Protective effects of isorhamnetin on N2a cell against endoplasmic reticulum stress-induced injury is mediated by PKCε

Qiu, Lingyu; Ma, Yurong; Luo, Yan; Cao, Zigang; Lu, Huiqiang

doi:10.1016/j.biopha.2017.06.062

Cited by 13 publications

(7 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Subsequently, the changes of ER membrane sensors-related proteins ATF6, IRE1, XBP1 and GRP78 showed that isorhamnetin relieved ZEA-induced ERS. Consistently, previous study confirmed that isorhamnetin could inhibit ERS-induced apoptosis in N2a cells ( Qiu et al., 2017 ). ERS can stimulate intracellular calcium overload ( Qiu et al., 2017 ).…”

Section: Discussionsupporting

confidence: 89%

“…Consistently, previous study confirmed that isorhamnetin could inhibit ERS-induced apoptosis in N2a cells ( Qiu et al., 2017 ). ERS can stimulate intracellular calcium overload ( Qiu et al., 2017 ). Conversely, the published data have shown that isorhamnetin inhibited Ca 2+ overload in N2a cells ( Qiu et al., 2017 ).…”

Section: Discussionsupporting

confidence: 89%

“…ERS can stimulate intracellular calcium overload ( Qiu et al., 2017 ). Conversely, the published data have shown that isorhamnetin inhibited Ca 2+ overload in N2a cells ( Qiu et al., 2017 ). In addition, pretreatment with isorhamnetin could directly decrease the level of Ca 2+ in H 2 O 2 -treated PC12 cells (Hwang et al., 2009).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Isorhamnetin protects zearalenone-induced damage via the PI3K/Akt signaling pathway in porcine ovarian granulosa cells

Chen²,

Zhang³

et al. 2022

Animal Nutrition

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 89%

See 1 more Smart Citation

Isorhamnetin protects zearalenone-induced damage via the PI3K/Akt signaling pathway in porcine ovarian granulosa cells

Chen²,

Zhang³

et al. 2022

Animal Nutrition

View full text Add to dashboard Cite

“…It also stimulated Akt activation and modulated the p38 and JNK pathways, emphasizing its role as a signaling molecule [301]. Furthermore, isorhamnetin alleviated endoplasmic reticulum stress-induced apoptosis in N2a cells by inhibiting cytosolic Ca 2+ overload, ROS production and apoptotic events, at least in part, by promoting phosphorylation of the protein kinase PKCε [302], whereas in HT22 hippocampal neurons exposed to high glucose-aggravated oxygen-glucose deprivation and reoxygenation, it attenuated apoptosis, the inflammatory response and OS via the Akt/Sirt1/Nrf2/HO-1 signaling pathway [303]. It also promoted functional recovery after spinal cord injury in rats by activating the Nrf2/HO-1 pathway, and by suppressing microglial activation and production of inflammatory cytokines [304].…”

Section: Isorhamnetinmentioning

confidence: 90%

Anti-Oxidative, Anti-Inflammatory and Anti-Apoptotic Effects of Flavonols: Targeting Nrf2, NF-κB and p53 Pathways in Neurodegeneration

et al. 2021

View full text Add to dashboard Cite

Neurodegenerative diseases are one of the leading causes of disability and death worldwide. Intracellular transduction pathways that end in the activation of specific transcription factors are highly implicated in the onset and progression of pathological changes related to neurodegeneration, of which those related to oxidative stress (OS) and neuroinflammation are particularly important. Here, we provide a brief overview of the key concepts related to OS- and neuroinflammation-mediated neuropathological changes in neurodegeneration, together with the role of transcription factors nuclear factor erythroid 2-related factor 2 (Nrf2) and nuclear factor-κB (NF-κB). This review is focused on the transcription factor p53 that coordinates the cellular response to diverse genotoxic stimuli, determining neuronal death or survival. As current pharmacological options in the treatment of neurodegenerative disease are only symptomatic, many research efforts are aimed at uncovering efficient disease-modifying agents. Natural polyphenolic compounds demonstrate powerful anti-oxidative, anti-inflammatory and anti-apoptotic effects, partially acting as modulators of signaling pathways. Herein, we review the current understanding of the therapeutic potential and limitations of flavonols in neuroprotection, with emphasis on their anti-oxidative, anti-inflammatory and anti-apoptotic effects along the Nrf2, NF-κB and p53 pathways. A better understanding of cellular and molecular mechanisms of their action may pave the way toward new treatments.

