Protective Effects of Flavonoid Pomiferin on Heart Ischemia-Reperfusion

Abstract

Nečas J., L. Bartošíková, T. Florian, J. Klusáková, V. Suchý, E. Janoštíková, T. Bartošík, EM. B. El Naggar: Protective Effects of Flavonoid Pomiferin on Heart IschemiaReperfusion. Acta Vet. Brno 2007, 76: 363-370.The objective of the present 15-day study was to evaluate the cardioprotective potential of fl avonoid pomiferin isolated from the infructences of Maclura pomifera, Moraceae, against ischemia-reperfusion induced injury in rat hearts as a model of antioxidant-based composite therapy. Studies were performed with isolated, modifi ed Langendorff-perfused rat hearts and ischemia of heart was initiated by stopping the coronary fl ow for 30 min, followed by 60 min of reperfusion (14 ml·min -1 ). Wistar rats were divided into three groups. The treated group received pomiferin (5 mg/kg/day in 0.5% Avicel); the placebo group received only 0.5% Avicel; the intact group was left without any applications. Biochemical indicators of oxidative damage, lipid peroxidation product malondialdehyde, antioxidant enzymes (superoxide dismutase, glutathione peroxidase, total antioxidant activity in serum and myocardium has been evaluated. We also examined the effect of pomiferin on cardiac function (left ventricular end-diastolic pressure, left ventricular pressure, peak positive +dP/dt (rate of pressure development) after ischemia and reperfusion. Our results demonstrate that pomiferin attenuates the myocardial dysfunction provoked by ischemiareperfusion. This was confi rmed by the increase in both the antioxidant enzyme values and the total antioxidant activity. The cardio-protection provided by pomiferin treatment results from the suppression of oxidative stress and correlates with the improved ventricular function.

Cardioprotective actions of two bioflavonoids, quercetin and rutin, in experimental myocardial infarction in both normal and streptozotocin-induced type I diabetic rats

“…The protective capacity of quercetin against I/R injury is not limited to the myocardium but is also found in several other tissues, such as the kidney, 49,50 liver 51 and brain 52 . Considering the potential of the flavonoids as a whole, our result further supports previous studies reporting the limiting effect of flavonoids on infarct size 53–55 …”

“…Quercetin and rutin treatment significantly reduced the serum and tissue MDA levels in both normal and diabetic I/R injury. Although quercetin does not reverse oxidative stress in other organs collected from diabetic rats, 56 numerous earlier studies reveal that quercetin attenuates oxidative stress in experimental settings of I/R 51,53 . Furthermore, quercetin has a well‐proven antioxidant effect, in vitro as well as in vivo.…”

Luteolinidin Protects the Postischemic Heart through CD38 Inhibition with Preservation of NAD(P)(H)

“…Although luteolinidin protects the heart through its potent inhibition of CD38, it could also exert other beneficial effects. It has been suggested that flavonoids can be protective due to their antioxidant activity (Necas et al, 2006;Chang et al, 2007). In our experiments, the dose dependence of the protection (from 5 to 50 mM) argues for relatively high potency and low toxicity for luteolinidin.…”

Pliny’s pharmacopoeia or the Roman treat

“…6 Furthermore, significant antioxidant activity seems to attenuate myocardial dysfunction provoked by reperfusion ischaemia. 7 In specified clinical circumstances natural synergy, as is the case in urginea maritima, containing flavonoids and scillaglycoside with its inotropic, antiarrhythmic and vaso-active action, not to say neurohumoral modulation, 8 need not be deleterious at all. With a sense of understatement one could then argue for a full circle with herbal medicine in antiquity.…”