Protective effects of extracorporeal shockwave on rat chondrocytes and temporomandibular joint osteoarthritis; preclinical evaluation with in vivo 99mTc-HDP SPECT and ex vivo micro-CT

Kim, Young-Hwa; Bang, Ji-In; Son, Hye-Jin; Kim, Yemi; Kim, JiHoi; Bae, Hasuk; Han, Soo Jeong; Yoon, Hyun Sun; Kim, Bom Sahn

doi:10.1016/j.joca.2019.07.008

Cited by 26 publications

(21 citation statements)

References 36 publications

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“…Very recently, animal model studies have increasingly demonstrated the efficacy of ESWT for OA [16,17,19]. However, few studies have explored the clinical application of ESWT, probably due to a lack of mechanistic clarity and scientific evidence [48]. OA is characterized by the main features of articular cartilage degeneration and subchondral bone remodeling and cardinal symptoms of pain and restricted motion in the affected joints.…”

Section: Extracorporeal Shockwave Treatment For Osteoarthritismentioning

confidence: 99%

“…ESWT may also exert chondroprotective effects in OA models by restoring the production of IL-10 and TNF-α in chondrocytes to normal levels [55]. Kim et al [48] investigated effects of ESWT on temporomandibular joint osteoarthritis (TMJOA) in Rat OA models, and found ESWT treatment could increase cell viability significantly and decrease expression of cartilage degradation markers including MMP3 and MMP13. Changes in cytochrome c and cleaved caspase-3 levels relative to procaspase-3 were also decreased by ESWT, which showed a significant decrease in pro-inflammatory and cartilage degradation markers.…”

Section: Effect Of Eswt On Cartilagementioning

confidence: 99%

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Extracorporeal shockwave treatment in knee osteoarthritis: therapeutic effects and possible mechanism

Xie

et al. 2020

Bioscience Reports

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Osteoarthritis (OA), the most common degenerative joint disease, is characterized by the cardinal symptoms of chronic pain and restricted joint activity. The complicated pathological changes associated with OA and unclear mechanistic etiology have rendered existing non-surgical OA management options unsatisfactory. Increasing clinical and experimental evidence suggests that extracorporeal shockwave therapy (ESWT) is beneficial in OA treatment. ESWT is found to have modifying effects on cartilage and subchondral bone alterations in OA progression, as well as the clinical complaints of patients, including chronic pain and limited joint activities. However, the specific treatment strategy regarding the dosage and frequency of ESWT is still underdetermined. This review discusses the existing evidence regarding the therapeutic indications and possible mechanism of ESWT for OA treatment.

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Section: Extracorporeal Shockwave Treatment For Osteoarthritismentioning

confidence: 99%

Section: Effect Of Eswt On Cartilagementioning

confidence: 99%

Extracorporeal shockwave treatment in knee osteoarthritis: therapeutic effects and possible mechanism

Xie

et al. 2020

Bioscience Reports

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“…Recent work has identified a battery of proteins that regulate processing (either positively or negatively) by binding to miRNA precursors 27 . The TNF gene plays a key role in TMJOA development, as previously reported 28,29 . Interestingly, TNF-α, one of the essential inflammation cytokines present at substantially higher levels in the TMJOA synovium than in the control synovium ( Fig.…”

Section: Mir-330-3p Directly Targets the Tnf Gene And Regulates Tnf-αmentioning

confidence: 60%

CircGCN1L1 promotes synoviocyte proliferation and chondrocyte apoptosis by targeting miR-330-3p and TNF-α in TMJ osteoarthritis

Zhu

Wang

et al. 2020

Cell Death Dis

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Altered expression of circular RNAs (circRNAs) has been identified in various human diseases. In this study, we investigated whether circRNAs function as competing endogenous RNAs to regulate the pathological process of temporomandibular joint osteoarthritis (TMJOA). High-throughput sequencing of mRNA (RNA seq) was performed to detect the expression of circRNAs in TMJOA and control synovial tissues isolated from humans. The differentially upregulated circGCN1L1 (hsa_circ_0000448) in synoviocyte was validated in vitro and in vivo. Here we demonstrate the interactions between circGCN1L1 and both miR-330-3p and tumor necrosis factor-α (TNF-α) through bioinformatics predictions, luciferase report assays, and fluorescence in situ hybridization. mRNA expression profiles of TNF-α-stimulated synoviocyte showed that circGCN1L1 and p65 expressions were upregulated by TNF-α. Moreover, miR-330-3p was negatively correlated with TNF-α secretion. Further, we found that miR-330-3p directly targeted TNF and restrained the production of matrix-degrading enzymes (MMP3, MMP13, and ADAMTS4). Mechanistic studies unveiled that circGCN1L1 in TMJOA synovial tissues and cells may be associated with condylar chondrocyte apoptosis and synoviocyte hyperplasia. Moreover, intra-articular injection of shcircGCN1L1 alleviated TMJOA progression in rat models. Altogether, we elucidated the important roles of a novel circRNA, namely, circGCN1L1, which induced inflammation in TMJ synoviocytes and decreased anabolism of the extracellular matrix (ECM) through miR-330-3p and TNF-α gene. This circRNA may represent a potentially effective therapeutic strategy against TMJOA progression at an early stage.

