to exhibit cytotoxic effects. There is evidence that many cytotoxic compounds described in the literature exclusively affect malignant cells leading to the assumption that a cancer protective effect could exist for such bioactive proteins and peptides. All the constituents that are responsible for the allergenicity of foods (as well as of pollens) are proteinaceous in nature. Some protein breakdown products, i.e. peptide fragments, may conserve part of the allergenicity of the native protein and thus can also be considered as allergens. The molecular basis of IgE recognition underlying cow's milk protein allergy is described. Some results from studies on volunteers fed caseinophosphopeptides or potentially hypotensive milk protein hydrolysates illustrate the major difference between allergenicity and immunogenicity. The data presented on the relationship between the structure of food proteins and peptides and their allergenicity shows the difficulty in trying to assess the "non-allergenicity" of products derived from an allergenic source, even if the process used involved extensive hydrolysis of the native protein(s). A 'weight of evidence approach' for assessing the potential allergenicity of a novel protein with no history of prior allergenicity is also presented with regard to the current EU Regulations.

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Section: Plant Defensins Gamma-thioninsmentioning

confidence: 99%

Cytotoxic and Allergenic Potential of Bioactive Proteins and Peptides

Hartmann¹,

Wal²,

Bernard³

et al. 2007

CPD

View full text Add to dashboard Cite

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“…For example, the disaccharide (di-N-acetylchitobiose) and the trisaccharide (tri-N-acetylchitotriose) are promising candidates for preventing celiac disease, which is caused by gliadin-derived peptides. 1) The hexasaccharide (hexa-N-acetylchitohexaose) exhibits antitumor [2][3][4] and antimicrobial activity 5,6) in mice by enhancing immunological defenses. Moreover, several heterodisaccharides consisting of D-galactopyranose (Gal) and GlcNAc have shown physiologically beneficial functions.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic synthesis of novel oligosaccharides from N-acetylsucrosamine and melibiose using Aspergillus niger α-galactosidase, and properties of the products

Sakaki

Tashiro

Katou

et al. 2016

Bioscience, Biotechnology, and Biochemistry

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Two kinds of oligosaccharides, N-acetylraffinosamine (RafNAc) and N-acetylplanteosamine (PlaNAc), were synthesized from N-acetylsucrosamine and melibiose using the transgalactosylation activity of Aspergillus niger α-galactosidase. RafNAc and PlaNAc are novel trisaccharides in which d-glucopyranose residues in raffinose (Raf) and planteose are replaced with N-acetyl-d-glucosamine. These trisaccharides were more stable in acidic solution than Raf. RafNAc was hydrolyzed more rapidly than Raf by α-galactosidase of green coffee bean. In contrast, RafNAc was not hydrolyzed by Saccharomyces cerevisiae invertase, although Raf was hydrolyzed well by this enzyme. These results indicate that the physicochemical properties and steric structure of RafNAc differ considerably from those of Raf.

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Saccharification of β-Chitin From Squid Pen by a Fermentation Method Using Recombinant Chitinase-Secreting Escherichia coli

Minamoto

Takahashi

Kitahara

et al. 2015

Appl Biochem Biotechnol

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Two strains [BL21(DE3) and HMS174(DE3)] of Escherichia coli harboring the recombinant chitinase expression plasmid pVP-Chi, which contains Vibrio parahaemolyticus chitinase gene with an attached signal sequence, were prepared. These E. coli transformants produced a large amount of recombinant chitinase, which hydrolyzes chitin to yield di-N-acetylchitobiose (GlcNAc)2, under the presence of isopropyl-1-thio-β-D-galactopyranoside (IPTG), and secreted the enzyme into their culture fluid with the aid of the signal peptide. Cultivation of these E. coli transformants in Luria-Bertani medium containing squid pen β-chitin and IPTG gave rise to the decomposition of this polysaccharide and the accumulation of (GlcNAc)2 in the culture fluid. Through these experiments, we confirmed that the use of strain HMS174(DE3) was preferable for the stable accumulation of (GlcNAc)2 in the culture fluid during cultivation owing to lower (GlcNAc)2 assimilation compared to BL21(DE3). Next, using E. coli HMS174(DE3) transformants, we conducted saccharification of different forms (fluffy fiber, flake, and powder) of β-chitin samples prepared from squid pens in Bacterion-N-KS(B)K medium containing 2 % of each sample under the presence of IPTG. In these experiments, (GlcNAc)2 was isolated with a more than 20 % stoichiometric yield from each culture supernatant through charcoal column chromatography followed by recrystallization.

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Protective effects of mannan in Caco-2/TC7 cells treated with wheat-derived peptides

Cited by 4 publications

References 34 publications

Cytotoxic and Allergenic Potential of Bioactive Proteins and Peptides

Cytotoxic and Allergenic Potential of Bioactive Proteins and Peptides

Enzymatic synthesis of novel oligosaccharides from N-acetylsucrosamine and melibiose using Aspergillus niger α-galactosidase, and properties of the products

Saccharification of β-Chitin From Squid Pen by a Fermentation Method Using Recombinant Chitinase-Secreting Escherichia coli

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