Protective effects of garlic sulfur compounds against DNA damage induced by direct- and indirect-acting genotoxic agents in HepG2 cells

Belloir, Christine; Singh, Varsha; Daurat, C.; Siess, Marie‐Hélène; Bon, Anne‐Marie Le

doi:10.1016/j.fct.2005.11.005

Cited by 80 publications

(49 citation statements)

References 49 publications

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“…The cytotoxic effects of DAS have been reported to occur at concentrations ranging from 150 to 500 g/ml, and several other studies have shown that lower concentrations (5 to 100 M) of the compounds have no cytotoxic effect (3,10,25,31). The antimicrobial effects observed in our study were found at a concentration of 0.01 g/ml.…”

Section: Discussionsupporting

confidence: 70%

In Vitro Efficacy of Diallyl Sulfides against the Periodontopathogen Aggregatibacter actinomycetemcomitans

Velliyagounder

Ganeshnarayan

Velusamy

et al. 2012

Antimicrob Agents Chemother

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The in vitro antibacterial effects of diallyl sulfide (DAS) against the Gram-negative periodontopathogen Aggregatibacter actinomycetemcomitans, the key etiologic agent of the severe form of localized aggressive periodontitis and other nonoral infections, were studied. A. actinomycetemcomitans was treated with garlic extract, allicin, or DAS, and the anti-A. actinomycetemcomitans effects of the treatment were evaluated. Garlic extract, allicin, and DAS significantly inhibited the growth of A. actinomycetemcomitans (greater than 3 log; P < 0.01) compared to control cells. Heat inactivation of the garlic extracts significantly reduced the protein concentration; however, the antimicrobial effect was retained. Purified proteins from garlic extract did not exhibit antimicrobial activity. Allicin lost all its antimicrobial effect when it was subjected to heat treatment, whereas DAS demonstrated an antimicrobial effect similar to that of the garlic extract, suggesting that the antimicrobial activity of garlic extract is mainly due to DAS. An A. actinomycetemcomitans biofilm-killing assay performed with DAS showed a significant reduction in biofilm cell numbers, as evidenced by both confocal microscopy and culture. Scanning electron microscopy (SEM) analysis of DAS-treated A. actinomycetemcomitans biofilms showed alterations of colony architecture indicating severe stress. Flow cytometry analysis of OBA9 cells did not demonstrate apoptosis or cell cycle arrest at therapeutic concentrations of DAS (0.01 and 0.1 g/ml). DAStreated A. actinomycetemcomitans cells demonstrated complete inhibition of glutathione (GSH) S-transferase (GST) activity. However, OBA9 cells, when exposed to DAS at similar concentrations, showed no significant differences in GST activity, suggesting that DAS-induced GST inhibition might be involved in A. actinomycetemcomitans cell death. These findings demonstrate that DAS exhibits significant antibacterial activity against A. actinomycetemcomitans and that this property might be utilized for exploring its therapeutic potential in treatment of A. actinomycetemcomitans-associated oral and nonoral infections.

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Section: Discussionsupporting

confidence: 70%

In Vitro Efficacy of Diallyl Sulfides against the Periodontopathogen Aggregatibacter actinomycetemcomitans

Velliyagounder

Ganeshnarayan

Velusamy

et al. 2012

Antimicrob Agents Chemother

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“…It was reported that anti-genotoxic compounds consumed within the diet could decrease the increased number of these cases 1 . Plant extracts rich in polyphenols have antimutagenic potentials and DNA-protecting effects 14 .…”

Section: Discussionmentioning

confidence: 99%

“…Garlic has antimutagenic, anticarcinogenic, antithrombotic, antineoplastic and antioxidant properties 2,4 . Experimental and epidemiological studies have shown that there is a correlation between garlic consumption and lower rate of cancer incidences [1][2][3] . It is reported that organosulfur compounds found in garlic confer its antitumor action 4 .…”

Section: Introductionmentioning

confidence: 99%

“…Recently, there has been a great deal of interest about the anti-mutagenic and anti-carcinogenic potential of compounds derived from plants and natural food stuff 2 . Garlic which is produced and consumed worldwide has drawn attention for its protective effects against a number of disorders 1,3 . Epidemiological studies showed that garlic contains many biologically and pharmacologically active compounds 3 .…”

Section: Introductionmentioning

confidence: 99%

“…It is known that diet rich in vegetables and fruits has the potential to lower the risk of cancer 1 . Recently, there has been a great deal of interest about the anti-mutagenic and anti-carcinogenic potential of compounds derived from plants and natural food stuff 2 .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In Vitro Antimutagenicity of Allium Tuncelianum Ethanol Extract Against Induction of Chromosome Aberration by Mutagenic Agent Mitomycine C