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“…r-ESW has been demonstrated to clinically accelerate the healing of meniscus tears and muscle injuries and has chondroprotective effects [ 7 , 35 ]. Intriguingly, r-ESW can even strengthen the stemness of stem cells through its unique mechanical characteristics [ 4 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Radial extracorporeal shockwave promotes subchondral bone stem/progenitor cell self-renewal by activating YAP/TAZ and facilitates cartilage repair in vivo

Zhao

Wang

et al. 2021

Stem Cell Res Ther

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Background Radial extracorporeal shockwave (r-ESW), an innovative and noninvasive technique, is gaining increasing attention in regenerative medicine due to its mechanobiological effects. Subchondral bone stem/progenitor cells (SCB-SPCs), originating from the pivotal zone of the osteochondral unit, have been shown to have multipotency and self-renewal properties. However, thus far, little information is available regarding the influences of r-ESW on the biological properties of SCB-SPCs and their therapeutic effects in tissue regeneration. Methods SCB-SPCs were isolated from human knee plateau osteochondral specimens and treated with gradient doses of r-ESW in a suspension stimulation system. The optimized parameters for SCB-SPC self-renewal were screened out by colony-forming unit fibroblast assay (CFU-F). Then, the effects of r-ESW on the proliferation, apoptosis, and multipotency of SCB-SPCs were evaluated. Moreover, the repair efficiency of radial shockwave-preconditioned SCB-SPCs was evaluated in vivo via an osteochondral defect model. Potential mechanisms were explored by western blotting, confocal laser scanning, and high-throughput sequencing. Results The CFU-F data indicate that r-ESW could augment the self-renewal of SCB-SPCs in a dose-dependent manner. The CCK-8 and flow cytometry results showed that the optimized shockwave markedly promoted SCB-SPC proliferation but had no significant influence on cell apoptosis. Radial shockwave exerted no significant influence on osteogenic capacity but strongly suppressed adipogenic ability in the current study. For chondrogenic potentiality, the treated SCB-SPCs were mildly enhanced, while the change was not significant. Importantly, the macroscopic scores and further histological analysis strongly demonstrated that the in vivo therapeutic effects of SCB-SPCs were markedly improved post r-ESW treatment. Further analysis showed that the cartilage-related markers collagen II and proteoglycan were expressed at higher levels compared to their counterpart group. Mechanistic studies suggested that r-ESW treatment strongly increased the expression of YAP and promoted YAP nuclear translocation in SCB-SPCs. More importantly, self-renewal was partially blocked by the YAP-specific inhibitor verteporfin. Moreover, the high-throughput sequencing data indicated that other self-renewal-associated pathways may also be involved in this process. Conclusion We found that r-ESW is capable of promoting the self-renewal of SCB-SPCs in vitro by targeting YAP activity and strengthening its repair efficiency in vivo, indicating promising application prospects.

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Protective effects of extracorporeal shockwave on rat chondrocytes and temporomandibular joint osteoarthritis; preclinical evaluation with in vivo 99mTc-HDP SPECT and ex vivo micro-CT

Cited by 26 publications

References 36 publications

Extracorporeal shockwave treatment in knee osteoarthritis: therapeutic effects and possible mechanism

Extracorporeal shockwave treatment in knee osteoarthritis: therapeutic effects and possible mechanism

CircGCN1L1 promotes synoviocyte proliferation and chondrocyte apoptosis by targeting miR-330-3p and TNF-α in TMJ osteoarthritis

Radial extracorporeal shockwave promotes subchondral bone stem/progenitor cell self-renewal by activating YAP/TAZ and facilitates cartilage repair in vivo

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