Protective effects of gallocatechin gallate against ultraviolet B induced skin damages in hairless mice

Abstract

Epigallocatechin gallate (EGCG) has the effect to protect skin from ultraviolet B (UVB) induced damages, but it is unstable under ambient conditions, being susceptible to become brown in color. Gallocatechin gallate (GCG), an epimer counterpart of EGCG, is more stable chemically than EGCG. The potential effects of GCG against UVB-induced skin damages has not been available. The objective of this study was to investigate the protective effects of GCG against UVB-induced skin photodamages. GCG was topically applied on the skin of hairless mice at three dosage levels (LL, 12.5 mg/mL; ML 25 mg/mL; HL, 50 mg/mL), with EGCG and a commercially available baby sunscreen lotion SPF50 PA+++ as control. The mice were then irradiated by UVB (fluence rate 1.7 µmol/m2 s) for 45 min. The treatments were carried out once a day for 6 consecutive days. Skin measurements and histological studies were performed at the end of experiment. The results show that GCG treatments at ML and HL levels inhibited the increase in levels of skin oil and pigmentation induced by UVB irradiation, and improved the skin elasticity and collagen fibers. GCG at ML and HL levels inhibited the formation of melanosomes and aberrations in mitochondria of UVB-irradiated skin in hairless mice. It is concluded that GCG protected skin from UVB-induced photodamages by improving skin elasticity and collagen fibers, and inhibiting aberrations in mitochondria and formation of melanosomes.

Physicochemical Characterization and Evaluation of Gastrointestinal In Vitro Behavior of Alginate-Based Microbeads with Encapsulated Grape Pomace Extracts

“…The results show that the concentration of gallocatechin gallate is almost 6.45 times higher than that of epigallocatechin gallate, which is due to the fact that it is chemically much more stable than epigallocatechin gallate. These properties allow the use of gallocatechin gallate in the preparation of creams to protect the skin from UVB radiation [ 70 ]. Among the studied flavonols, quercetin stands out (470.69 μg/g db ), which has been attributed to its potential use in the treatment of eye diseases [ 71 ], as an antiviral agent [ 72 ], etc.…”

Protective Effect of Castanopsis sieboldii Extract against UVB-Induced Photodamage in Keratinocytes

“…We found that EGCG is a major constituent of CSL3, whose EGCG contents are much higher than in green tea ( Figure 3 B). EGCG is known to have a beneficial effect on UVB-damaged keratinocytes by preventing ROS generation [ 51 ] and suppressing various skin damages through its antioxidant capacity [ 52 ]. Our results confirmed that increased ROS generation after UV irradiation or t -BHP treatment is significantly reduced by EGCG treatment ( Figure 8 C).…”

Widely Untargeted Metabolome Profiling Provides Insight into Browning and Nutritional Quality Changes in Short-Term Stored Fresh-Cut Potato (Solanum tuberosum L.) Shreds

“…Of these, one flavonoid compound, isobavachalcone D, was completely absent in CK, while the remaining two flavonoids (gallocatechin and 8-methoxykaempferol-7-O-rhamnoside) accumulated 2.70 and 9.30 times higher in CK4H than in CK, respectively. Gallocatechin and its derivatives are reported to be brown in color under ambient conditions [54]. Similarly, three lignan and coumarin compounds (isofraxidin, esculetin and ayapin) accumulated 2.47-16.52 times more in CK4H than in CK.…”