Protective Effects of Glucosamine on Oxidative-Stress and Ischemia/Reperfusion-Induced Retinal Injury

Chen, Ying-Jen; Huang, Yuahn-Sieh; Chen, Jiann‐Torng; Chen, Yi-Hao; Tai, Ming‐Cheng; Chen, Ching‐Long; Liang, Chang-Min

doi:10.1167/iovs.14-15726

Cited by 49 publications

(42 citation statements)

References 53 publications

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“…These results were identity and confirming the findings of some investigators [9], who reported that glucosamine has accelerated the process of wound healing, re-epithelization and the tissue regeneration rate. Preservation of the histological structures with glucosamine is explained by some investigators [8,10,29] stated that glucosamine possesses an immune-modulatory influence by decreasing inflammation, neutrophil recruitment through the down regulation of inflammatory factors as TNF-α, IL-1, and IL-6. Moreover, GlcN increases the antioxidant activity owing to its constituents such as flavonoids, phenols, Tannis (Phenolic compounds) and Triterpenoids.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, GlcN increases the antioxidant activity owing to its constituents such as flavonoids, phenols, Tannis (Phenolic compounds) and Triterpenoids. Also, it prevents DNA damage and reduces apoptosis and accelerates the cellular growth and proliferation in vitro [10,[29][30][31].…”

Section: Discussionmentioning

confidence: 99%

“…Rats injected once intraperitoneally with 5-FU (150mg/kg body weight) with PBS as the vehicle of 5-FU [10].…”

Section: Group II (Mucositis)mentioning

confidence: 99%

“…Glucosamine possess extreme biological effects as membrane stabilization and hepatic preservation. It also augments the synthesis of hyaluronic acid and is applied for the curing of osteoarthritis and skin injuries [9,10].…”

Section: Introductionmentioning

confidence: 99%

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Histological and immunohistochemical study of the potential healing effects of glucosamine and platelets rich plasma on experimentally induced oral mucositis in adult male rats

Mansy¹,

El-Azab²,

El-Mahalaway³

2017

J Histol Histopathol

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Background: Oral mucositis (OM) is mucosal barrier injury. It is commonly caused by 5 Flurouracil (5-FU). Glucosamine (GlcN) has many beneficial therapeutic effects. Platelets rich plasma (PRP) are a promising line for curing ofvarious clinical injuries. Objective: The aim of the study was to evaluate the effect of glucosamine and PRP on experimentally induced oral mucositis in adult male rats. Materials and methods: 40 adult male rats divided evenly into four groups. Group I (Control). Group II (Mucositis): rats injected one time intraperitoneally with 5-FU(150 mg/kg body weight).Group (III) (Mucositis treated with glucosamine): rats injected intraperitoneally with glucosamine (1000mg/kg body weight) following induction of oral mucositis for 3weeks. Group IV (Mucositis treated with PRP) rats injected with PRP(1ml/rat/day) locally into buccal mucosa following induction of oral mucositis for 3weeks. Buccal mucosa samples prepared and examined by using histological and immunohistochemical techniques. Results: Group II showed apparent diminish in the thickness of the epithelium with separation of keratin, hemorrhage and perinuclear vacuolization. There were areas of degeneration, intense subepithelial mononuclear infiltration and cell apoptosis (highly expressed caspase-3). A significant reduction of Periodic acid Schiff (PAS) positive reaction and anti-proliferating cell nuclear antigen (PCNA) positive nuclei (P<0.01). Group III&IV showed improvement of the histological and immunohistochemical changes described before. Conclusion: Glucosamine and PRP provide a promising treatment for oral mucositis accompanying with chemotherapy. PRP remedy is simple and the most efficient mean as it exhibits more rapid epithelial differentiation and wound healing.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Rats injected once intraperitoneally with 5-FU (150mg/kg body weight) with PBS as the vehicle of 5-FU [10].…”

Section: Group II (Mucositis)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Histological and immunohistochemical study of the potential healing effects of glucosamine and platelets rich plasma on experimentally induced oral mucositis in adult male rats

Mansy¹,

El-Azab²,

El-Mahalaway³

2017

J Histol Histopathol

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“…Moreover, Jensen et al suggested that the protective effects of remote ischemic (RIPC) and ischemic preconditioning (IPC) are due to increased circulating GlcNAc levels and O-GlcNAcylation, as well [10]. The positive effects of the HP do not appear to be limited to the heart since GlcNAc administration before and especially after ischemia of the eye has been shown to mediate retinal cell survival [11]. …”

