Protective Effects of Hydrogen-Rich Saline on Rats with Smoke Inhalation Injury

Abstract

Objective. To explore the protective effects of hydrogen-rich saline on rats with smoke inhalation injury. Methods. 36 healthy male Sprague-Dawley rats were randomly divided into 3 groups (n = 12 per group): sham group (S), inhalation injury plus normal saline treatment group (I+NS), and inhalation injury plus hydrogen-rich saline treatment group (I+HS). 30 min after injury, normal saline and hydrogen-rich saline were injected intraperitoneally (5 mL/kg) in I+NS group and I+HS group, respectively. All rats were euthanized and blood and organ specimens were collected for determination 24 h after inhalation injury. Results. Tumor necrosis factor-alpha (TNF-α) levels, malondialdehyde (MDA) concentrations, nuclear factor kappa B (NF-κB) p65 expression, and apoptosis index (AI) in I+HS group were significantly decreased (P < 0.05), while superoxide dismutase (SOD) activities were increased compared with those in I+NS group; and a marked improvement in alveolar structure was also found after hydrogen-rich saline treatment. Conclusions. Hydrogen-rich saline treatment exerts protective effects in acute lung injury induced by inhalation injury, at least in part through the activation of anti-inflammatory and antioxidant pathways and inhibition of apoptosis.

Hydrogen: A Potential New Adjuvant Therapy for COVID-19 Patients

“…Cytokines released excessively can cause acute lung injury in patients. An increase in TNF-α levels will lead to the activation of inflammatory cytokines such as IL-1, IL-6, and IL-8 ( Chen et al, 2015 ). At the same time, high mobility group box1 (HMGB1) ( Ma et al, 2015 ), CCL2 ( Hillman et al, 2007 ), and Egr-1 ( Hoetzel et al, 2008 ) all affect the release of inflammatory factors.…”

Animal Models Reflecting Chronic Obstructive Pulmonary Disease and Related Respiratory Disorders: Translating Pre-Clinical Data into Clinical Relevance

“…Mucociliary clearance is markedly increased in rats with average particulate matter cleared from the lung within 8 h, although this process can take in excess of 24 h in humans [240]. Other disadvantages to using rat models include the fact that they are obligate nose breathers [235], possess no goblet cells [23], and possess significantly fewer mucosal glands than larger animals, rendering chronic bronchitis modeling difficult [242]. Guinea Pigs Guinea pigs have been studied extensively in respiratory experimentation, particularly in the field of tuberculosis [243][244][245].…”

Molecular hydrogen: a preventive and therapeutic medical gas for various diseases

“…Studies have also shown that H 2 improves lung injuries induced by many other factors, such as hyperoxia [ 163 , 164 ], lipopolysaccharides [ 14 , 17 ], smoke inhalation [ 165 ], paraquat[ 166 ], monocrotaline [ 167 ], and extensive burns [ 168 ]. A 2013 study showed that HS pretreatment ameliorated cigarette smoking-induced airway mucus production and airway epithelium damage in rats [ 169 ].…”