Protective effects of hesperidin against oxidative stress of tert-butyl hydroperoxide in human hepatocytes

Chen, Mingcang; Gu, Heng; Ye, Yiyi; Lin, Bing; Sun, Lijuan; Deng, Weiping; Zhang, Jingzhe; Liu, Jianwen

doi:10.1016/j.fct.2010.07.037

Cited by 102 publications

(62 citation statements)

References 44 publications

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“…The antioxidant effects of hesperidin have been demonstrated in various models [2,3,4,5,6]. Therefore, it is not surprising that this flavone by itself decreased liver lipid peroxidation to values below the control (fig.…”

Section: Discussionmentioning

confidence: 99%

“…The anti-inflammatory and antifibrotic effects of hesperidin can be explained, at least in part, by the antioxidant properties described by Chen et al [3], who assessed the capability of hesperidin to prevent tert- butyl hydroperoxide-induced cell damage through the augmentation of the cellular antioxidant defence system. They suggested that hesperidin has potential as a therapeutic agent in the treatment of oxidative stress-related hepatocyte injury and liver dysfunctions; our results support this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

“…Hesperidin possesses high potential as a therapeutic agent in the treatment of oxidative stress-related hepatocyte injury and dysfunction in acute liver damage [3]. However, there is a lack of long-term analysis to evaluate the effects of hesperidin on liver fibrosis and cirrhosis.…”

Section: Introductionmentioning

confidence: 99%

“…Hesperidin has been known since antiquity for its therapeutic properties including the treatment of retinopathy caused by diabetes, decreased platelet aggregation and blood flow impairment; moreover, there is evidence that this compound has analgesic, hypolipidaemic, antihypertensive and diuretic properties [2]. Its beneficial effects have been related to the promotion of endogenous antioxidant defence systems [3]. Hesperidin also has significant anti-inflammatory activity because it modulates prostaglandin synthesis by regulating the activity of cyclooxygenase 2 (COX-2) [4].…”

Section: Introductionmentioning

confidence: 99%

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Hesperidin Prevents Liver Fibrosis in Rats by Decreasing the Expression of Nuclear Factor-κB, Transforming Growth Factor-ß and Connective Tissue Growth Factor

Pérez-Vargas¹,

Zarco²,

Shibayama

et al. 2014

Pharmacology

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Background/Aims: To evaluate the antioxidant, immunomodulatory, antinecrotic and antifibrotic effects of hesperidin on CCl₄-induced cirrhosis. Methods: Liver damage was produced by giving CCl₄ injections (0.4 g/kg, i.p., 3 times per week for 8 weeks) to rats. Hesperidin (200 mg/kg) was administered using gavage. The expression of nuclear factor-γB (NF-γB), transforming growth factor-ß (TGF-ß), connective tissue growth factor (CTGF), interleukin (IL)-10 and IL-1ß was assessed using Western blotting. Alanine aminotransferase (ALT) and γ-glutamyl transpeptidase (γ-GTP) serum activities, glycogen content, reduced/oxidised glutathione (GSH/GSSG) ratio, lipid peroxidation degree and fibrosis (using hydroxyproline content and a histopathological analysis) were measured. Results: CCl₄ increased the enzymatic activities of ALT and γ-GTP, liver lipid peroxidation, the hydroxyproline content as well as NF-γB, TGF-ß, CTGF, IL-1ß and IL-10 levels and decreased the glycogen content and GSH/GSSG ratio. Hesperidin significantly decreased the modifications produced by CCl₄, except in the case of IL-10, which was further increased by the flavone. The group receiving hesperidin alone showed decreases in lipid peroxidation, NF-γB, TGF-ß, CTGF and IL-1ß and an increase in IL-10. The results of the histopathological analysis were in agreement with the biochemical and molecular findings. Conclusions: This study demonstrates that hesperidin prevents experimental necrosis and fibrosis. The action mechanism of hesperidin is associated with its ability to reduce oxidative stress and modulate proinflammatory and profibrotic signals. These results support earlier findings demonstrating the beneficial effect of hesperidin against liver damage.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hesperidin Prevents Liver Fibrosis in Rats by Decreasing the Expression of Nuclear Factor-κB, Transforming Growth Factor-ß and Connective Tissue Growth Factor

Pérez-Vargas¹,

Zarco²,

Shibayama

et al. 2014

Pharmacology

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“…Antes de la extracción de ARN, las células HepG2 se trataron durante 24 horas con los cuatro extractos etanólicos, y como controles positivos se emplearon las sustancias genotóxicas óxido de 4-notroquinolina (4NQO), benzo(a)pireno (B(a)P), y etopósido, y el inductor de estrés oxidativo terbutil hidroperóxido (t-BuOOH) (9,(19)(20)(21)(22)(23)(24).…”

