Protective effects of interleukin-6 in lipopolysaccharide (LPS)-induced experimental endotoxemia are linked to alteration in hepatic anti-oxidant enzymes and endogenous cytokines

Nandi, Debolina; Mishra, Manoj Kumar; Basu, Anirban; Bishayi, Biswadev

doi:10.1016/j.imbio.2009.08.003

Cited by 45 publications

(35 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Equal weights of lung samples of control (infected-only) mice and of mice infected and treated with antibiotics either alone or in combination were homogenized in sterile PBS (1 ml). The supernatants were collected and analyzed for NO production by a modified Griess method, as described earlier (46). Briefly, nitrate was converted to nitrites with ␤-NADP (NADPH; 1.25 mg/ml) and nitrate reductase, followed by the addition of the Griess reagent.…”

Section: Methodsmentioning

confidence: 99%

Levofloxacin-Ceftriaxone Combination Attenuates Lung Inflammation in a Mouse Model of Bacteremic Pneumonia Caused by Multidrug-Resistant Streptococcus pneumoniae via Inhibition of Cytolytic Activities of Pneumolysin and Autolysin

Majhi

Adhikary

Bhattacharyya

et al. 2014

Antimicrob Agents Chemother

Self Cite

View full text Add to dashboard Cite

In this study, our objective was to determine whether a synergistic antimicrobial combination in vitro would be beneficial in the downregulation of pneumococcal virulence genes and whether the associated inflammation of the lung tissue induced by multidrug-resistant Streptococcus pneumoniae infection in vivo needs to be elucidated in order to consider this mode of therapy in case of severe pneumococcal infection. We investigated in vivo changes in the expression of these virulence determinants using an efficacious combination determined in previous studies. BALB/c mice were infected with 10 6 CFU of bacteria. Intravenous levofloxacin at 150 mg/kg and/or ceftriaxone at 50 mg/kg were initiated 18 h postinfection; the animals were sacrificed 0 to 24 h after the initiation of treatment. The levels of cytokines, chemokines, and C-reactive protein (CRP) in the serum and lungs, along with the levels of myeloperoxidase and nitric oxide the inflammatory cell count in bronchoalveolar lavage fluid (BALF), changes in pneumolysin and autolysin gene expression and COX-2 and inducible nitric oxide synthase (iNOS) protein expression in the lungs were estimated. Combination therapy downregulated inflammation and promoted bacterial clearance. Pneumolysin and autolysin expression was downregulated, with a concomitant decrease in the expression of COX-2 and iNOS in lung tissue. Thus, the combination of levofloxacin and ceftriaxone can be considered for therapeutic use even in cases of pneumonia caused by drug-resistant isolates.

show abstract

Section: Methodsmentioning

confidence: 99%

Levofloxacin-Ceftriaxone Combination Attenuates Lung Inflammation in a Mouse Model of Bacteremic Pneumonia Caused by Multidrug-Resistant Streptococcus pneumoniae via Inhibition of Cytolytic Activities of Pneumolysin and Autolysin

Majhi

Adhikary

Bhattacharyya

et al. 2014

Antimicrob Agents Chemother

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although mice are believed to be less susceptible to LPS than humans, high LPS doses (10-40 mg/kg) can produce symptoms characteristic of toxic shock that may lead to death. On the other hand, when a single, relatively low dose of bacterial endotoxin is administered to mice, death can be avoided while preserving the characteristic immune response (Zhang et al 2008, Nandi et al 2010. For example, CBA mice 10-day survival after 100 and 200 μg LPS i.v.…”

Section: Introductionmentioning

confidence: 99%

The effect of silver nanoparticles on splenocyte activity and selected cytokine levels in the mouse serum at early stage of experimental endotoxemia

Małaczewska¹

2011

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

The objective of this study was to determine the effect of a nonionic silver nanocolloid administered orally for 7 or 14 days at three concentration levels (25 ppm, 2.5 ppm, and 0.25 ppm) on the phagocytic activity and mitogenic response of splenocytes and selected cytokine serum levels (IL-1β, IL-6, IL-10, IL-12 p70, TNF-α) in NMRI mice at the early stage of experimental endotoxemia induced with single 30 μg/mouse dose of bacterial LPS.Regardless of the period of administration, silver nanoparticles enhanced the production of proinflammatory cytokines and anti-inflammatory cytokine IL-10, and they inhibited IL-12 p70 levels in response to LPS challenge. The studied nanoparticles' effect on splenocyte activity was determined by the period of administration. After 7 days of use, silver nanoparticles enhanced the phagocytic activity, and doses of 2.5 ppm stimulated the mitogenic response of splenocytes. After 14 days of administration, silver nanoparticles lowered the phagocytic activity regardless of the dose applied. Although the results obtained are ambiguous, they suggest that silver nanoparticles administered via the alimentary tract are more likely to increase an inflammatory response of an organism than offer protection after LPS challenge.

show abstract

“…Hence, TNF-α and IL-6 play a central role in the pathologic process of SIRS initiation and organ injury. [27,28] In this study, we found that the protective effects of Gln on the liver and other organ injury can be explained: the upregulation of HSP70 repaired injured proteins, promoted the degradation following irreparable injury, and attenuated the release of TNF-α and IL-6 in sepsis.…”

Section: Discussionmentioning

confidence: 72%

Protective effect of glutamine in critical patients with acute liver injury

Ni¹,

Zheng²,

Qin³

2011

World Journal of Emergency Medicine

View full text Add to dashboard Cite

Protective effects of interleukin-6 in lipopolysaccharide (LPS)-induced experimental endotoxemia are linked to alteration in hepatic anti-oxidant enzymes and endogenous cytokines

Cited by 45 publications

References 58 publications

Levofloxacin-Ceftriaxone Combination Attenuates Lung Inflammation in a Mouse Model of Bacteremic Pneumonia Caused by Multidrug-Resistant Streptococcus pneumoniae via Inhibition of Cytolytic Activities of Pneumolysin and Autolysin

Levofloxacin-Ceftriaxone Combination Attenuates Lung Inflammation in a Mouse Model of Bacteremic Pneumonia Caused by Multidrug-Resistant Streptococcus pneumoniae via Inhibition of Cytolytic Activities of Pneumolysin and Autolysin

The effect of silver nanoparticles on splenocyte activity and selected cytokine levels in the mouse serum at early stage of experimental endotoxemia

Protective effect of glutamine in critical patients with acute liver injury

Contact Info

Product

Resources

About