Protective Effects of Flavonoids Against Oxidative Stress Induced by Simulated Microgravity in SH-SY5Y Cells

Qu, Lei; Chen, Hailong; Liu, Xinmin; Bi, Lei; Xiong, Jian; Mao, Zebin; Li, Yinghui

doi:10.1007/s11064-010-0205-4

Cited by 38 publications

(12 citation statements)

References 35 publications

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“…Wang et al also reported the protective effects of NAC on ROS production and cell senescence under SMG treatment (Wang et al 2009). Qu et al showed that antioxidants, isorhamnetin and luteolin, could protect neuroblastoma SH-SY5Y cells against microgravity-induced oxidative stress (Qu et al 2010). These results indicate that antioxidants such as NAC might be used in the protection of ROS stress induced by the combined effects of SMG and other factors of space environment, which may provide valuable strategy for health protection in manned space exploration.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, several lines of evidence showed that SMG increased ROS production in some cell types, such as the PC12 cells, SH-SY5Y cells, and MEF cells (Wang et al 2009; Qu et al 2010; Li et al 2015). It has been reported that SMG delayed the rejoining of double-strand breaks (DSBs) induced by IR and increased the genotoxic effects of IR (Mognato et al 2009).…”

Section: Introductionmentioning

confidence: 99%

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Simulated microgravity potentiates generation of reactive oxygen species in cells

Ran

Fan

et al. 2016

Biophys Rep

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Microgravity (MG) and space radiation are two major environmental factors of space environment. Ionizing radiation generates reactive oxygen species (ROS) which plays a key role in radiation-induced DNA damage. Interestingly, simulated microgravity (SMG) also increases ROS production in various cell types. Thus, it is important to detect whether SMG could potentiate ROS production induced by genotoxins including radiation, especially at a minimal level not sufficient to induce detectable ROS. In this study, we treated mouse embryonic stem (MES) cells with H2O2 and SMG for 24 h. The concentration of H2O2 used was within 30 μmol/L at which intracellular ROS was the same as that in untreated cells. Exposure of cells to SMG for 24 h did not induce significantly higher levels of intracellular ROS than that of control cells either. Simultaneous exposure of cells to both SMG- and H2O2-induced ROS and apoptosis in MES cells. Although incubation in medium containing 5 or 30 μmol/L H2O2 induced a small enhancement of DNA double-strand breaks (DSBs), the addition of SMG treatment dramatically increased DSB levels. Taken together, SMG can significantly potentiate the effects of H2O2 at a low concentration that induce a small or negligible change in cells on ROS, apoptosis, and DNA damage. The results were discussed in relation to the combined effects of space radiation and MG on human body in this study.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simulated microgravity potentiates generation of reactive oxygen species in cells

Ran

Fan

et al. 2016

Biophys Rep

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“…Clinorotation was performed at 30 rpm (revolutions per minute) according to previous studies (Dai et al 2000;Qu et al 2010), having 3 specially designed flasks with an internal diameter of 4 cm in each group. Glass slides were inserted into a specially designed shelf in every flask.…”

Section: Clinorotationmentioning

confidence: 99%

Two-dimensional clinorotation influences cellular morphology, cytoskeleton and secretion of MLO-Y4 osteocyte-like cells

Weng

et al. 2012

Biologia

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The aim of this study was to investigate the effects of the clinostat-simulated weightlessness on biological characteristics of MLO-Y4 osteocyte-like cells. MLO-Y4 cells were incubated for 24 h, then randomly divided into 3 groups and rotated in a clinostat as a model of simulated weightlessness for 12 h, 24 h and 48 h. The morphology, cytoskeleton, and secretion of soluble molecules of MLO-Y4 cells were observed and detected. The results show that clinostat culture affects the number of dendrites/cell, cytoskeleton distribution, and secretion of nitric oxide and prostaglandin E2 in MLO-Y4 cells. These results may provide some clue to explore the cellular mechanism of bone loss caused by weightlessness.

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“…Many lines of evidence suggest that conditions experienced during microgravity result in increased oxidative stress1011. Indo et al .…”

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confidence: 99%

Changes in mitochondrial homeostasis and redox status in astronauts following long stays in space

Indo

Majima

Terada

et al. 2016

Sci Rep

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The effects of long-term exposure to extreme space conditions on astronauts were investigated by analyzing hair samples from ten astronauts who had spent six months on the International Space Station (ISS). Two samples were collected before, during and after their stays in the ISS; hereafter, referred to as Preflight, Inflight and Postflight, respectively. The ratios of mitochondrial (mt) to nuclear (n) DNA and mtRNA to nRNA were analyzed via quantitative PCR. The combined data of Preflight, Inflight and Postflight show a significant reduction in the mtDNA/nDNA in Inflight, and significant reductions in the mtRNA/nRNA ratios in both the Inflight and Postflight samples. The mtRNA/mtDNA ratios were relatively constant, except in the Postflight samples. Using the same samples, the expression of redox and signal transduction related genes, MnSOD, CuZnSOD, Nrf2, Keap1, GPx4 and Catalase was also examined. The results of the combined data from Preflight, Inflight and Postflight show a significant decrease in the expression of all of the redox-related genes in the samples collected Postflight, with the exception of Catalase, which show no change. This decreased expression may contribute to increased oxidative stress Inflight resulting in the mitochondrial damage that is apparent Postflight.

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Protective Effects of Flavonoids Against Oxidative Stress Induced by Simulated Microgravity in SH-SY5Y Cells

Cited by 38 publications

References 35 publications

Simulated microgravity potentiates generation of reactive oxygen species in cells

Simulated microgravity potentiates generation of reactive oxygen species in cells

Two-dimensional clinorotation influences cellular morphology, cytoskeleton and secretion of MLO-Y4 osteocyte-like cells

Changes in mitochondrial homeostasis and redox status in astronauts following long stays in space

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