Özkan¹,

Gül²,

Kart³

et al. 2013

Kafkas Univ Vet Fak Derg

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SummaryAllium Tuncelianum is a garlic species which is locally produced in Tunceli, Turkey. Garlic contains several biologically active compounds known as organosulfur compounds which are reported to have anticarcinogenic and antimutagenic effects. Present study examined the in vitro antigenotoxic activity of ethanolic extracts of A. Tuncelianum in human lymphocyte cells by chromosome aberration test. Samples of A. Tuncelianum were extracted by ethanol using soxhlet extractor. The extract was tested on human lymphocyte cell culture in vitro chromosome aberration test. The effect of ethanol extract of A. Tuncelianum at concentrations of 0.10, 0.15, 0.20, 0.25 µl/mL were screened for chromatid and chromosome breaks, chromosome exchange as well as chromatid union and polyploid cells against negative acetone and positive control Mitomycine C. A significant decrease in the frequency of chromosomal aberration was observed for all treatments with A. Tuncelianum ethanol extract at 24 h. In conclusion, ethanol extract of A. tuncelianum significantly reduced the chromosomal aberration rate as compared with the culture treated with MMC. The results could also indicate that A. Tuncelianum could be protective against mutagenic and carcinogenic compounds when consumed through the diet.

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Medicinal Chemistry and Therapeutic Applications of the GasotransmittersNO,CO, andH₂Sand their Prodrugs

Szabó

2010

Burger's Medicinal Chemistry and Drug Discovery

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Nitric oxide ( NO ), carbon monoxide ( CO ), and hydrogen sulfide ( H 2 S ) are the main endogenous gasotransmitters. These gases are produced by the body by enzymatic reactions and serve physiological regulatory purposes including vasodilatation and anti‐inflammatory effects. Over the past decades, multiple approaches have been identified for the therapeutic exploitation of these three gasotransmitters, based on inhalation of the gases and/or the parenteral or enteral administration of various formulations of these molecules or their prodrugs. Here we overview the medicinal chemistry and the therapeutic applications of NO, CO, and H 2 S and their prodrugs. Inhaled NO is used clinically to selectively dilate the pulmonary vasculature in the therapy of the primary pulmonary hypertension of the newborn. Organic nitrates such as glyceryl trinitrate or nitroglycerin are used in the therapy of acute and chronic angina. Sodium nitroprusside is used to counteract acute hypertensive crisis. Another class of clinical‐stage NO donor drugs is the sydnonimines (molsidomine, linsidomine). Additional classes of NO donors include diazeniumdiolates (NONOates) and S ‐nitrosothiols, with beneficial effects in various preclinical models of disease. With respect to CO, the main therapeutic approaches are CO inhalation (currently in clinical trials for the experimental therapy of delayed graft function associated with kidney transplantation) and carbon monoxide releasing compounds of various classes (in preclinical stage). With respect to H 2 S, a parenteral injectable formulation of the gas is currently is Phase I trials. Several classes of H 2 S donor molecules have also been identified, which are used in preclinical studies.

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Protective effects of garlic sulfur compounds against DNA damage induced by direct- and indirect-acting genotoxic agents in HepG2 cells

Cited by 80 publications

References 49 publications

In Vitro Efficacy of Diallyl Sulfides against the Periodontopathogen Aggregatibacter actinomycetemcomitans

In Vitro Efficacy of Diallyl Sulfides against the Periodontopathogen Aggregatibacter actinomycetemcomitans

In Vitro Antimutagenicity of Allium Tuncelianum Ethanol Extract Against Induction of Chromosome Aberration by Mutagenic Agent Mitomycine C

Medicinal Chemistry and Therapeutic Applications of the GasotransmittersNO,CO, andH₂Sand their Prodrugs

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Protective effects of garlic sulfur compounds against DNA damage induced by direct- and indirect-acting genotoxic agents in HepG2 cells

Cited by 80 publications

References 49 publications

In Vitro Efficacy of Diallyl Sulfides against the Periodontopathogen Aggregatibacter actinomycetemcomitans

In Vitro Efficacy of Diallyl Sulfides against the Periodontopathogen Aggregatibacter actinomycetemcomitans

In Vitro Antimutagenicity of Allium Tuncelianum Ethanol Extract Against Induction of Chromosome Aberration by Mutagenic Agent Mitomycine C

Medicinal Chemistry and Therapeutic Applications of the GasotransmittersNO,CO, andH2Sand their Prodrugs

Contact Info

Product

Resources

About

Medicinal Chemistry and Therapeutic Applications of the GasotransmittersNO,CO, andH₂Sand their Prodrugs