Section: Extracción De Arn Y Reacción En Cadena Cuantitativa De La Pounclassified

Mutagenicity, genotoxicity and gene expression of Rad51C, Xiap, P53 and Nrf2 induced by antimalarial extracts of plants collected from the middle Vaupés region, Colombia

et al. 2017

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Introducción. Dada la resistencia de Plasmodium a los medicamentos antipalúdicos, es necesario encontrar nuevas alternativas terapéuticas para su tratamiento y control. Con base en el saber indígena colombiano, se recopilaron extractos de plantas del Vaupés medio con potencial efecto antipalúdico.Objetivo. Evaluar el efecto mutagénico y genotóxico, y la expresión de los genes Rad51C, Xiap, P53 y Nrf2, inducidos por cuatro extractos etanólicos con actividad anti-Plasmodium (R001, T002, T015 y T028).Materiales y métodos. Se evaluó el potencial mutagénico de cuatro extractos etanólicos con efecto antiplasmódico utilizando el test de Ames y el efecto genotóxico, con un ensayo del cometa; asimismo, se analizó la expresión de los genes Rad51C, Xiap, P53 y Nrf2 en células HepG2.Resultados. Los extractos no fueron mutágenos en la cepa TA98 de Salmonella typhimurium en presencia y ausencia de actividad metabólica de la fracción S9. En la cepa TA100, los extractos R001, T015 y T028 se comportaron como mutágenos débiles en presencia de S9, con índices mutagénicos de 1,58; 1,38; 1,53 y 1,61, respectivamente; T015 tuvo el mismo comportamiento en ausencia de S9, con un índice mutagénico de 1,36. En el ensayo del cometa, todos los extractos provocaron daño de categorías 1 o 2, con colas de cometas entre 36,7 y 51,48 μm de longitud; sin embargo, el índice de daño genético sugirió que los tratamientos afectaron la mayoría de las células. En los genes en estudio, los extractos R001 y T028 indujeron una sobreexpresión de 1,84 a 3,99 frente a las células sin tratar de los genes Xiap y P53.Conclusiones. Los resultados evidenciaron que el extracto T002 fue el más seguro, ya que presentó actividad anti-Plasmodium, no fue citotóxico en las células HepG2, no fue mutágeno, causó daño de categoría 1 en el ADN y no modificó la expresión de los genes evaluados.

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Potential Value‐Added Co‐products from Citrus Fruit Processing

Manthey

Bergeron²,

Carrier

et al. 2012

Biorefinery Co‐Products

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Protective effects of hesperidin against oxidative stress of tert-butyl hydroperoxide in human hepatocytes

Cited by 102 publications

References 44 publications

Hesperidin Prevents Liver Fibrosis in Rats by Decreasing the Expression of Nuclear Factor-κB, Transforming Growth Factor-ß and Connective Tissue Growth Factor

Hesperidin Prevents Liver Fibrosis in Rats by Decreasing the Expression of Nuclear Factor-κB, Transforming Growth Factor-ß and Connective Tissue Growth Factor

Mutagenicity, genotoxicity and gene expression of Rad51C, Xiap, P53 and Nrf2 induced by antimalarial extracts of plants collected from the middle Vaupés region, Colombia

Potential Value‐Added Co‐products from Citrus Fruit Processing

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Protective effects of hesperidin against oxidative stress of tert-butyl hydroperoxide in human hepatocytes

Cited by 102 publications

References 44 publications

Hesperidin Prevents Liver Fibrosis in Rats by Decreasing the Expression of Nuclear Factor-&#954;B, Transforming Growth Factor-ß and Connective Tissue Growth Factor

Hesperidin Prevents Liver Fibrosis in Rats by Decreasing the Expression of Nuclear Factor-&#954;B, Transforming Growth Factor-ß and Connective Tissue Growth Factor

Mutagenicity, genotoxicity and gene expression of Rad51C, Xiap, P53 and Nrf2 induced by antimalarial extracts of plants collected from the middle Vaupés region, Colombia

Potential Value‐Added Co‐products from Citrus Fruit Processing

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Product

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Hesperidin Prevents Liver Fibrosis in Rats by Decreasing the Expression of Nuclear Factor-κB, Transforming Growth Factor-ß and Connective Tissue Growth Factor

Hesperidin Prevents Liver Fibrosis in Rats by Decreasing the Expression of Nuclear Factor-κB, Transforming Growth Factor-ß and Connective Tissue Growth Factor