show abstract

“…Similarly, Zheng et al reported that isorhamnetin decreased CHOP and GRP78 protein expression and inhibited pulmonary ER stress in mice [55]. Isorhamnetin also suppressed ER stress-induced apoptosis in N2a cells [56]. These results indicate that isorhamnetin alleviates ZEA-induced ER stress by increasing normal ER distribution and inhibiting ER stress-related protein expression.…”

Section: Discussionmentioning

confidence: 75%

Isorhamnetin protects porcine oocytes from zearalenone-induced reproductive toxicity through the PI3K/Akt signaling pathway

Duan

Wang

et al. 2023

J Animal Sci Biotechnol

View full text Add to dashboard Cite

Background Zearalenone (ZEA) widely exists in moldy grains, which seriously destroys the fertility of females. Isorhamnetin, a natural flavonoid, has extensive of pharmacological activities. However, the beneficial effect and the underlying molecular mechanism of isorhamnetin involvement in ZEA-induced porcine oocyte damage have not been investigated. Methods Oocytes were treated with different concentrations of ZEA (3, 5, 8 and 10 μmol/L) and isorhamnetin (5, 10, 20 and 30 μmol/L) for 44 h at 39 ℃. ZEA (5 μmol/L) and isorhamnetin (10 μmol/L) were selected for subsequent studies. Polar body exclusion rate, apoptosis rate and apoptosis related proteins, ROS levels and SOD2 protein, mitochondrial membrane potential and distribution, endoplasmic reticulum distribution and proteins expression, and PI3K, Akt and p-Akt proteins expression of oocytes were detected. In addition, the effect of PI3K antagonist (LY294002) on oocyte nuclear maturation and apoptosis were used to determine the involvement of PI3K/Akt signaling pathway. Results Our findings showed that ZEA exposure damaged oocytes and isorhamnetin therapy restored the developmental capability of porcine oocytes. Isorhamnetin promoted polar body extrusion rate to rescue ZEA-induced meiotic arrest in porcine oocytes. Isorhamnetin alleviated ZEA-induced oxidative stress by stimulating SOD2 protein expression and inhibiting ROS production. Moreover, isorhamnetin enhanced normal mitochondrial distribution and mitochondrial membrane potential to prevent mitochondrial dysfunction induced by ZEA. Changing the expression of endoplasmic reticulum stress-related marker proteins (CHOP, GRP78) and the distribution rate of normal endoplasmic reticulum showed that isorhamnetin relieved ZEA-caused endoplasmic reticulum stress. Mechanistically, isorhamnetin decreased Bax/Bcl-2 protein expression and inhibited ZEA-induced apoptosis through PI3K/Akt signaling pathway. Conclusions Collectively, these results suggest that isorhamnetin protects oocytes from ZEA-caused damage through PI3K/Akt signaling pathway, which enhances meiotic maturation and mitochondrial function, and inhibits early apoptosis, oxidative stress and endoplasmic reticulum stress in porcine oocytes. Our study provides a new strategy for solving the reproductive toxicity induced by ZEA and treating woman infertility. Graphical Abstract A possible mechanism by which isorhamnetin protected porcine oocytes from ZEA-induced damage. Isorhamnetin inhibited meiosis arrest and apoptosis of porcine oocytes induced by ZEA through the PI3K/Akt signaling pathway. Moreover, isorhamnetin repaired ZEA-induced oocyte damage by alleviating oxidative stress, mitochondrial dysfunction and ER stress.

show abstract

Protective effects of isorhamnetin on N2a cell against endoplasmic reticulum stress-induced injury is mediated by PKCε

Cited by 13 publications

References 49 publications

Isorhamnetin protects zearalenone-induced damage via the PI3K/Akt signaling pathway in porcine ovarian granulosa cells

Isorhamnetin protects zearalenone-induced damage via the PI3K/Akt signaling pathway in porcine ovarian granulosa cells

Anti-Oxidative, Anti-Inflammatory and Anti-Apoptotic Effects of Flavonols: Targeting Nrf2, NF-κB and p53 Pathways in Neurodegeneration

Isorhamnetin protects porcine oocytes from zearalenone-induced reproductive toxicity through the PI3K/Akt signaling pathway

Contact Info

Product

Resources

About