Section: Introductionmentioning

confidence: 99%

Oral Supplementation of Glucosamine Fails to Alleviate Acute Kidney Injury in Renal Ischemia-Reperfusion Damage

et al. 2016

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Acute kidney injury is a leading contributor to morbidity and mortality in the ageing population. Proteotoxic stress response pathways have been suggested to contribute to the development of acute renal injury. Recent evidence suggests that increased synthesis of N-glycan precursors in the hexosamine pathway as well as feeding of animals with aminosugars produced in the hexosamine pathway may increase stress resistance through reducing proteotoxic stress and alleviate pathology in model organisms. As feeding of the hexosamine pathway metabolite glucosamine to aged mice increased their life expectancy we tested whether supplementation of this aminosugar may also protect mice from acute kidney injury after renal ischemia and reperfusion. Animals were fed for 4 weeks ad libitum with standard chow or standard chow supplemented with 0.5% N-acetylglucosamine. Preconditioning with caloric restriction for four weeks prior to surgery served as a positive control for protective dietary effects. Whereas caloric restriction demonstrated the known protective effect both on renal function as well as survival in the treated animals, glucosamine supplementation failed to promote any protection from ischemia-reperfusion injury. These data show that although hexosamine pathway metabolites have a proven role in enhancing protein quality control and survival in model organisms oral glucosamine supplementation at moderate doses that would be amenable to humans does not promote protection from ischemia-reperfusion injury of the kidney.

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Retinal Microenvironment‐Protected Rhein‐GFFYE Nanofibers Attenuate Retinal Ischemia‐Reperfusion Injury via Inhibiting Oxidative Stress and Regulating Microglial/Macrophage M1/M2 Polarization

Zhang,

Peng,

et al. 2023

Advanced Science

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Retinal ischemia is involved in the occurrence and development of various eye diseases, including glaucoma, diabetic retinopathy, and central retinal artery occlusion. To the best of our knowledge, few studies have reported self‐assembling peptide natural products for the suppression of ocular inflammation and oxidative stress. Herein, a self‐assembling peptide GFFYE is designed and synthesized, which can transform the non‐hydrophilicity of rhein into an amphiphilic sustained‐release therapeutic agent, and rhein‐based therapeutic nanofibers (abbreviated as Rh‐GFFYE) are constructed for the treatment of retinal ischemia‐reperfusion (RIR) injury. Rh‐GFFYE significantly ameliorates oxidative stress and inflammation in an in vitro oxygen‐glucose deprivation (OGD) model of retinal ischemia and a rat model of RIR injury. Rh‐GFFYE also significantly enhances retinal electrophysiological recovery and exhibits good biocompatibility. Importantly, Rh‐GFFYE also promotes the transition of M1‐type macrophages to the M2 type, ultimately altering the pro‐inflammatory microenvironment. Further investigation of the treatment mechanism indicates that Rh‐GFFYE activates the PI3K/AKT/mTOR signaling pathway to reduce oxidative stress and inhibits the NF‐κB and STAT3 signaling pathways to affect inflammation and macrophage polarization. In conclusion, the rhein‐loaded nanoplatform alleviates RIR injury by modulating the retinal microenvironment. The findings are expected to promote the clinical application of hydrophobic natural products in RIR injury‐associated eye diseases.

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Protective Effects of Glucosamine on Oxidative-Stress and Ischemia/Reperfusion-Induced Retinal Injury

Abstract: Glucosamine treatment provides multiple levels of retinal protection, including antiapoptotic, anti-inflammatory, and antioxidative benefits. More research on the role of GlcN as a potential agent for the prevention and treatment of glaucoma is warranted.

Cited by 49 publications

References 53 publications

Histological and immunohistochemical study of the potential healing effects of glucosamine and platelets rich plasma on experimentally induced oral mucositis in adult male rats

Histological and immunohistochemical study of the potential healing effects of glucosamine and platelets rich plasma on experimentally induced oral mucositis in adult male rats

Oral Supplementation of Glucosamine Fails to Alleviate Acute Kidney Injury in Renal Ischemia-Reperfusion Damage

Retinal Microenvironment‐Protected Rhein‐GFFYE Nanofibers Attenuate Retinal Ischemia‐Reperfusion Injury via Inhibiting Oxidative Stress and Regulating Microglial/Macrophage M1/M2 Polarization

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