Protective Effects of Lactobacillus rhamnosus GG on Aflatoxins-Induced Toxicities in Male Albino Mice

Deabes, Mohamed M.; Darwish, Hassan R.; B, Abdel-Aziz K; Farag, Ibrahim M.; Sa, Nada; Tawfek, Nagy

doi:10.4172/2161-0525.1000132

Cited by 10 publications

(3 citation statements)

References 74 publications

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“…HPLC (Agilent 1100 series) equipped with a fluorescence detector (G 1321A) analysis was carried out with a liquid chromatograph equipped with solvent delivery systems (Agilent Technologies, Inc. 200 Regency Forest Drive, Suite 330 Cary, NC 27511 USA) system containing a G1322A Vacuum Degasser, a G1312A binary pump and a reverse-phase analytical column packed with C 18 material (Agilent ZORBAX, DB-5 μm, 150 mm × 4.6 mm). The mobile phase consisted of water:acetonitril:methanol (240:120:40), according by Deabes et al [25], [26] Eshak et al [27], El-Soud et al [28], and Deabes et al [29 a,b ], for AFs G 1 , B 1 , G 2 , and B 2 , but for M1 the isocratic system with water:methanol:ACN 66:17:17. Separation was performed at 40°C temperature at a flow rate of 1.0 ml/min; the injection volume was 50 μl for both standard solutions and sample extracts by autosampler (G1329A).…”

Section: Hplc Conditionsmentioning

confidence: 99%

Aflatoxins Contamination of Human Food Commodities Collected from Jeddah Markets, Saudi Arabia

Tawila

Sadeq

Awad

et al. 2020

Open Access Maced J Med Sci

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BACKGROUND: Aflatoxins (AFs) are fungal secondary metabolites produced by Aspergillus flavus. They contaminate of dietary food with AFs is a worldwide problem that affects both food safety and agricultural economies. AIM: The aim of this study was designed to investigate the AFs contents of human food commodities mostly consumed in Jeddah, Saudi Arabia. METHODS: The study was designed in vitro, contents in six food categories. A total of 288 samples were collected from 78 different markets in Jeddah. AFs were determined by high-performance liquid chromatography with fluorescence detector using immunoaffinity column clean-up. RESULTS: The results indicated that the incidence rate 27.3% of nut samples collected from Jeddah, were contaminated with AFB1, AFB2. The concentrations of AFs (AFB1, AFB2, AFG1, and AFG2) were ranged from 0.19–482.4, 0.09–3.34, 0.19–87.1, to 0.09–579 μg/kg in the nut samples. CONCLUSION: The results demonstrate the importance of routine monitoring of AFs contamination in various dry foods for human consumed should be performed regularly and the nuts contained high levels of AFs. The legal regulations must be unauthorized for human consumption to control the health risks associated with AFs.

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Section: Hplc Conditionsmentioning

confidence: 99%

Aflatoxins Contamination of Human Food Commodities Collected from Jeddah Markets, Saudi Arabia

Tawila

Sadeq

Awad

et al. 2020

Open Access Maced J Med Sci

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“…A mobile phase including water/acetonitrile/mehanol in ratio 240:120:40 was used (18). Data observed were recorded with Millennium Chromatography Manager software (19).…”

Section: Determination Of Aflatoxinsmentioning

confidence: 99%

Extracellular Alkaline Lipase from a Novel Fungus Curvularia sp. DHE 5: Optimization of Physicochemical Parameters, Partial Purification and Characterization

El-Ghonemy

El-Gamal

Tantawy

et al. 2017

Food Technol. Biotechnol.

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SummaryThirty isolated fungal strains were screened for lipase production using Phenol Red plates, containing tributyrin as lipidic substrate, and a novel fungus identified genetically as Curvularia sp. DHE 5 was found as the most prominent strain. Various agro-industrial substrates were evaluated as inert supports for lipase production in solid-state fermentation. The highest yield of lipase ((83.4±2.2) U/g on dry mass basis) was reported with wheat bran medium after seven days of fermentation at pH=7.0, temperature of 30 °C, 70 % moisture content, inoculum size of 1.27·10 7 spore/mL and 2 % olive oil as an inducer. Supplementation of the medium with 0.05 % KCl as an ion source further increased lipase production to (88.9±1.2) U/g on dry mass basis. The enzyme was partially purified through ammonium sulphate fractionation (40 %) followed by dialysis, and its optimum pH and temperature were reported at 8.0 and 50 °C, respectively, with remarkable pH and thermal stability.

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“…This result suggested a beneficial role for L. rhamnosus GG upon dietary exposure to aflatoxin. Deabes et al (2012) evaluated whether L. rhamnosus GG could remove aflatoxin in vivo and showed that oral administration of L. rhamnosus GG at 1 × 10 cfu for 7 d to male albino mice significantly decreased aflatoxininduced toxicity (0.7 mg/kg of BW) by preventing oxidative stress, and by maintaining glutathione levels and superoxide dismutase activity. Another group assessed the activity of L. rhamnosus GG in vivo and demonstrated that rats fed aflatoxin B 1 (4.8 μmol/kg of BW) along with L. rhamnosus GG were safer from the hazardous effects of aflatoxin B 1 (Gratz et al, 2006).…”

Section: Removal Of Aflatoxin B 1 By L Rhamnosus Ggmentioning

confidence: 99%

Invited review: Microbe-mediated aflatoxin decontamination of dairy products and feeds

Kim

Lee

et al. 2017

Journal of Dairy Science

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Aspergillus flavus, Aspergillus parasiticus, and Aspergillus nomius contaminate corn, sorghum, rice, peanuts, tree nuts, figs, ginger, nutmeg, and milk. They produce aflatoxins, especially aflatoxin B, which is classified as a Group 1 carcinogen by the International Agency for Research on Cancer. Many studies have focused on aflatoxin removal from food or feed, especially via microbe-mediated mechanisms-either adsorption or degradation. Of the lactic acid bacteria, Lactobacillus rhamnosus GG efficiently binds aflatoxin B, and a peptidoglycan in the bacterium cell wall plays an important role. This ability of L. rhamnosus GG should be applied to the removal of aflatoxin B. Aflatoxin can be removed using other aflatoxin-degrading microorganisms, including bacterial and fungal strains. This review explores microbe-associated aflatoxin decontamination, which may be used to produce aflatoxin-free food or feed.

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Protective Effects of Lactobacillus rhamnosus GG on Aflatoxins-Induced Toxicities in Male Albino Mice

Cited by 10 publications

References 74 publications

Aflatoxins Contamination of Human Food Commodities Collected from Jeddah Markets, Saudi Arabia

Aflatoxins Contamination of Human Food Commodities Collected from Jeddah Markets, Saudi Arabia

Extracellular Alkaline Lipase from a Novel Fungus Curvularia sp. DHE 5: Optimization of Physicochemical Parameters, Partial Purification and Characterization

Invited review: Microbe-mediated aflatoxin decontamination of dairy products and feeds

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