Protective effects of L-carnitine, N-acetylcysteine and genistein in an experimental model of liver fibrosis

Demirören, Kaan; Doğan, Yaşar; Kocamaz, Halil; Ozercan, İbrahim Hanifi; İlhan, Selçuk; Üstündağ, Bilal; Bahçecıoğlu, İbrahim Halil

doi:10.1016/j.clinre.2013.08.014

Cited by 52 publications

(54 citation statements)

References 48 publications

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“…8). Conversely, liver sections from animals Values are given as mean ± SD (n = 6) a Significant compared with control at P < 0.05 b Significant compared with control at P < 0.01 c Significant compared with control at P < 0.001 The effects of CCl4 were prevented completely or partially by NAC antioxidant activity as it prevented the increase in lipid peroxidation products (Manibusan, Odin, & Eastmond, 2007;Morsy et al, 2012 andDemiroren et al, 2014). The development of CCl4-induced liver fibrosis is usually associated with oxidative stress and lipid peroxidation (Manibusan et al, 2007).…”

Section: Resultsmentioning

confidence: 99%

The protective effect of N-acetyl cysteine against carbon tetrachloride toxicity in rats

Foaud

Kamel

El-Monem

2018

JoBAZ

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Background: Carbon tetrachloride (CCL4) is used as a solvent for oils and fats, as a refrigerant, and as a dry-cleaning agent. Inhalation of its vapors can depress central nervous system activity and cause degeneration of the liver and kidneys. Aim: The aim of this study was to investigate whether N-acetyl cysteine (NAC) has a protective effect on the toxicity caused by carbon tetrachloride. Methods: Male rats were used in this experiment. Animals were divided into five groups, 5 animals each: G1 received olive oil, G2 was treated with CCL4 (1 ml/kg body wt) dissolved in olive oil (1:1), G3 received NAC (300 mg/kg body wt), G4 received CCL4 plus NAC, and G5 received NAC for 1 week then administrated to the same treatment of G4. All administrations were performed by gavage and maintained for 4 weeks. Results: The present study revealed that administration of CCL4 caused a significant increase in liver marker enzymes Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), and alkaline phosphatase (ALP); also, malondialdehyde (MDA) content in the liver tissue was increased. However, serum total protein showed no change in all groups. Also, histopathological investigation of CCL4 on the liver, testis, and kidney showed different deleterious effects. Additionally, CCl4 induced a highly significant increase in the percentage of sperm shape abnormality and sperm DNA tail moment values. Alternatively, result revealed that the two routes of NAC administrations caused a significant decrease in liver marker enzymes as well as MDA contents; improvement in liver, kidney, and testes architecture; a significant decrease in the percentage of sperm head abnormalities; and decline in the DNA tail moment values. Conclusion: The present study pointed out the protective effect of NAC against the toxicity of CCL4.

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Section: Resultsmentioning

confidence: 99%

The protective effect of N-acetyl cysteine against carbon tetrachloride toxicity in rats

Foaud

Kamel

El-Monem

2018

JoBAZ

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“…Several studies have shown that NAC could be used for the treatment of pulmonary fibrosis, liver fibrosis, and skin fibrosis [28][29][30][31] . In the present study, NAC inhibited the production of ECM proteins and the activation of TGF-β signaling pathway in the UUO kidneys.…”

Section: Wwwchinapharcom Shen Y Et Almentioning

confidence: 99%

N-acetylcysteine alleviates angiotensin II-mediated renal fibrosis in mouse obstructed kidneys

Shen

Miao

et al. 2016

Acta Pharmacol Sin

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Aim: To investigate the effects of ROS scavenger N-acetylcysteine (NAC) on angiotensin II (Ang II)-mediated renal fibrosis in vivo and in vitro. Methods: Mice were subjected to unilateral ureteral obstruction (UUO), and then treated with vehicle or NAC (250 mg/kg, ip) for 7 days. Histological changes of the obstructed kidneys were observed with Masson's trichrome staining. ROS levels were detected with DHE staining. The expression of relevant proteins in the obstructed kidneys was assessed using Western blotting assays. Cultured rat renal fibroblast NRK-49F cells were used for in vitro experiments. Results: In the obstructed kidneys, Ang II levels were significantly elevated, and collagen I was accumulated in the interstitial spaces. Furthermore, ROS production and the expression of p47 (a key subunit of NADPH oxidase complexes) were increased in a timedependent manner; the expression of fibronectin, α-SMA and TGF-β were upregulated. Administration of NAC significantly alleviated the fibrotic responses in the obstructed kidneys. In cultured NRK-49F cells, treatment with Ang II (0.001-10 μmol/L) increased the expression of fibronectin, collagen I, α-SMA and TGF-β in dose-dependent and time-dependent manners. Ang II also increased ROS production and the phosphorylation of Smad3. Pretreatment with NAC (5 μmol/L) blocked Ang II-induced oxidative stress and ECM production in the cells. Conclusion: In mouse obstructed kidneys, the fibrotic responses result from Ang II upregulation can be alleviated by the ROS scavenger N-acetylcysteine.

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“…The antioxidative capacity of N-acetyl-Lcysteine was also demonstrated in a model of chronic liver damage in rats (32). A study conducted in humans showed that the supplementation with N-acetyl-L-cysteine improved the oxidative status of patients after surgical treatments in the abdomen, which indicates the importance of the clinical application of this substance (33).…”

Section: Discussionmentioning

confidence: 92%

The Effects of Subchronic Methionine Overload Administered Alone or Simultaneously with L-cysteine or N-acetyl-L-cysteine on Body Weight, Homocysteine Levels and Biochemical Parameters in the Blood of Male Wistar Rats

Mićović

Stamenković

Nikolić

et al. 2016

Serbian Journal of Experimental and Clinical Research

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Hyperhomocysteinemia (HHC), both basal and after methionine load, may occur due to genetic disorders or defi ciencies of nutrients that aff ect the remethylation or transsulphuration pathways during methionine metabolism. HHC is involved in the pathogenesis of many illnesses as a SAŽETAK Hiperhomocisteinemija (HHC), kako bazalna tako i nakon ABBREVIATIONSAHDL -direct high-density cholesterol; ALB -albumin; ALP -alkaline phosphatase; ALT -alanine aminotransferase;AMY -amylase; AST -aspartate aminotransferase;bw -body weight BUN -blood urea nitrogen;Ca -calcium; Cl -chloride; CHOL -cholesterol; CREA -creatinine; ECL -electrochemiluminescence immunoassay system; GLUC -glucose; GGT -gamma glutamyl transferase;GSH -glutathione;Hcy -homocysteine; HHC -hyperhomocysteinemia; IBCT -total iron binding capacity;MET -DL-methionine; NAC -N-acetyl-L-cysteine; Na -sodium; RCRP -C-reactive protein; ROS -reactive oxygen species; TBI -total bilirubin; TGL -triglyceride; tHcy -total homocysteine; TP -total protein; URCA -uric acid

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Protective effects of L-carnitine, N-acetylcysteine and genistein in an experimental model of liver fibrosis

Cited by 52 publications

References 48 publications

The protective effect of N-acetyl cysteine against carbon tetrachloride toxicity in rats

The protective effect of N-acetyl cysteine against carbon tetrachloride toxicity in rats

N-acetylcysteine alleviates angiotensin II-mediated renal fibrosis in mouse obstructed kidneys

The Effects of Subchronic Methionine Overload Administered Alone or Simultaneously with L-cysteine or N-acetyl-L-cysteine on Body Weight, Homocysteine Levels and Biochemical Parameters in the Blood of Male Wistar Rats

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